Abstract
1. The responses of the cutaneous blood vessels of the guinea-pig to histamine and bradykinin have been studied in order to determine whether these factors mediate vascular changes by acting on different blood vessel receptors.
2. In animals injected intravenously with bovine serum albumin labelled with phosphorus 32P the reactions to histamine and bradykinin have been studied by continuously recording the local radioactive emission from the skin.
3. Histamine increased the intensity of radioactive emission for 7-15 min. The level of radioactivity then remained almost constant for at least 1 hr. Bradykinin increased the rate of emission for 5-7 min but the radioactivity decreased by approx. 30% in 1 hr.
4. The intensity of the response to histamine was markedly diminished in sites previously treated with either histamine or bradykinin, compared with the reaction in normal skin. However, a response of similar intensity to that in normal skin was evoked by histamine earlier in sites previously treated with bradykinin than in sites treated with histamine. By contrast, bradykinin evoked a similar reaction to that in normal skin, in sites previously treated with either histamine or bradykinin.
5. The dissimilarity of the responses evoked by histamine and bradykinin suggests that there are two types of blood vessel receptors. One type may be activated by both histamine and bradykinin but becomes refractory to further stimulation with histamine. The other type of receptor is activated only by bradykinin and does not become refractory.
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Selected References
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- Baumgarten A., Melrose G. J., Vagg W. J. Continuous quantitative recording of changes in vascular permeability. Experientia. 1967 Oct 15;23(10):884–885. doi: 10.1007/BF02146911. [DOI] [PubMed] [Google Scholar]
- Baumgarten A., Melrose G. J., Vagg W. J. Preparation of P32-phosphanilic acid-bovine serum albumin tracer. Anal Biochem. 1968 Aug;24(2):243–248. doi: 10.1016/0003-2697(68)90177-2. [DOI] [PubMed] [Google Scholar]
- Baumgarten A., Wilhelm D. L. Vascular permeability responses in hypersensitivity. I. The tuberculin reaction. Pathology. 1969 Oct;1(4):301–315. doi: 10.3109/00313026909071310. [DOI] [PubMed] [Google Scholar]
- CARR J., WILHELM D. L. THE EVALUATION OF INCREASED VASCULAR PERMEABILITY IN THE SKIN OF GUINEA-PIGS. Aust J Exp Biol Med Sci. 1964 Aug;42:511–522. doi: 10.1038/icb.1964.48. [DOI] [PubMed] [Google Scholar]
- Greaves M., Shuster S. Responses of skin blood vessels to bradykinin, histamine and 5-hydroxytryptamine. J Physiol. 1967 Nov;193(2):255–267. doi: 10.1113/jphysiol.1967.sp008356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILES A. A. LARGE MOLECULAR SUBSTANCES AS MEDIATORS OF THE INFLAMMATORY REACTION. Ann N Y Acad Sci. 1964 Aug 27;116:855–865. doi: 10.1111/j.1749-6632.1964.tb52551.x. [DOI] [PubMed] [Google Scholar]
- MILES A. A., MILES E. M. Vascular reactions to histamine, histamine-liberator and leukotaxine in the skin of guinea-pigs. J Physiol. 1952 Oct;118(2):228–257. doi: 10.1113/jphysiol.1952.sp004789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Majno G., Gilmore V., Leventhal M. On the mechanism of vascular leakage caused by histaminetype mediators. A microscopic study in vivo. Circ Res. 1967 Dec;21(6):833–847. doi: 10.1161/01.res.21.6.833. [DOI] [PubMed] [Google Scholar]
- Oyvin I. A., Gaponiuk P. Y., Oyvin V. I. The effect of bradykinin on permeability of skin blood vessels. Experientia. 1967 Nov 15;23(11):925–926. doi: 10.1007/BF02136224. [DOI] [PubMed] [Google Scholar]