S. Hauf, R. Nakamura, B. Cellini, Y. Yokobayashi, and M. Dindo, “Emergent Chemical Reactivity and Complexity of RNA Condensates,” Angewandte Chemie International Edition 64 (2025): e11332, https://doi.org/10.1002/anie.202511332.
In panel d of Figure 2 legend, the text “Representative confocal microscopy images showing the single‐phase (pH > 5, top) and two‐phase (pH < 4, bottom with visible droplets) regimes. Green fluorescence represents FAM‐signal of the labeled hammerhead ribozyme substrate partitioning into the RNA phase. Scale bars are 20 µm.” was incorrect. This should have read: “Representative confocal microscopy images showing the single phase (pH > 5, top) and two‐phase regime (pH < 4, bottom, with visible droplets). Green fluorescence represents the FAM‐signal of the labeled ssDNA (Figure 3i). Scale bars are 20 µm.”
In the Supporting Information, Figure S3 has been replaced in its entirety due to errors in some panels. Additionally, Figures S7 and S11 have been simplified by removing redundant data from panel A of each figure, as the original panels contained overlapping information.
FIGURE S3 Representative microscopic images of RNA condensates obtained at different concentrations of Tris‐HCl buffer pH 8.0, and a graph reporting the pH of the entire solution measured with a pH meter. RNA was used at 2.5 g/L, and the phase separation was triggered using 50 mM MgCl2. Scale bars correspond to 20 µm. Brightness and contrast were adjusted to +80 and +100, respectively, using the GNU Image Manipulation Program (GIMP 2.10.30). The reported pH values are the average and standard deviation from three experiments.
FIGURE S7 Diffusion of small molecules within the RNA condensates. The RNA condensates have been prepared using 5 g/L RNA and 50 mM MgCl2. (a) Diffusion of SNARF‐1 (red) at 79 (top) and 569 s (bottom) within pre‐formed condensates containing FAM‐labeled DNA. Analysis of the intensity of the signal reported as a gray value at two different time points. The red signal refers to SNARF‐1, while the green one to FAM‐labeled DNA partitioned in the condensates. SNARF‐1 was added at a final concentration of 10 µM. (b) Analysis of the diffusion of Rhodamine 6G within two condensates of different sizes at several frames. Rhodamine 6G was used at a final concentration of 20 µM. Scale bars correspond to 20 µm.
FIGURE S11 Localization of fatty acids and phospholipids added to pre‐formed RNA condensates. (a) Localization of undecylic acid (or C11 undecanoic acid) and (b) localization of lauric acid (or C12 dodecanoic acid) on the edges of the RNA condensates after 10–20 min (left panel) and 80 min (right panel). Both fatty acids have been used at a final concentration of 10 µM. (c) Localization of the fatty acids (C11 and C12) on the edges of small condensates. (d) Partitioning of phospholipid (β‐BODIPY C12‐HPC, Hexadecanoyl‐sn‐Glycero‐3‐Phosphocholine, used at a final concentration of 10 µM). Fatty acids and the phospholipids have been added after condensate formation, using both MgCl2 and HCl (both at 50 or 100 mM). Scale bars correspond to 25 µm.
We apologize for these errors.