Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1973 May;231(1):105–115. doi: 10.1113/jphysiol.1973.sp010222

The effect of salt adaptation on the permeability and cation selectivity of the goldfish intestinal epithelium

J C Ellory, Jacqueline Nibelle, M W Smith
PMCID: PMC1350439  PMID: 4715338

Abstract

1. The short-term uptake of Na by the goldfish mucosa was compared using both inulin and choline as markers of extracellular space. The results were virtually identical, the distribution of both choline and inulin increasing rapidly to measure a space at 1 min which then remained nearly constant during a following 4 min incubation.

2. Using inulin as space marker, the uptake of various alkali metal cations was determined from a 1 min contact with the mucosa. The relative rates of uptake were Tl > K > Rb > Cs > Na > Li, with a low selectivity ratio, the range of permeabilities being no greater than 5.

3. The selectivity sequence was the same in both salt and fresh-water adapted fish. Of the alkali metal cations tested, only Na showed a significantly decreased uptake on adaptation to salt.

4. Isolated intestinal preparations from salt-adapted fish showed a reduced short-circuit current compared with fish adapted to fresh water, the values being 12·3 ± 1·2 and 35·7 ± 1·5 μA cm-2 respectively. In both cases the short-circuit current was equivalent to the net transport of Na measured isotopically.

5. In Krebs—Henseleit medium, the measured tissue resistance was approximately 100 Ω cm-2 for both salt and fresh-water adapted fish.

6. It is concluded that regulation of cation transport in goldfish intestinal epithelium is specific for Na and mediated primarily through cellular rather than extracellular pathways.

Full text

PDF
105

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Diamond J. M., Bossert W. H. Standing-gradient osmotic flow. A mechanism for coupling of water and solute transport in epithelia. J Gen Physiol. 1967 Sep;50(8):2061–2083. doi: 10.1085/jgp.50.8.2061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Diamond J. M., Wright E. M. Biological membranes: the physical basis of ion and nonelectrolyte selectivity. Annu Rev Physiol. 1969;31:581–646. doi: 10.1146/annurev.ph.31.030169.003053. [DOI] [PubMed] [Google Scholar]
  3. Ellory J. C., Evans M. H., Heal J. W., Smith M. W. Intracellular and transepithelial potential recording from the goldfish intestine. J Physiol. 1972 Oct;226(2):29P–30P. [PubMed] [Google Scholar]
  4. Ellory J. C., Lahlou B., Smith M. W. Changes in the intestinal transport of sodium induced by exposure of goldfish to a saline environment. J Physiol. 1972 Apr;222(2):497–509. doi: 10.1113/jphysiol.1972.sp009811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellory J. C., Lahlou B., Smith M. W. Intestinal transport of sodium by goldfish adapted to a saline environment. J Physiol. 1971 Jul;216(1):46P–47P. [PubMed] [Google Scholar]
  6. Frizzell R. A., Schultz S. G. Ionic conductances of extracellular shunt pathway in rabbit ileum. Influence of shunt on transmural sodium transport and electrical potential differences. J Gen Physiol. 1972 Mar;59(3):318–346. doi: 10.1085/jgp.59.3.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frömter E. The route of passive ion movement through the epithelium of Necturus gallbladder. J Membr Biol. 1972;8(3):259–301. doi: 10.1007/BF01868106. [DOI] [PubMed] [Google Scholar]
  8. Lahlou B., Henderson I. W., Sawyer W. H. Sodium exchanges in goldfish (Carassius auratus L.) adapted to a hypertonic saline solution. Comp Biochem Physiol. 1969 Mar;28(3):1427–1433. doi: 10.1016/0010-406x(69)90580-5. [DOI] [PubMed] [Google Scholar]
  9. Rose R. C., Schultz S. G. Studies on the electrical potential profile across rabbit ileum. Effects of sugars and amino acids on transmural and transmucosal electrical potential differences. J Gen Physiol. 1971 Jun;57(6):639–663. doi: 10.1085/jgp.57.6.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schultz S. G., Curran P. F., Chez R. A., Fuisz R. E. Alanine and sodium fluxes across mucosal border of rabbit ileum. J Gen Physiol. 1967 May;50(5):1241–1260. doi: 10.1085/jgp.50.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith M. W., Ellory J. C. Sodium-amino acid interactions in the intestinal epithelium. Philos Trans R Soc Lond B Biol Sci. 1971 Aug 20;262(842):131–140. doi: 10.1098/rstb.1971.0084. [DOI] [PubMed] [Google Scholar]
  12. Smith M. W. Sodium-glucose interactions in the goldfish intestine. J Physiol. 1966 Feb;182(3):559–573. doi: 10.1113/jphysiol.1966.sp007837. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES