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. Author manuscript; available in PMC: 2026 Aug 18.
Published in final edited form as: Cell Rep. 2026 Jul 15;45(7):117699. doi: 10.1016/j.celrep.2026.117699

Figure 4. ABL1 kinase associates with and promotes RAD51 and RAD52 tyrosine phosphorylation.

Figure 4.

(A and B) 293T cells were transfected with either Flag-RAD51 (A) or GFP-RAD52 (B) and co-transfected with GFP-ABL1-wild type (ABL-WT), or GFP-active ABL1 (ABL-PP) or GFP-kinase-dead ABL1 (ABL-KM) as indicated. Immunoprecipitation (IP) was performed using anti-Flag (A) or anti-RAD52 (B). All IP-products and whole cell lysates (WCL) were analyzed by western blotting with indicated antibodies.

(C) 293T cells were transfected with either Flag-RAD51-WT or Flag-RAD51-YF (Y315F) mutant and co-transfected with or without ABL-PP (GFP-active-ABL1). Immunoprecipitation (IP) was performed using anti-Flag antibody. All IP-products and whole cell lysates (WCL) were analyzed by western blotting with indicated antibodies.

(D) 293 T cells were transfected with either GFP-RAD52-WT or GFP-RAD52-YF (Y104F) mutant and co-transfected with or without ABL-PP (pCDNA-ABL1-PP). Immunoprecipitation (IP) was performed using anti-GFP antibody. All IP-products and whole cell lysates (WCL) were analyzed by western blotting with indicated antibodies. Vinculin and Actin were used as loading control.

(E) H69 cells were treated with or without ABL001 (20 μM) for 24 h and endogenous RAD51 or RAD52 were immunoprecipitated using anti-RAD51 or anti-RAD52 antibodies. Endogenous IP products and WCL were analyzed by western blotting with indicated antibodies (top panel). Phospho-RAD51 and phospho-RAD52 were quantified and normalized to total RAD51 and RAD52 in IP samples. Expression levels were analyzed using FIJI software, samples without ABL001 treatment were set as 1 (bottom panel).