Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1970 Feb;206(2):243–256. doi: 10.1113/jphysiol.1970.sp009010

The effects of hypophysial portal plasma on the content of immunoreactive growth hormone in the anterior pituitary

M G Buse, J D Fulmer, P C Kansal, W C Worthington
PMCID: PMC1348643  PMID: 5498472

Abstract

1. Blood plasma obtained from the hypophysial portal vessels of rats has been tested in assay animals for effects on the content of growth hormone in the anterior pituitary.

2. Growth hormone was estimated by radioimmuno-assay at 15 min, 30 min, 1 hr and 2 hr after intracarotid or intravenous infusion of portal plasma. There was a significant increase in growth hormone content at 15 min after infusion, but not at 30 min, 1 hr and 2 hr.

3. Insulin, cold stress, haemorrhage and treatment with growth hormone had no effect on growth hormone content of the pituitary under the conditions of application of these stimuli.

4. Growth hormone was measured in plasma obtained from the pituitary of control and insulin-treated animals. In control animals the amount of growth hormone in this plasma was higher than in base line samples and in the insulin-treated animals it was lower than in base line samples. A calculation based on the growth hormone content of blood obtained directly from the pituitary indicates a daily rate of synthesis and release of about 10 μg.

5. The hypothesis that portal plasma stimulated the synthesis of immuno-assayable growth hormone by the anterior pituitary is considered.

6. Discrepancies between our results using immuno-assay and those of others using bio-assay are discussed.

Full text

PDF
243

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Averill R. L., Salaman D. F., Worthington W. C. Thyrotrophin releasing factor in hypophyseal portal blood. Nature. 1966 Jul 9;211(5045):144–145. doi: 10.1038/211144a0. [DOI] [PubMed] [Google Scholar]
  2. Birge C. A., Peake G. T., Mariz I. K., Daughaday W. H. Radioimmunoassayable growth hormone in the rat pituitary gland: effects of age, sex and hormonal state. Endocrinology. 1967 Aug;81(2):195–204. doi: 10.1210/endo-81-2-195. [DOI] [PubMed] [Google Scholar]
  3. Fink G. Further studies on luteinizing hormone releasing factor (LRF) in hypophysial portal blood of ratsportal blood of rats. J Physiol. 1967 Jul;191(2):125P–126P. [PubMed] [Google Scholar]
  4. Fink G., Nallar R., Worthington W. C., Jr Demonstration of luteinizing hormone releasing factor in hypophysial portal blood of pro-oestrous & hypophysectomized rats. J Physiol. 1967 Jul;191(2):407–416. doi: 10.1113/jphysiol.1967.sp008258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fink G. Nature of luteinizing hormone releasing factor in hypophysial portal blood. Nature. 1967 Jul 8;215(5097):159–161. doi: 10.1038/215159a0. [DOI] [PubMed] [Google Scholar]
  6. GREENSPAN F. S., LI C. H. Bioassay of hypophyseal growth hormone; the tibia test. Endocrinology. 1949 Nov;45(5):455-63, illust. doi: 10.1210/endo-45-5-455. [DOI] [PubMed] [Google Scholar]
  7. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Krulich L., Dhariwal A. P., McCann S. M. Growth hormone-releasing activity of crude ovine hypothalamic extracts. Proc Soc Exp Biol Med. 1965 Oct;120(1):180–184. doi: 10.3181/00379727-120-30480. [DOI] [PubMed] [Google Scholar]
  9. Krulich L., McCann S. M. Effect of alterations in blood sugar on pituitary growth hormone content in the rat. Endocrinology. 1966 Apr;78(4):759–764. doi: 10.1210/endo-78-4-759. [DOI] [PubMed] [Google Scholar]
  10. Krulich L., McCann S. M. Influence of growth hormone (GH) on content of GH in the pituitaries of normal rats. Proc Soc Exp Biol Med. 1966 Apr;121(4):1114–1117. doi: 10.3181/00379727-121-30980. [DOI] [PubMed] [Google Scholar]
  11. Müller E. E., Arimura A., Saito T., Schally A. V. Growth hormone-releasing activity in plasma of normal and hypophysectomized rats. Endocrinology. 1967 Jan;80(1):77–81. doi: 10.1210/endo-80-1-77. [DOI] [PubMed] [Google Scholar]
  12. Müller E. E., Saito T., Arimura A., Schally A. V. Hypoglycemia, stress and growth hormone release: blockade of growth hormone release by drugs acting on the central nervous system. Endocrinology. 1967 Jan;80(1):109–117. doi: 10.1210/endo-80-1-109. [DOI] [PubMed] [Google Scholar]
  13. Müller E., Pecile A. Influence of exogenous growth hormone on endogenous growth hormone release. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1289–1291. doi: 10.3181/00379727-122-31384. [DOI] [PubMed] [Google Scholar]
  14. PARKER M. L., JARETT L., SCHALCH D. S., KIPNIS D. M. RAT GROWTH HORMONE: IMMUNOFLUORESCENT AND RADIOIMMUNOLOGIC STUDIES. Endocrinology. 1965 May;76:928–932. doi: 10.1210/endo-76-5-928. [DOI] [PubMed] [Google Scholar]
  15. PORTER J. C., JONES J. C. Effect of plasma from hypophyseal-portal vessel blood on adrenal ascorbic acid. Endocrinology. 1956 Jan;58(1):62–67. doi: 10.1210/endo-58-1-62. [DOI] [PubMed] [Google Scholar]
  16. PORTER J. C., RUMSFELD H. W., Jr Effect of lyophilized plasma and plasma fractions from hypophyseal-portal vessel blood on adrenal ascorbic acid. Endocrinology. 1956 Mar;58(3):359–364. doi: 10.1210/endo-58-3-359. [DOI] [PubMed] [Google Scholar]
  17. Pecile A., Müller E., Falconi G., Martini L. Growth hormone-releasing activity of hypothalamic extracts at different ages. Endocrinology. 1965 Aug;77(2):241–246. doi: 10.1210/endo-77-2-241. [DOI] [PubMed] [Google Scholar]
  18. SCHALLY A. V., STEELMAN S. L., BOWERS C. Y. EFFECT OF HYPOTHALAMIC EXTRACTS ON RELEASE OF GROWTH HORMONE IN VITRO. Proc Soc Exp Biol Med. 1965 May;119:208–212. doi: 10.3181/00379727-119-30138. [DOI] [PubMed] [Google Scholar]
  19. Worthington W. C. Blood samples from the pituitary stalk of the rat: method of collection and factors determining volume. Nature. 1966 May 14;210(5037):710–712. doi: 10.1038/210710a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES