This is a correction to: Kelly M Harkins, Nathan K Schaefer, Christopher J Troll, Varsha Rao, Joshua Kapp, Colin Naughton, Beth Shapiro, Richard E Green, A novel NGS library preparation method to characterize native termini of fragmented DNA, Nucleic Acids Research, Volume 48, Issue 8, 07 May 2020, Page e47, https://doi.org/10.1093/nar/gkaa128
The colour legends in Figures 3 and 4 were inadvertently inverted during the revision process. Specifically, 3’ overhangs should be indicated in blue and 5’ overhangs in red. The authors have provided corrected versions of both figures, which are presented below. The figure captions remain unchanged and are included for reference.
Revised Figure 3.
Restriction digest experiments. (a) Overhang counts, divided by total per library, across two replicate libraries for 100% mechanically sheared DNA. (b) Overhang counts, divided by total per library, across two replicate libraries for 100% MluCI digested DNA. Values are means across two libraries; error bars show maximum and minimum value. (c) MluCI target sequence abundance with increasing concentration of MluCI. As the percentage of MluCI digested DNA increases (x-axis), so does the frequency of its target sequence (AATT) among 5′ overhang sequences (y-axis). (d) MluCI target sequence is identifiable even in 1% MluCI digested DNA. Points are counts of individual overhang sequences, divided by the sum of all such counts per library. Mean counts across two replicate libraries of only mechanically sheared DNA (x-axis) are shown against mean counts across two replicate libraries of 1% MluCI digested DNA (y-axis). The percent error of each count in 1% MluCI digested DNA was computed, using the count in mechanically sheared DNA as the expected value. All sequences for which this value, rounded to the thousandths place, fell at or above the 99.9th percentile of the distribution are shown labeled. The target sequence (AATT) has the highest percent error (6.2%) and is the only sequence with a value at or above the 99.9th percentile of the distribution (P < 0.001).
Revised Figure 4.
Overhangs created by enzymatic DNA shearing. Overhang counts, base composition of overhangs, and overhang sequences in a gDNA pool treated with the DNaseI (a–c) and Micrococcal nuclease (d–f). Input DNA for all libraries is human genomic DNA extracted from GM12878 cells. All results are the average of two independent libraries; error bars on the overhang abundance plots show maximum and minimum value. In c and f, we computed the difference between the normalized 3′ and 5′ count of each overhang sequence, divided by the mean of the two counts. Shown in text in C and F are sequences for which this value was significant (above the upper 99th percentile of the distribution).
This correction does not affect the results, discussion and conclusions presented in the article. These details have been corrected only in this correction notice to preserve the published version of record.