Skip to main content
NCBI home page
Annals of Surgery logoLink to Annals of Surgery
. 1979 Jul;190(1):13–17. doi: 10.1097/00000658-197907000-00003

Protease inhibitors and experimental acute hemorrhagic pancreatitis.

T Manabe, M L Steer
PMCID: PMC1344448  PMID: 313758

Abstract

Proteolytic enzyme inhibitors have been reported to decrease morbidity and mortality from certain types of experimental pancreatitis, although recent randomized trials have been unable to demonstrate that they are of benefit in the treatment of clinical acute pancreatitis. We have evaluated the effect of two proteolytic enzyme inhibitors (trasylol and chlorophyll-a, on experimental acute pancreatitis induced in mice by the feeding of a choline-deficient ethionine-enriched diet. The mortality rate and the biochemical and morphological severity of pancreatitis were not altered by either trasylol or chlorophyll-a administration. Thus, in this respect, diet-induced pancreatitis appears to resemble clinical acute pancreatitis. The reasons for the lack of effectiveness of proteolytic enzyme inhibitors in the treatment of both forms of pancreatitis are discussed.

Full text

PDF
13

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEDACHT R. Pankreatitis-Behandlung mit einem Kallikrein-Inaktivator. Arztl Forsch. 1958 Jul 10;12(7):I/371–I/376. [PubMed] [Google Scholar]
  2. Dembińska-Kieć A. The influence of aprotinin (Trasylol) on experimental acute necrosis of the pancreas in rats. Acta Med Pol. 1972;13(1):1–28. [PubMed] [Google Scholar]
  3. Glazer G., Steer M. L. Requirements for activation of trypsinogen and chymotrypsinogen in rabbit pancreatic juice. Anal Biochem. 1977 Jan;77(1):130–140. doi: 10.1016/0003-2697(77)90297-4. [DOI] [PubMed] [Google Scholar]
  4. HUMMEL B. C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959 Dec;37:1393–1399. [PubMed] [Google Scholar]
  5. Imrie C. W., Benjamin I. S., Ferguson J. C., McKay A. J., Mackenzie I., O'Neill J., Blumgart L. H. A single-centre double-blind trial of Trasylol therapy in primary acute pancreatitis. Br J Surg. 1978 May;65(5):337–341. doi: 10.1002/bjs.1800650514. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lombardi B., Estes L. W., Longnecker D. S. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet. Am J Pathol. 1975 Jun;79(3):465–480. [PMC free article] [PubMed] [Google Scholar]
  8. Lombardi B., Rao N. K. Acute hemorrhagic pancreatic necrosis in mice. Influence of the age and sex of the animals and of dietary ethionine, choline, methionine, and adenine sulfate. Am J Pathol. 1975 Oct;81(1):87–100. [PMC free article] [PubMed] [Google Scholar]
  9. MCCUTCHEON A. D. EXPERIMENTAL PANCREATITIS: USE OF A NEW ANTIPROTEOLYTIC SUBSTANCE, TRASYLOL. Ann Surg. 1963 Aug;158:233–239. doi: 10.1097/00000658-196308000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Manabe T., Steer M. L. Experimental acute pancreatitis in mice. Protective effects of glucagon. Gastroenterology. 1979 Mar;76(3):529–534. [PubMed] [Google Scholar]
  11. NEMIR P., Jr, HOFERICHTER J., DRABKIN D. L. THE PROTECTIVE EFFECT OF PROTEINASE INHIBITOR IN ACUTE NECROTIZING PANCREATITIS: AN EXPERIMENTAL STUDY. Ann Surg. 1963 Oct;158:655–665. doi: 10.1097/00000658-196310000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NUGENT F. W., HENDERSON M. E., JONASSON H., JONES C. F., ATENDIDO W. A. THE EFFECT OF A KALLIKREIN-TRYPSIN INACTIVATOR IN EXPERIMENTAL PANCREATITIS. Lahey Clin Found Bull. 1964 Jan-Mar;13:167–171. [PubMed] [Google Scholar]
  13. Rao K. N., Tuma J., Lombardi B. Acute hemorrhagic pancreatic necrosis in mice. Intraparenchymal activation of zymogens, and other enzyme changes in pancreas and serum. Gastroenterology. 1976 May;70(5 PT1):720–726. [PubMed] [Google Scholar]
  14. Trapnell J. E., Rigby C. C., Talbot C. H., Duncan E. H. A controlled trial of Trasylol in the treatment of acute pancreatitis. Br J Surg. 1974 Mar;61(3):177–182. doi: 10.1002/bjs.1800610303. [DOI] [PubMed] [Google Scholar]
  15. WERLE E., TAUBER K., HARTENBACH W., FORELL M. M. Zur Frage der Pankreatitis. Munch Med Wochenschr. 1958 Aug 29;100(35):1265–1267. [PubMed] [Google Scholar]
  16. Wiznitzer T., Orda F. R., Bawnik J. B., Bubis J. J. Acute necrotizing pancreatitis in the guinea pig. Effect of chlorophyll-alpha on survival times. Am J Dig Dis. 1976 Jun;21(6):459–464. doi: 10.1007/BF01072129. [DOI] [PubMed] [Google Scholar]

Articles from Annals of Surgery are provided here courtesy of Lippincott, Williams, and Wilkins

RESOURCES