Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1975 Oct;251(2):287–301. doi: 10.1113/jphysiol.1975.sp011093

Metabolic changes associated with the slowing of relaxation in fatigued mouse muscle.

R H Edwards, D K Hill, D A Jones
PMCID: PMC1348428  PMID: 1185665

Abstract

1. The biochemical basis of the slowing of relaxation seen in fatigue has been examined using an isolated mouse soleus preparation. 2. Slowing of relaxation occurred during prolonged tetani under anaerobic conditions when ATP and PC fell and lactate accumulated. 3. Slowing of relaxation was also demonstrated with muscles poisoned with cyanide and iodoacetic acid when there was a fall in ATP and PC but no accumulation of lactate. During a period of anaerobic recovery following a fatiguing tetanus, relaxation became faster at a time when lactate was accumulating in the muscle. 4. It is concluded that the slowing of relaxation in fatigue is not a consequence of lactate accumulation, and a relationship is demonstrated between the ATP content of the muscle and the rate of relaxation in muscles fatigued by prolonged stimulation, 5. Rates of ATP turn-over in fresh muscle, and at intervals throughout a tetanus are consistent with the suggestion that the rate limiting step for myofibrillar ATPase may be directly related to the rate limiting step for the decay of tension during relaxation.

Full text

PDF
287

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K., KREBS H. A. The free-energy changes associated with the individual steps of the tricarboxylic acid cycle, glycolysis and alcoholic fermentation and with the hydrolysis of the pyrophosphate groups of adenosinetriphosphate. Biochem J. 1953 Apr;54(1):94–107. doi: 10.1042/bj0540094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Canfield P., Maréchal G. Equilibrium of nucleotides in frog sartorius muscle during an isometric tetanus at 20 degrees C. J Physiol. 1973 Aug;232(3):453–466. doi: 10.1113/jphysiol.1973.sp010280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cleworth D. R., Edman K. A. Changes in sarcomere length during isometric tension development in frog skeletal muscle. J Physiol. 1972 Dec;227(1):1–17. doi: 10.1113/jphysiol.1972.sp010016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ebashi S., Endo M., Otsuki I. Control of muscle contraction. Q Rev Biophys. 1969 Nov;2(4):351–384. doi: 10.1017/s0033583500001190. [DOI] [PubMed] [Google Scholar]
  5. Edwards R. H., Jones D. A., Maunder C., Batra G. J. Needle biopsy for muscle chemistry. Lancet. 1975 Mar 29;1(7909):736–740. doi: 10.1016/s0140-6736(75)91642-6. [DOI] [PubMed] [Google Scholar]
  6. Gilbert C., Kretzschmar K. M., Wilkie D. R., Woledge R. C. Chemical change and energy output during muscular contraction. J Physiol. 1971 Oct;218(1):163–193. doi: 10.1113/jphysiol.1971.sp009609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUXLEY A. F. Muscle structure and theories of contraction. Prog Biophys Biophys Chem. 1957;7:255–318. [PubMed] [Google Scholar]
  8. Hill D. K. Resting tension and the form of the twitch of rat skeletal muscle at low temperature. J Physiol. 1972 Feb;221(1):161–171. doi: 10.1113/jphysiol.1972.sp009746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Homsher E., Mommaerts W. F., Ricchiuti N. V., Wallner A. Activation heat, activation metabolism and tension-related heat in frog semitendinosus muscles. J Physiol. 1972 Feb;220(3):601–625. doi: 10.1113/jphysiol.1972.sp009725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huxley A. F., Simmons R. M. Rapid 'give' and the tension 'shoulder' in the relaxation of frog muscle fibres. J Physiol. 1970 Sep;210(1):32P–33P. [PubMed] [Google Scholar]
  11. JEWELL B. R., WILKIE D. R. The mechanical properties of relaxing muscle. J Physiol. 1960 Jun;152:30–47. doi: 10.1113/jphysiol.1960.sp006467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones D. A. Combined techniques for studying the physiology and biochemistry of fatigue in the isolated soleus of the mouse. J Physiol. 1973 Jun;231(2):68P–69P. [PubMed] [Google Scholar]
  13. Julian F. J. Activation in a skeletal muscle contraction model with a modification for insect fibrillar muscle. Biophys J. 1969 Apr;9(4):547–570. doi: 10.1016/S0006-3495(69)86403-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kendrick-Jones J. Role of myosin light chains in calcium regulation. Nature. 1974 Jun 14;249(458):631–634. doi: 10.1038/249631a0. [DOI] [PubMed] [Google Scholar]
  15. Kretzschmar K. M., Wilkie D. R. A new approach to freezing tissues rapidly. J Physiol. 1969 Jun;202(2):66P–67P. [PubMed] [Google Scholar]
  16. Lymn R. W., Taylor E. W. Mechanism of adenosine triphosphate hydrolysis by actomyosin. Biochemistry. 1971 Dec 7;10(25):4617–4624. doi: 10.1021/bi00801a004. [DOI] [PubMed] [Google Scholar]
  17. Perrie W. T., Smillie L. B., Perry S. B. A phosphorylated light-chain component of myosin from skeletal muscle. Biochem J. 1973 Sep;135(1):151–164. doi: 10.1042/bj1350151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pires E., Perry S. V., Thomas M. A. Myosin light-chain kinase, a new enzyme from striated muscle. FEBS Lett. 1974 May 1;41(2):292–296. doi: 10.1016/0014-5793(74)81232-9. [DOI] [PubMed] [Google Scholar]
  19. Rüdel R., Taylor S. R. Aequorin luminescence during contraction of amphibian skeletal muscle. J Physiol. 1973 Aug;233(1):5P–6P. [PubMed] [Google Scholar]
  20. Sandow A. Excitation-contraction coupling in skeletal muscle. Pharmacol Rev. 1965 Sep;17(3):265–320. [PubMed] [Google Scholar]
  21. Smith I. C. Energetics of activation in frog and toad muscle. J Physiol. 1972 Feb;220(3):583–599. doi: 10.1113/jphysiol.1972.sp009724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spande J. I., Schottelius B. A. Chemical basis of fatigue in isolated mouse soleus muscle. Am J Physiol. 1970 Nov;219(5):1490–1495. doi: 10.1152/ajplegacy.1970.219.5.1490. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES