Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1975 Aug;250(1):85–120. doi: 10.1113/jphysiol.1975.sp011044

Actions of gamma-aminobutyric acid on sympathetic ganglion cells.

P R Adams, D A Brown
PMCID: PMC1348340  PMID: 1177140

Abstract

1. Responses of single ganglion cells in the isolated rat superior cervical ganglion to gamma-aminobutyric acid (GABA) applied via the bathing medium were recorded using intracellular micro-electrodes. 2. GABA produced a large fall in cell input resistance, frequently to immeasurable levels. In thirteen cells showing a modest response to 100 muM GABA, input resistance fell from 50-5 +/-9-5 to 15.9 +/- 3-2 Momega (means +/- S.E. of mean). After correction for resistance leaks introduced by the impaling electrode, the resting membrane resistance Rm and the resistance of the GABA-shunt Rg in these cells were calculated to be 79-3 +/- 16-6 and 35-0 +/- 9-5 Momega respectively. 3. Cells with recorded resting membrane potentials greater than -42 mV were depolarized by GABA; at resting potential less than -42 mV they were hyperpolarized...

Full text

PDF
85

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams P. R., Brown D. A. Action of -aminobutyric acid (GABA) on rat sympathetic ganglion cells. Br J Pharmacol. 1973 Mar;47(3):639P–640P. [PMC free article] [PubMed] [Google Scholar]
  2. Adams P. R., Brown D. A. Proceedings: Some observations on electrically-inexcitable cells (neuroglia?) in rat sympathetic ganglia. Br J Pharmacol. 1974 May;51(1):131P–132P. [PMC free article] [PubMed] [Google Scholar]
  3. Adrian R. H., Costantin L. L., Peachey L. D. Radial spread of contraction in frog muscle fibres. J Physiol. 1969 Sep;204(1):231–257. doi: 10.1113/jphysiol.1969.sp008910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLACKMAN J. G., GINSBORG B. L., RAY C. Some effects of changes in ionic concentration on the action potential of sympathetic ganglion cells in the frog. J Physiol. 1963 Jul;167:374–388. doi: 10.1113/jphysiol.1963.sp007156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BLACKMAN J. G., GINSBORG B. L., RAY C. Synaptic transmission in the sympathetic ganglion of the frog. J Physiol. 1963 Jul;167:355–373. doi: 10.1113/jphysiol.1963.sp007155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Barker J. L., Nicoll R. A. The pharmacology and ionic dependency of amino acid responses in the frog spinal cord. J Physiol. 1973 Jan;228(2):259–277. doi: 10.1113/jphysiol.1973.sp010085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Baylor D. A., Fuortes M. G., O'Bryan P. M. Receptive fields of cones in the retina of the turtle. J Physiol. 1971 Apr;214(2):265–294. doi: 10.1113/jphysiol.1971.sp009432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Blackman J. G., Purves R. D. Intracellular recordings from ganglia of the thoracic sympathetic chain of the guinea-pig. J Physiol. 1969 Jul;203(1):173–198. doi: 10.1113/jphysiol.1969.sp008858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bowery N. G., Brown D. A. -Aminobutyric acid uptake by sympathetic ganglia. Nat New Biol. 1972 Jul 19;238(81):89–91. doi: 10.1038/newbio238089a0. [DOI] [PubMed] [Google Scholar]
  10. Bowery N. G., Brown D. A. Depolarizing actions of gamma-aminobutyric acid and related compounds on rat superior cervical ganglia in vitro. Br J Pharmacol. 1974 Feb;50(2):205–218. doi: 10.1111/j.1476-5381.1974.tb08563.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Brown D. A., Brownstein M. J., Scholfield C. N. Origin of the after-hyperpolarization that follows removal of depolarizing agents from the isolated superior cervical ganglion of the rat. Br J Pharmacol. 1972 Apr;44(4):651–671. doi: 10.1111/j.1476-5381.1972.tb07305.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Brown D. A., Scholfield C. N. Changes of intracellular sodium and potassium ion concentrations in isolated rat superior cervical ganglia induced by depolarizing agents. J Physiol. 1974 Oct;242(2):307–319. doi: 10.1113/jphysiol.1974.sp010709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Brown D. A., Scholfield C. N. Movements of labelled sodium ions in isolated rat superior cervical ganglia. J Physiol. 1974 Oct;242(2):321–351. doi: 10.1113/jphysiol.1974.sp010710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cole K. S., Curtis H. J. ELECTRIC IMPEDANCE OF THE SQUID GIANT AXON DURING ACTIVITY. J Gen Physiol. 1939 May 20;22(5):649–670. doi: 10.1085/jgp.22.5.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Dreifuss J. J., Kelly J. S., Krnjević K. Cortical inhibition and gamma-aminobutyric acid. Exp Brain Res. 1969;9(2):137–154. doi: 10.1007/BF00238327. [DOI] [PubMed] [Google Scholar]
