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. 1975 Aug;250(1):65–84. doi: 10.1113/jphysiol.1975.sp011043

Chloride and water distribution in human red cells.

M Dalmark
PMCID: PMC1348339  PMID: 240930

Abstract

1. The influence of the pH and the temperature on the cellular chloride and water content was investigated in human red cells, the cellular KC1 concentrations of which varied between 10 and 600 mM. The compositions of the media (10-600 mM-KC1, 1 mM-NaC1, 27 mM sucrose) at each single KC1 concentration were constant at the different values of the pH and the temperature. 2. The variation of the cellular KC1 concentration was obtained by washing the cells with KC1 salt solutions containing nystatin...

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTONINI E., WYMAN J., BRUNORI M., FRONTICELLI C., BUCCI E., ROSSI-FANELLI A. STUDIES ON THE RELATIONS BETWEEN MOLECULAR AND FUNCTIONAL PROPERTIES OF HEMOGLOBIN. V. THE INFLUENCE OF TEMPERATURE ON THE BOHR EFFECT IN HUMAN AND IN HORSE HEMOGLOBIN. J Biol Chem. 1965 Mar;240:1096–1103. [PubMed] [Google Scholar]
  2. BRAUNITZER G., GEHRING-MUELLER R., HILSCHMANN N., HILSE K., HOBOM G., RUDLOFF V., WITTMANN-LIEBOLD B. [The structure of normal adult human hemoglobins]. Hoppe Seylers Z Physiol Chem. 1961 Sep 20;325:283–286. doi: 10.1515/bchm2.1961.325.1.283. [DOI] [PubMed] [Google Scholar]
  3. Cass A., Dalmark M. Equilibrium dialysis of ions in nystatin-treated red cells. Nat New Biol. 1973 Jul 11;244(132):47–49. doi: 10.1038/newbio244047a0. [DOI] [PubMed] [Google Scholar]
  4. Cook J. S. Nonsolvent water in human erythrocytes. J Gen Physiol. 1967 May;50(5):1311–1325. doi: 10.1085/jgp.50.5.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DICK D. A., LOWENSTEIN L. M. Osmotic equilibria in human erythrocytes studied by immersion refractometry. Proc R Soc Lond B Biol Sci. 1958 Feb 18;148(931):241–256. doi: 10.1098/rspb.1958.0016. [DOI] [PubMed] [Google Scholar]
  6. Funder J., Wieth J. O. Effect of ouabain on gluclose metabolism and on fluxes of sodium and potassium of human blood cells. Acta Physiol Scand. 1967 Sep;71(1):113–124. doi: 10.1111/j.1748-1716.1967.tb03716.x. [DOI] [PubMed] [Google Scholar]
  7. Funder J., Wieth J. O. Potassium, sodium, and water in normal human red blood cells. Scand J Clin Lab Invest. 1966;18(2):167–180. doi: 10.3109/00365516609051812. [DOI] [PubMed] [Google Scholar]
  8. Gary-Bobo C. M., Solomon A. K. Properties of hemoglobin solutions in red cells. J Gen Physiol. 1968 Nov;52(5):825–853. doi: 10.1085/jgp.52.5.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HARRIS E. J., MAIZELS M. Distribution of ions in suspensions of human erythrocytes. J Physiol. 1952 Sep;118(1):40–53. doi: 10.1113/jphysiol.1952.sp004771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEFEVRE P. G. THE OSMOTICALLY FUNCTIONAL WATER CONTENT OF THE HUMAN ERYTHROCYTE. J Gen Physiol. 1964 Jan;47:585–603. doi: 10.1085/jgp.47.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PONDER E., BARRETO D. The behavior, as regards shape and volume, of human red cell ghosts in fresh and in stored blood. Blood. 1957 Nov;12(11):1016–1027. [PubMed] [Google Scholar]
  12. ROSENTHAL T. B. The effect of temperature on the pH of blood and plasma in vitro. J Biol Chem. 1948 Mar;173(1):25–30. [PubMed] [Google Scholar]
  13. SAVITZ D., SIDEL V. W., SOLOMON A. K. OSMOTIC PROPERTIES OF HUMAN RED CELLS. J Gen Physiol. 1964 Sep;48:79–94. doi: 10.1085/jgp.48.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Siggaard-Andersen O., Salling N. Oxygen-linked hydrogen ion binding of human hemoglobin. Effects of carbon dioxide and 2,3-diphosphoglycerate. II. Studies on whole blood. Scand J Clin Lab Invest. 1971 Jun;27(4):361–366. doi: 10.3109/00365517109080231. [DOI] [PubMed] [Google Scholar]
  15. Tanford C., Nozaki Y. Titration of the histidyl and alpha-amino groups of hemoglobin. J Biol Chem. 1966 Jun 25;241(12):2832–2839. [PubMed] [Google Scholar]
  16. Warburg E. J. Studies on Carbonic Acid Compounds and Hydrogen Ion Activities in Blood and Salt Solutions. A Contribution to the Theory of the Equation of Lawrence J. Henderson and K. A. Hasselbach: CHAP. XII. Biochem J. 1922;16(2):307–340. doi: 10.1042/bj0160307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Winterhalter K. H., Colosimo A. Chromatographic isolation and characterization of isolated chains from hemoglobin after regeneration of sulfhydryl groups. Biochemistry. 1971 Feb 16;10(4):621–624. doi: 10.1021/bi00780a012. [DOI] [PubMed] [Google Scholar]

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