| Time | Mobile Phase Composition (% B) |
| 0–5.0 min | 100% B |
| 5.0–7.5 min | linear gradient to 0% B |
| 7.5–9.0 min | held at 0% B |
| 9.0–9.001 min | ramp to 100% B |
| 9.001–11.0 min | re-equilibration at 100% B |
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