  16. Engel E., Barcilon V., Eisenberg R. S. The interpretation of current-voltage relations recorded from a spherical cell with a single microelectrode. Biophys J. 1972 Apr;12(4):384–403. doi: 10.1016/S0006-3495(72)86091-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Epstein R., Grundfest H. Desensitization of gamma aminobutyric acid (GABA) receptors in muscle fibers of the crab Cancer borealis. J Gen Physiol. 1970 Jul;56(1):33–45. doi: 10.1085/jgp.56.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ginsborg B. L. Electrical changes in the membrane in junctional transmission. Biochim Biophys Acta. 1973 Nov 28;300(3):289–317. doi: 10.1016/0304-4157(73)90007-5. [DOI] [PubMed] [Google Scholar]
  19. Ginsborg B. L., House C. R., Silinsky E. M. Conductance changes associated with the secretory potential in the cockroach salivary gland. J Physiol. 1974 Feb;236(3):723–731. doi: 10.1113/jphysiol.1974.sp010462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ginsborg B. L. Ion movements in junctional transmission. Pharmacol Rev. 1967 Sep;19(3):289–316. [PubMed] [Google Scholar]
  21. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. KEYNES R. D. CHLORIDE IN THE SQUID GIANT AXON. J Physiol. 1963 Dec;169:690–705. doi: 10.1113/jphysiol.1963.sp007289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Koketsu K. Cholinergic synaptic potentials and the underlying ionic mechasims. Fed Proc. 1969 Jan-Feb;28(1):101–112. [PubMed] [Google Scholar]
  25. Krnjević K., Schwartz S. The action of gamma-aminobutyric acid on cortical neurones. Exp Brain Res. 1967;3(4):320–336. doi: 10.1007/BF00237558. [DOI] [PubMed] [Google Scholar]
  26. MARTIN A. R., PILAR G. DUAL MODE OF SYNAPTIC TRANSMISSION IN THE AVIAN CILIARY GANGLION. J Physiol. 1963 Sep;168:443–463. doi: 10.1113/jphysiol.1963.sp007202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. McLachlan E. M. The formation of synapses in mammalian sympathetic ganglia reinnervated with preganglionic or somatic nerves. J Physiol. 1974 Feb;237(1):217–242. doi: 10.1113/jphysiol.1974.sp010479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nishi S., Minota S., Karczmar A. G. Primary afferent neurones: the ionic mechanism of GABA-mediated depolarization. Neuropharmacology. 1974 Mar;13(3):215–219. doi: 10.1016/0028-3908(74)90110-5. [DOI] [PubMed] [Google Scholar]
  29. Obata K. Transmitter sensitivities of some nerve and muscle cells in culture. Brain Res. 1974 Jun 14;73(1):71–88. doi: 10.1016/0006-8993(74)91008-7. [DOI] [PubMed] [Google Scholar]
  30. Ozawa S., Tsuda K. Membrane permeability change during inhibitory transmitter action in crayfish stretch receptor cell. J Neurophysiol. 1973 Sep;36(5):805–816. doi: 10.1152/jn.1973.36.5.805. [DOI] [PubMed] [Google Scholar]
  31. Perri V., Sacchi O., Caella C. Electrical properties and synaptic connections of the sympathetic neurons in the rat and guinea-pig superior cervical ganglion. Pflugers Arch. 1970;314(1):40–54. doi: 10.1007/BF00587045. [DOI] [PubMed] [Google Scholar]
  32. Russell J. M., Brown A. M. Active transport of chloride by the giant neuron of the Aplysia abdominal ganglion. J Gen Physiol. 1972 Nov;60(5):499–518. doi: 10.1085/jgp.60.5.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sacchi O., Perri V. Quantal release of acetylcholine from the nerve endings of the guinea-pig superior cervical ganglion. Pflugers Arch. 1971;329(3):207–219. doi: 10.1007/BF00586615. [DOI] [PubMed] [Google Scholar]
  34. Schanne O., Kawata H., Schäfer B., Lavallée M. A study on the electrical resistance of the frog sartorius muscle. J Gen Physiol. 1966 May;49(5):897–912. doi: 10.1085/jgp.49.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Stoney S. D., Jr, Machne X. Mechanisms of accommodation in different types of frog neurons. J Gen Physiol. 1969 Feb;53(2):248–262. doi: 10.1085/jgp.53.2.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Woodward J. K., Bianchi C. P., Erulkar S. D. Electrolyte distribution in rabbit superior cervical ganglion. J Neurochem. 1969 Mar;16(3):289–299. doi: 10.1111/j.1471-4159.1969.tb10367.x. [DOI] [PubMed] [Google Scholar]
  37. Young J. A., Brown D. A., Kelly J. S., Schon F. Autoradiographic localization of sites of (3H)gamma-aminobutyric acid accumulation in peripheral autonomic ganglia. Brain Res. 1973 Dec 7;63:479–486. doi: 10.1016/0006-8993(73)90128-5. [DOI] [PubMed] [Google Scholar]
  38. de Groat W. C. The actions of gamma-aminobutyric acid and related amino acids on mammalian autonomic ganglia. J Pharmacol Exp Ther. 1970 Apr;172(2):384–396. [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES