Table 2.
PCR conditions for 18S rDNA gene region amplification using different primer pairs.
| Primer pair | Initial denaturation | Cycles (denaturation, annealing, elongation) | Final elongation | Reference |
|---|---|---|---|---|
| MyxospecF-18R | 94°C, 3 min | 35 cycles: 95°C 30 s, 55°C 45 s, 72°C 90 sa | 72°C, 7 min | Liu et al. (2016) |
| Myxgen4F-ERIB10 | 94°C, 3 min | 35 cycles: 94°C 45 s, 55°C 50 s, 72°C 1 min | 72°C, 10 min | Colunga-Ramírez et al., 2024 |
| Myx1F-SphR | ||||
| ERIB1-ACT1R | 95°C, 3 min | 35 cycles: 95°C 1 min, 55°C 45 s, 72°C 2 min | 72°C, 7 min | Úngari et al. (2021) |
a
The original protocol was modified with 90 s of elongation period.