Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1970 Jul;208(3):547–562.1. doi: 10.1113/jphysiol.1970.sp009136

The recovery of resting potential and input resistance in sheep heart injured by knife or laser

J Délèze
PMCID: PMC1348786  PMID: 5503279

Abstract

1. A lesion 100 μ in diameter with well-defined boundaries was made with a laser in Purkinje fibres from sheep hearts. The membrane potential and the input resistance recorded in the intact tissue at about 0·5 mm from the edge of the lesion were found to drop at the instant of injury. The corresponding decrease of input resistance fits quantitatively the transmission line theory applied to a cable terminated by a short circuit at the lesion.

2. The input resistance and the membrane potential were found to rise simultaneously during healing-over. The membrane potential returned to its original level within 1 min. By then, the input resistance had settled either to the same value as before injury, or to a higher value matching quantitatively the theoretical resistance of a cable terminated by an infinite resistance at the lesion.

3. Neither membrane potential nor input resistance recovered in calcium-free solutions. But healing-over rapidly occurred when calcium was added to solutions that might otherwise differ widely in composition.

4. The transmission line model fits all observations if it is assumed that the injury causes a leak or short circuit in the `cable' that is soon closed, or rendered open circuit, by the development of a new diffusion barrier in the presence of calcium ions.

Full text

PDF
547

Images in this article

562-1Plate 1
on p.562-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRINK F. The role of calcium ions in neural processes. Pharmacol Rev. 1954 Sep;6(3):243–298. [PubMed] [Google Scholar]
  2. Boyle P. J., Conway E. J. Potassium accumulation in muscle and associated changes. J Physiol. 1941 Aug 11;100(1):1–63. doi: 10.1113/jphysiol.1941.sp003922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARMELIET E. E. Chloride ions and the membrane potential of Purkinje fibres. J Physiol. 1961 Apr;156:375–388. doi: 10.1113/jphysiol.1961.sp006682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DANIELSON B. G. THE DISTRIBUTION OF SOME ELECTROLYTES IN THE HEART. STUDIES ON NORMAL AND VAGUS-STIMULATED HEARTS. Acta Physiol Scand Suppl. 1964:SUPPL 236–236:1+. [PubMed] [Google Scholar]
  5. FATT P., KATZ B. An analysis of the end-plate potential recorded with an intracellular electrode. J Physiol. 1951 Nov 28;115(3):320–370. doi: 10.1113/jphysiol.1951.sp004675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fozzard H. A. Membrane capacity of the cardiac Purkinje fibre. J Physiol. 1966 Jan;182(2):255–267. doi: 10.1113/jphysiol.1966.sp007823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUTTER O. F., NOBLE D. Rectifying properties of heart muscle. Nature. 1960 Nov 5;188:495–495. doi: 10.1038/188495a0. [DOI] [PubMed] [Google Scholar]
  8. LING G., GERARD R. W. The normal membrane potential of frog sartorius fibers. J Cell Physiol. 1949 Dec;34(3):383–396. doi: 10.1002/jcp.1030340304. [DOI] [PubMed] [Google Scholar]
  9. LOEWENSTEIN W. R., KANNO Y. STUDIES ON AN EPITHELIAL (GLAND) CELL JUNCTION. I. MODIFICATIONS OF SURFACE MEMBRANE PERMEABILITY. J Cell Biol. 1964 Sep;22:565–586. doi: 10.1083/jcb.22.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Loewenstein W. R., Nakas M., Socolar S. J. Junctional membrane uncoupling. Permeability transformations at a cell membrane junction. J Gen Physiol. 1967 Aug;50(7):1865–1891. doi: 10.1085/jgp.50.7.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PAGE E., SOLOMON A. K. Cat heart muscle in vitro. I. Cell volumes and intracellular concentrations in papillary muscle. J Gen Physiol. 1960 Nov;44:327–344. doi: 10.1085/jgp.44.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PORTZEHL H., CALDWELL P. C., RUEEGG J. C. THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS. Biochim Biophys Acta. 1964 May 25;79:581–591. doi: 10.1016/0926-6577(64)90224-4. [DOI] [PubMed] [Google Scholar]
  13. SJOSTRAND F. S., ANDERSSON E. Electron microscopy of the intercalated discs of cardiac muscle tissue. Experientia. 1954 Sep 15;10(9):369–370. doi: 10.1007/BF02160542. [DOI] [PubMed] [Google Scholar]
  14. TARR M., SPERELAKIS N. WEAK ELECTROTONIC INTERACTION BETWEEN CONTIGUOUS CARDIAC CELLS. Am J Physiol. 1964 Sep;207:691–700. doi: 10.1152/ajplegacy.1964.207.3.691. [DOI] [PubMed] [Google Scholar]
  15. UEDA G. Cardiac membrane potentials recorded from the injured ventricular cells of dogs. Jpn J Physiol. 1959 Sep 15;9:375–386. doi: 10.2170/jjphysiol.9.375. [DOI] [PubMed] [Google Scholar]
  16. WEIDMANN S. Effect of current flow on the membrane potential of cardiac muscle. J Physiol. 1951 Oct 29;115(2):227–236. doi: 10.1113/jphysiol.1951.sp004667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WEIDMANN S. The electrical constants of Purkinje fibres. J Physiol. 1952 Nov;118(3):348–360. doi: 10.1113/jphysiol.1952.sp004799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weidmann S. The diffusion of radiopotassium across intercalated disks of mammalian cardiac muscle. J Physiol. 1966 Nov;187(2):323–342. doi: 10.1113/jphysiol.1966.sp008092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de Mello W. C., Motta G. E., Chapeau M. A study on the healing-over of myocardial cells of toads. Circ Res. 1969 Mar;24(3):475–487. doi: 10.1161/01.res.24.3.475. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES