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. 1969 Feb;200(2):387–401. doi: 10.1113/jphysiol.1969.sp008700

Distribution and kinetics of CoEDTA in smooth muscle, and its use as an extracellular marker

Alison F Brading, A W Jones
PMCID: PMC1350474  PMID: 4974401

Abstract

1. [60Co]EDTA has been evaluated as an extracellular marker in guinea-pig taenia coli and rabbit myometrium.

2. The complex was found to be stable, non-toxic, convenient to use and had the advantage of being readily measured without interfering with the analysis of water and electrolyte contents.

3. In both smooth muscles [60Co]EDTA distributed rapidly into a volume equivalent to that available to [14C]sorbitol and slightly greater than that available to inulin.

4. The optimum concentrations of carrier required to effectively saturate any adsorption sites were 0·1 mM-CoEDTA for taenia coli and 1·0 mM for myometrium.

5. [60Co]EDTA was distributed into a volume equivalent to the total water content of rabbit tendon, indicating that it was not excluded from connective tissue water.

6. About 5% of the [60Co]EDTA taken up by smooth muscle in 15 minutes exhibited slow efflux kinetics. This property was also exhibited by [14C]sorbitol and has been reported for other extracellular markers.

7. The efflux of 95% of [60Co]EDTA followed bulk diffusion kinetics for both taenia coli and myometrium.

8. The simultaneous efflux of 24Na indicated that 1-2% exchanged slowly while 98-99% of the 24Na exchange could be described by bulk diffusion kinetics, similar to [60Co]EDTA. About 15% of this 24Na was contained outside of the [60Co]EDTA space.

9. More detailed studies involving the analysis of simultaneous efflux of [60Co]EDTA and 24Na may help to locate and characterize the properties of the excess rapidly exchanging sodium (12-15 m-equiv/kg. wet wt.) in smooth muscle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARR L., MALVIN R. L. ESTIMATION OF EXTRACELLULAR SPACES OF SMOOTH MUSCLE USING DIFFERENT-SIZED MOLECULES. Am J Physiol. 1965 May;208:1042–1045. doi: 10.1152/ajplegacy.1965.208.5.1042. [DOI] [PubMed] [Google Scholar]
  2. BOZLER E., LAVINE D. Permeability of smooth muscle. Am J Physiol. 1958 Oct;195(1):45–49. doi: 10.1152/ajplegacy.1958.195.1.45. [DOI] [PubMed] [Google Scholar]
  3. BURNSTOCK G., DEWHURST D. J., SIMON S. E. Sodium exchange in smooth muscle. J Physiol. 1963 Jul;167:210–228. doi: 10.1113/jphysiol.1963.sp007142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BURNSTOCK G., HOLMAN M. E., PROSSER C. L. Electrophysiology of smooth muscle. Physiol Rev. 1963 Jul;43:482–527. doi: 10.1152/physrev.1963.43.3.482. [DOI] [PubMed] [Google Scholar]
  5. Bozler E. Determination of extracellular space in amphibian muscle. J Gen Physiol. 1967 May;50(5):1459–1465. doi: 10.1085/jgp.50.5.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brading A. F. A constant flow apparatus for measuring radioactive ion effluxes from guinea-pig taenia coli. J Physiol. 1967 Sep;192(2):15P–16P. [PubMed] [Google Scholar]
  7. Brading A. F., Setekleiv J. The effect of hypo- and hypertonic solutions on volume and ion distribution of smooth muscle of guinea-pig taenia coli. J Physiol. 1968 Mar;195(1):107–118. doi: 10.1113/jphysiol.1968.sp008449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Buck B., Goodford P. J. The distribution of ions in the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Apr;183(3):551–569. doi: 10.1113/jphysiol.1966.sp007883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CASTEELS R., KURIYAMA H. MEMBRANE POTENTIAL AND IONIC CONTENT IN PREGNANT AND NON-PREGNANT RAT MYOMETRIUM. J Physiol. 1965 Mar;177:263–287. doi: 10.1113/jphysiol.1965.sp007591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GOODFORD P. J., HERMANSEN K. Sodium and potassium movements in the unstriated muscle of the guinea-pig taenia coli. J Physiol. 1961 Oct;158:426–448. doi: 10.1113/jphysiol.1961.sp006778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GOODFORD P. J., LUELLMANN H. The uptake of ethanesulphonate-35S ions by muscular tissue. J Physiol. 1962 Apr;161:54–61. doi: 10.1113/jphysiol.1962.sp006872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GOODFORD P. J. THE LOSS OF RADIOACTIVE 45-CALCIUM FROM THE SMOOTH MUSCLE OF THE GUINEA-PIG TAENIA COLI. J Physiol. 1965 Jan;176:180–190. doi: 10.1113/jphysiol.1965.sp007543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goodford P. J. An interaction between potassium and sodium in the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Sep;186(1):11–26. doi: 10.1113/jphysiol.1966.sp008017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Goodford P. J., Leach E. H. The extracellular space of the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Sep;186(1):1–10. doi: 10.1113/jphysiol.1966.sp008016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Goodford P. J. The calcium content of the smooth muscle of the guinea-pig taenia coli. J Physiol. 1967 Sep;192(1):145–157. doi: 10.1113/jphysiol.1967.sp008293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jones A. W. Influence of oestrogen and progesterone on electrolyte accumulation in the rabbit myometrium. J Physiol. 1968 Jul;197(1):19P–20P. [PubMed] [Google Scholar]
  17. MANERY J. F. Water and electrolyte metabolism. Physiol Rev. 1954 Apr;34(2):334–417. doi: 10.1152/physrev.1954.34.2.334. [DOI] [PubMed] [Google Scholar]
  18. PERSOFF D. A. A comparison of methods for measuring efflux of labelled potassium from contracting rabbit atria. J Physiol. 1960 Jul;152:354–366. doi: 10.1113/jphysiol.1960.sp006492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss G. B. Homogeneity of extracellular space measurement in smooth muscle. Am J Physiol. 1966 Apr;210(4):771–776. doi: 10.1152/ajplegacy.1966.210.4.771. [DOI] [PubMed] [Google Scholar]

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