Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1975 Sep;250(2):373–383. doi: 10.1113/jphysiol.1975.sp011060

Regional differences in the effect of bile salts on absorption by rat small intestine in vivo.

D R Saunders
PMCID: PMC1348367  PMID: 1177146

Abstract

1. The hypothesis that endogenous bile salts influence water absorption in rat small intestine was tested in vivo. 2. Net water transport was measured under steady-state conditions during single-pass infusion of segments from three regions of the small intestine. Each segment served as its own control. 3. Delipidated rat bile, and solutions of the principal bile salts of the rat, taurocholate and glycocholate, reduced absorption in the proximal jejunum but not in the distal ileum. 4. 1-Palmitoyl,2-oleoyl lecithin, an important biliary lecithin, counteracted the inhibitory effect of taurocholate and of glycocholate on absorption. The presence of biliary lecithins probably explains why whole rat bile did not depress water absorption in the proximal jejunum. 5. Net water transport in the distal jejunum was inversely related to log10 (taurocholate concentration). 6. Endogenous bile salts may help to maintain the fluidity of the contents of the mid-small intestine in the rat after biliary and dietary lecithins have been absorbed.

Full text

PDF
373

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnesjö B., Nilsson A., Barrowman J., Borgström B. Intestinal digestion and absorption of cholesterol and lecithin in the human. Intubation studies with a fat-soluble reference substance. Scand J Gastroenterol. 1969;4(8):653–665. doi: 10.3109/00365526909180651. [DOI] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. BORGSTROM B., DAHLQVIST A., LUNDH G., SJOVALL J. Studies of intestinal digestion and absorption in the human. J Clin Invest. 1957 Oct;36(10):1521–1536. doi: 10.1172/JCI103549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Binder H. J., Rawlins C. L. Effect of conjugated dihydroxy bile salts on electrolyte transport in rat colon. J Clin Invest. 1973 Jun;52(6):1460–1466. doi: 10.1172/JCI107320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Booth C. C., Read A. E., Jones E. Studies on the site of fat absorption: 1. The sites of absorption of increasing doses of I-labelled triolein in the rat. Gut. 1961 Mar;2(1):23–31. doi: 10.1136/gut.2.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bright-Asare P., Binder H. J. Stimulation of colonic secretion of water and electrolytes by hydroxy fatty acids. Gastroenterology. 1973 Jan;64(1):81–88. [PubMed] [Google Scholar]
  7. Carter G. W., Van Dyke K. A superior counting solution for water-soluble tritiated compounds. Clin Chem. 1971 Jul;17(7):576–580. [PubMed] [Google Scholar]
  8. Dietschy J. M. Effects of bile salts on intermediate metabolism of the intestinal mucosa. Fed Proc. 1967 Nov-Dec;26(6):1589–1598. [PubMed] [Google Scholar]
  9. Forth W., Rummel W., Glasner H. Zur resorptionshemmenden Wirkung von Gallensäuren. Naunyn Schmiedebergs Arch Pharmakol Exp Pathol. 1966;254(4):364–380. [PubMed] [Google Scholar]
  10. Goodwin J. F. Method for simultaneous direct estimation of glucose and xylose in serum. Clin Chem. 1970 Feb;16(2):85–91. [PubMed] [Google Scholar]
  11. Harries J. T., Sladen G. E. The effects of different bile salts on the absorption of fluid, electrolytes, and monosaccharides in the small intestine of the rat in vivo. Gut. 1972 Aug;13(8):596–603. doi: 10.1136/gut.13.8.596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kiekens R., Wissocq P., Govaerts J. P. Lipase and bile salts in the small intestine of the dog. Relation to lipid absorption. Digestion. 1971;4(5):295–308. doi: 10.1159/000197132. [DOI] [PubMed] [Google Scholar]
  13. Krag E., Phillips S. F. Effect of free and conjugated bile acids on net water, electrolyte, and glucose movement in the perfused human ileum. J Lab Clin Med. 1974 Jun;83(6):947–956. [PubMed] [Google Scholar]
  14. LACK L., WEINER I. M. In vitro absorption of bile salts by small intestine of rats and guinea pigs. Am J Physiol. 1961 Feb;200:313–317. doi: 10.1152/ajplegacy.1961.200.2.313. [DOI] [PubMed] [Google Scholar]
  15. LIN T. H., RUBINSTEIN R., HOLMES W. L. A STUDY OF THE EFFECT OF D- AND L-TRIIODOTHYRONINE ON BILE ACID EXCRETION OF RATS. J Lipid Res. 1963 Jan;4:63–67. [PubMed] [Google Scholar]
  16. Mekhjian H. S., Phillips S. F. Perfusion of the canine colon with unconjugated bile acids. Effect on water and electrolyte transport, morphology, and bile acid absorption. Gastroenterology. 1970 Jul;59(1):120–129. [PubMed] [Google Scholar]
  17. Mekjian H. S., Phillips S. F., Hofmann A. F. Colonic secretion of water and electrolytes induced by bile acids: perfusion studies in man. J Clin Invest. 1971 Aug;50(8):1569–1577. doi: 10.1172/JCI106644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miettinen T. A., Peltokallio P. Bile salt, fat, water, and vitamin B 12 excretion after ileostomy. Scand J Gastroenterol. 1971;6(6):543–552. doi: 10.3109/00365527109181671. [DOI] [PubMed] [Google Scholar]
  19. NISSIM J. A. Reduction of the intestinal absorption of glucose, methionine and sodium butyrate by the cation trimethyl-hexadecylammonium. Nature. 1960 Jul 23;187:308–310. doi: 10.1038/187308a0. [DOI] [PubMed] [Google Scholar]
  20. Russell R. I., Allan J. G., Gerskowitch V. P., Cochran K. M. The effect of conjugated and unconjugated bile acids on water and electrolyte absorption in the human jejunum. Clin Sci Mol Med. 1973 Sep;45(3):301–311. doi: 10.1042/cs0450301. [DOI] [PubMed] [Google Scholar]
  21. Sladen G. E., Harries J. T. Studies on the effects of unconjugated dihydroxy bile salts on rat small intestinal function in vivo. Biochim Biophys Acta. 1972 Nov 2;288(2):443–456. doi: 10.1016/0005-2736(72)90265-9. [DOI] [PubMed] [Google Scholar]
  22. TALALAY P. Enzymic analysis of steroid hormones. Methods Biochem Anal. 1960;8:119–143. doi: 10.1002/9780470110249.ch3. [DOI] [PubMed] [Google Scholar]
  23. Teem M. V., Phillips S. F. Perfusion of the hamster jejunum with conjugated and unconjugated bile acids: inhibition of water absorption and effects on morphology. Gastroenterology. 1972 Feb;62(2):261–267. [PubMed] [Google Scholar]
  24. Wingate D. L., Phillips S. F., Hofmann A. F. Effect of glycine-conjugated bile acids with and without lecithin on water and glucose absorption in perfused human jejunum. J Clin Invest. 1973 May;52(5):1230–1236. doi: 10.1172/JCI107290. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES