Table 2. Parameters of M refinement rounds.

	Geometry		Tilt series		CTF
M refinement	Image warp grid	Particle poses	Stage angles	Volume warp grid	Defocus	Tilt series acquired
Round 1	3 × 3	✓	✓	3 × 3 × 2 × 10	-	-
Round 2	6 × 6	✓	✓	6 × 6 × 8 ×10	-	-
Round 3	6 × 6	✓	✓	6 × 6 × 8 × 10	✓	✓
Round 4	10 × 10	✓	✓	10 × 10 × 10 × 10	✓	✓