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. 1975 Nov;252(3):657–667. doi: 10.1113/jphysiol.1975.sp011163

Neurotrophic regulation of dynamic properties of skeletal muscle: effects of botulinum toxin and denervation.

D B Drachman, D M Johnston
PMCID: PMC1348488  PMID: 173836

Abstract

In order to determine the role of acetylcholine (ACh) transmission in neurotrophic regulation of dynamic properties of muscle, the effects of botulinum toxin treatment were compared with those of denervation. The extensor digitorum longus (EDL) and soleus muscles of rats were either denervated or injected with botulinum toxin. At times up to 25 days the isometric properties of these muscles were determined. 2. Both botulinum treatment and denervation produced progressive slowing of the time to peak of the twitch (TPT) and half-relaxation time of the twitch (1/2 RT), which was more pronounced in the EDL than in the soleus. 3. Both treatments produced slowing of the relaxation curve following tetanic contraction, more marked in the EDL than in the soleus muscle. This indicates a slowing of relaxation, and suggests a prolongation of the active state of the muscle. 4. The maximum rate of rise of the tetanus did not change significantly in the EDL and soleus muscles after botulinum treatment or denervation. This suggests that there is no major change in the speed of contraction under conditions of botulinum treatment or denervation. 5. The changes produced by botulinum treatment and denervation were virtually identical in all parameters tested. This is interpreted meaning that cholinergic transmission (including muscle usage), or some other factor closely related to cholinergic transmission, accounts for the motor nerve's trophic influence in maintaining these dynamic properties of skeletal muscles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albuquerque E. X., Warnick J. E., Tasse J. R., Sansone F. M. Effects of vinblastine and colchicine on neural regulation of the fast and slow skeletal muscles of the rat. Exp Neurol. 1972 Dec;37(3):607–634. doi: 10.1016/0014-4886(72)90103-3. [DOI] [PubMed] [Google Scholar]
  2. BULLER A. J., ECCLES J. C., ECCLES R. M. Differentiation of fast and slow muscles in the cat hind limb. J Physiol. 1960 Feb;150:399–416. doi: 10.1113/jphysiol.1960.sp006394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BULLER A. J., ECCLES J. C., ECCLES R. M. Interactions between motoneurones and muscles in respect of the characteristic speeds of their responses. J Physiol. 1960 Feb;150:417–439. doi: 10.1113/jphysiol.1960.sp006395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BULLER A. J., LEWIS D. M. THE RATE OF TENSION DEVELOPMENT IN ISOMETRIC TETANIC CONTRACTIONS OF MAMMALIAN FAST AND SLOW SKELETAL MUSCLE. J Physiol. 1965 Feb;176:337–354. doi: 10.1113/jphysiol.1965.sp007554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLOSE R. DYNAMIC PROPERTIES OF FAST AND SLOW SKELETAL MUSCLES OF THE RAT DURING DEVELOPMENT. J Physiol. 1964 Sep;173:74–95. doi: 10.1113/jphysiol.1964.sp007444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Close R. Dynamic properties of fast and slow skeletal muscles of the rat after nerve cross-union. J Physiol. 1969 Oct;204(2):331–346. doi: 10.1113/jphysiol.1969.sp008916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Close R., Hoh J. F. Force: velocity pproperties of kitten muscles. J Physiol. 1967 Oct;192(3):815–822. doi: 10.1113/jphysiol.1967.sp008333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Close R. The relation between intrinsic speed of shortening and duration of the active state of muscle. J Physiol. 1965 Oct;180(3):542–559. doi: 10.1113/jphysiol.1965.sp007716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Drachman D. B., Houk J. Effect of botulinum toxin on speed of skeletal muscle contraction. Am J Physiol. 1969 Jun;216(6):1453–1455. doi: 10.1152/ajplegacy.1969.216.6.1453. [DOI] [PubMed] [Google Scholar]
  11. Drachman D. B., Johnston D. M. Development of a mammalian fast muscle: dynamic and biochemical properties correlated. J Physiol. 1973 Oct;234(1):29–42. doi: 10.1113/jphysiol.1973.sp010332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Drachman D. B. Neurotrophic regulation of muscle cholinesterase: effects of botulinum toxin and denervation. J Physiol. 1972 Nov;226(3):619–627. doi: 10.1113/jphysiol.1972.sp010000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Drachman D. B. Trophic functions of the neuron. 3. Mechanisms of neurotrophic interactions. The role of acetylcholine as a neurotropic transmitter. Ann N Y Acad Sci. 1974 Mar 22;228(0):160–176. doi: 10.1111/j.1749-6632.1974.tb20508.x. [DOI] [PubMed] [Google Scholar]
  14. Fernandez H. L., Ramirez B. U. Muscle fibrillation induced by blockage of axoplasmic transport in motor nerves. Brain Res. 1974 Oct 25;79(3):385–395. doi: 10.1016/0006-8993(74)90436-3. [DOI] [PubMed] [Google Scholar]
  15. Fischbach G. D. The different effect of neuromuscular inactivity and muscle atrophy on speed of contraction. Exp Neurol. 1970 Jul;28(1):189–190. doi: 10.1016/0014-4886(70)90173-1. [DOI] [PubMed] [Google Scholar]
  16. Harris A. J., Miledi R. The effect of type D botulinum toxin on frog neuromuscular junctions. J Physiol. 1971 Sep;217(2):497–515. doi: 10.1113/jphysiol.1971.sp009582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hofmann W. W., Peacock J. H. Postjunctional changes induced by partial interruption of axoplasmic flow in motor nerves. Exp Neurol. 1973 Nov;41(2):345–356. doi: 10.1016/0014-4886(73)90275-6. [DOI] [PubMed] [Google Scholar]
  18. Hofmann W. W., Thesleff S. Studies on the trophic influence of nerve on skeletal muscle. Eur J Pharmacol. 1972 Dec;20(3):256–260. doi: 10.1016/0014-2999(72)90182-3. [DOI] [PubMed] [Google Scholar]
  19. Kean C. J., Lewis D. M., McGarrick J. D. Dynamic properties of denervated fast and slow twitch muscle of the cat. J Physiol. 1974 Feb;237(1):103–113. doi: 10.1113/jphysiol.1974.sp010472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lewis D. M., Kean C. J., McGarrick J. D. Trophic functions of the neuron. II. Denervation and regulation of muscle. Dynamic properties of slow and fast muscle and their trophic regulation. Ann N Y Acad Sci. 1974 Mar 22;228(0):105–120. doi: 10.1111/j.1749-6632.1974.tb20505.x. [DOI] [PubMed] [Google Scholar]
  21. Lewis D. M. The effect of denervation on the mechanical and electrical responses of fast and slow mammalian twitch muscle. J Physiol. 1972 Apr;222(1):51–75. doi: 10.1113/jphysiol.1972.sp009787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lomo T., Westgaard R. H., Dahl H. A. Contractile properties of muscle: control by pattern of muscle activity in the rat. Proc R Soc Lond B Biol Sci. 1974 Aug 27;187(1086):99–103. doi: 10.1098/rspb.1974.0064. [DOI] [PubMed] [Google Scholar]
  23. Spitzer N. Miniature end-plate potentials at mammalian neuromuscular junctions poisoned by botulinum toxin. Nat New Biol. 1972 May 3;237(70):26–27. doi: 10.1038/newbio237026a0. [DOI] [PubMed] [Google Scholar]

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