Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1970 Apr;207(2):329–350. doi: 10.1113/jphysiol.1970.sp009064

The secretory pattern of three stomach preparations in the rat

S E Svensson
PMCID: PMC1348709  PMID: 5499022

Abstract

1. In conscious rats provided with a gastric fistula, or a Pavlov pouch, or a Heidenhain pouch, the secretion of acid and pepsin was studied in the interdigestive state and in response to hog gastrin II. In Pavlov pouches and in Heidenhain pouches the responses to 2-deoxy-D-glucose and to food were established, as were also the responses to gastrin pentapeptide and methacholine in Heidenhain pouches.

2. Interdigestive secretion of acid and pepsin is substantial in the gastric fistula and the Pavlov pouch preparation, whereas in the Heidenhain pouch acid secretion is low or scanty, and the ratio pepsin/acid output is higher than in the innervated preparations.

3. Feeding evoked secretion of acid and pepsin in the Heidenhain and Pavlov pouches; the increase in pepsin secretion lasted much longer in the Heidenhain than in the Pavlov pouch.

4. Methacholine, infused in a threshold dose, augmented the acid output in response to food in the Heidenhain pouch, but did not affect the pepsin output.

5. Gastrin II (hog) evoked graded acid responses in the three stomach preparations, and the innervated preparations were more sensitive to gastrin II than the denervated one, whereas stimulation of pepsin secretion was apparent only in the Heidenhain pouch.

6. Methacholine, on infusion, in the Heidenhain pouch, gave a maximal acid secretory response, greater than twice that obtained by gastrin, and produced strong stimulation of pepsin.

7. Methacholine, infused in a threshold dose, accentuated the acid secretory response to graded doses of gastrin.

8. 2-deoxy-D-glucose stimulated acid and pepsin secretion consistently in the Pavlov pouch, whereas in the Heidenhain pouch the response was scanty or absent.

9. A large dose of gastrin pentapeptide injected intravenously while a background secretion was maintained by gastrin II, produced in the Heidenhain pouch triphasic changes in the sequence: stimulation, inhibition, stimulation.

Full text

PDF
329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALPHIN R. S., LIN T. M. Preparation of chronic denervated gastric pouches in the rat. Am J Physiol. 1959 Aug;197:257–259. doi: 10.1152/ajplegacy.1959.197.2.257. [DOI] [PubMed] [Google Scholar]
  2. ALPHIN R. S., LIN T. M. Response of chronic denervated gastric pouches of rats to food and histamine. Am J Physiol. 1962 Oct;203:641–643. doi: 10.1152/ajplegacy.1962.203.4.641. [DOI] [PubMed] [Google Scholar]
  3. Barrett A. M., Raventós J., Siddall R. A. Influence of some anaesthetics on pharmacologically stimulated gastric secretion in the rat. Br J Pharmacol Chemother. 1966 Oct;28(1):51–63. doi: 10.1111/j.1476-5381.1966.tb01873.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bitsch V. A modification of the Hunt method for estimating peptic activity in gastric juice. Scand J Clin Lab Invest. 1966;18(3):357–358. doi: 10.3109/00365516609087209. [DOI] [PubMed] [Google Scholar]
  5. GHOSH M. N., SCHILD H. O. Continuous recording of acid gastric secretion in the rat. Br J Pharmacol Chemother. 1958 Mar;13(1):54–61. doi: 10.1111/j.1476-5381.1958.tb00190.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GREGORY R. A. Gastric secretory responses after portal venous ligation. J Physiol. 1958 Nov 10;144(1):123–137. doi: 10.1113/jphysiol.1958.sp006090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GREGORY R. A., TRACY H. J. Secretory responses of denervated gastric pouches. Am J Dig Dis. 1960 Apr;5:308–323. doi: 10.1007/BF02232352. [DOI] [PubMed] [Google Scholar]
  8. Gregory R. A. Recent advances in the physiology of gastrin. Proc R Soc Lond B Biol Sci. 1968 May 14;170(1018):81–88. doi: 10.1098/rspb.1968.0026. [DOI] [PubMed] [Google Scholar]
  9. HUNT J. N., SPURRELL W. R. The pattern of emptying of the human stomach. J Physiol. 1951 Apr;113(2-3):157–168. doi: 10.1113/jphysiol.1951.sp004562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirschowitz B. I., Sachs G. Vagal gastric secretory stimulation by 2-deoxy-D-glucose. Am J Physiol. 1965 Sep;209(3):452–460. doi: 10.1152/ajplegacy.1965.209.3.452. [DOI] [PubMed] [Google Scholar]
  11. Hunt J. N. A method for estimating peptic activity in gastric contents. Biochem J. 1948;42(1):104–109. doi: 10.1042/bj0420104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KIM K. S., SHORE P. A. MECHANISM OF ACTION OF RESERPINE AND INSULIN ON GASTRIC AMINES AND GASTRIC ACID SECRETION, AND THE EFFECT OF MONOAMINE OXIDASE INHIBITION. J Pharmacol Exp Ther. 1963 Sep;141:321–325. [PubMed] [Google Scholar]
  13. Kahlson G., Rosengren E., Svensson S. E. Mode of action of a gastric-secretion antagonist. Br J Pharmacol Chemother. 1968 Jul;33(3):493–500. doi: 10.1111/j.1476-5381.1968.tb00497.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LANE A., IVY A. C., IVY E. K. Response of the chronic gastric fistula rat to histamine. Am J Physiol. 1957 Aug;190(2):221–228. doi: 10.1152/ajplegacy.1957.190.2.221. [DOI] [PubMed] [Google Scholar]
  15. Rosengren E., Svensson S. E. The role of the antrum and the vagus nerve in the formation of gastric mucosal histamine. J Physiol. 1969 Nov;205(2):275–288. doi: 10.1113/jphysiol.1969.sp008964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHOFIELD B. The pattern of pepsin secretion in innervated and in Heidenhain gastric pouches in dogs. Gastroenterology. 1957 Nov;33(5):714–729. [PubMed] [Google Scholar]
  17. SHAY H., SUN D. C., GRUENSTEIN M. A quantitative method for measuring spontaneous gastric secretion in the rat. Gastroenterology. 1954 Jun;26(6):906–913. [PubMed] [Google Scholar]
  18. Svensson S. E. Gastric secretion in unanaesthetized Pavlov pouch rats. J Physiol. 1969 Feb;200(2):116P–118P. [PubMed] [Google Scholar]
  19. THORNTON G. H., CLIFTON J. A. Estimation of gastric hydrochloric acid secretion in rats by a test meal technique. Am J Physiol. 1959 Aug;197:263–268. doi: 10.1152/ajplegacy.1959.197.2.263. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES