Abstract
1. The rate of osmotic influx of water into flexible and hardened erythrocytes has been studied using a stopped-flow rapid-reaction method, and diluting the cells 1:20 in hypotonic media.
2. Neither the initial rate of swelling nor the time to the onset of haemolysis were changed after making the erythrocytes inflexible by adding formaldehyde or by heating at 48° C for 30 min.
3. From a plot of the initial rate of uptake with hypotonicity, the permeability coefficient k can be calculated as 0·098 μ3/μ2. sec atm (± 10%) at 20·5° C.
4. The value of k calculated from the time to the onset of haemolysis is 0·28 μ3/μ2 sec atm. This suggests individual cells may differ over a wide range of permeability.
5. The results are consistent with the pores being at fixed sites in the membrane and unaffected by flexing of the cell.
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Selected References
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- Bowdler A. J., Chan T. K. The time course of red cell lysis in hypotonic electrolyte solutions. J Physiol. 1969 Apr;201(2):437–452. doi: 10.1113/jphysiol.1969.sp008765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fricke H. THE RATE OF ESCAPE OF HEMOGLOBIN FROM THE HEMOLYZED RED CORPUSCLE. J Gen Physiol. 1934 Sep 20;18(1):103–107. doi: 10.1085/jgp.18.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEFEVRE P. G. THE OSMOTICALLY FUNCTIONAL WATER CONTENT OF THE HUMAN ERYTHROCYTE. J Gen Physiol. 1964 Jan;47:585–603. doi: 10.1085/jgp.47.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIDEL V. W., SOLOMON A. K. Entrance of water into human red cells under an osmotic pressure gradient. J Gen Physiol. 1957 Nov 20;41(2):243–257. doi: 10.1085/jgp.41.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIRS J. A., ROUGHTON F. J. Stopped-flow measurements of CO and O2 uptake by hemoglobin in sheep erythrocytes. J Appl Physiol. 1963 Jan;18:158–165. doi: 10.1152/jappl.1963.18.1.158. [DOI] [PubMed] [Google Scholar]
- Sha'afi R. I., Rich G. T., Sidel V. W., Bossert W., Solomon A. K. The effect of the unstirred layer on human red cell water permeability. J Gen Physiol. 1967 May;50(5):1377–1399. doi: 10.1085/jgp.50.5.1377. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sirs J. A. The egress of oxygen from sheep erythrocytes. Bibl Laeger. 1966 Mar 14;112(3):538–549. doi: 10.1016/0926-6585(66)90257-3. [DOI] [PubMed] [Google Scholar]
- Sirs J. A. The measurement of the haematocrit and flexibility of erythrocytes with a centrifuge. Biorheology. 1968 Mar;5(1):1–14. doi: 10.3233/bir-1968-5101. [DOI] [PubMed] [Google Scholar]