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. 1973 Mar;229(3):733–749. doi: 10.1113/jphysiol.1973.sp010164

Uptake of calcium and magnesium by rat duodenal mucosa analysed by means of competing metals

J M O'Donnell, M W Smith
PMCID: PMC1350560  PMID: 4693681

Abstract

1. The short-term uptake of Ca and Mg by rat duodenal mucosa consisted of two parts, one apparently complete within 1 min (uptake I) and a second which increased linearly with time from 1 to 12 min (uptake II).

2. Uptake II for both Ca and Mg showed a curvilinear dependence on concentration. Uptake I was related linearly to concentration up to the highest concentration tested.

3. Mg caused a significant inhibition of Ca uptake II. Ca was without effect on Mg uptake.

4. Mn reduced significantly both uptakes I and II for Ca; Ba and Sr were without effect on Ca uptake.

5. Two lanthanides, La and Pr, both inhibited uptakes I and II for Ca. Inhibition by Pr could be reversed by increasing the concentration of Ca. Some uptake II for Ca still persisted when uptake I was completely abolished by 3 mM-La.

6. It is suggested that a small part of Ca uptake I may be associated with the subsequent mediated entry into the mucosa, since inhibition of Ca uptake consistently involves both forms of uptake. However, since some Ca still enters the mucosa when uptake I is completely inhibited, it appears that some entry of Ca can occur by diffusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALCOCK N., MACINTYRE I. Inter-relation of calcium and magnesium absorption. Clin Sci. 1962 Apr;22:185–193. [PubMed] [Google Scholar]
  2. Aldor T. A., Moore E. W. Magnesium absorption by everted sacs of rat intestine and colon. Gastroenterology. 1970 Nov;59(5):745–753. [PubMed] [Google Scholar]
  3. HENDRIX Z. J., ALCOCK N. W., ARCHIBALD R. M. COMPETITION BETWEEN CALCIUM, STRONTIUM, AND MAGNESIUM FOR ABSORPTION IN THE ISOLATED RAT INTESTINE. Clin Chem. 1963 Dec;12:734–744. [PubMed] [Google Scholar]
  4. Helbock H. J., Forte J. G., Saltman P. The mechanism of calcium transport by rat intestine. Biochim Biophys Acta. 1966 Sep 5;126(1):81–93. doi: 10.1016/0926-6585(66)90039-2. [DOI] [PubMed] [Google Scholar]
  5. Martin D. L., Deluca H. F. Calcium transport and the role of vitamin D. Arch Biochem Biophys. 1969 Oct;134(1):139–148. doi: 10.1016/0003-9861(69)90260-4. [DOI] [PubMed] [Google Scholar]
  6. Meiri U., Rahamimoff R. Neuromuscular transmission: inhibition by manganese ions. Science. 1972 Apr 21;176(4032):308–309. doi: 10.1126/science.176.4032.308. [DOI] [PubMed] [Google Scholar]
  7. Mela L. Reaction of lanthanides with mitochondrial membranes. Ann N Y Acad Sci. 1969 Oct 31;147(19):824–828. doi: 10.1111/j.1749-6632.1969.tb41290.x. [DOI] [PubMed] [Google Scholar]
  8. O'Donnell J. M., Smith M. W. Uptake of calcium and magnesium by the duodenum of the rat. J Physiol. 1972 Feb;221(1):16P–17P. [PubMed] [Google Scholar]
  9. Papworth D. G., Patrick G. The kinetics of influx of calcium and strontium into rat intestine in vitro. J Physiol. 1970 Nov;210(4):999–1020. doi: 10.1113/jphysiol.1970.sp009254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROSS D. B. In vitro studies on the transport of magnesium across the intestinal wall of the rat. J Physiol. 1962 Mar;160:417–428. doi: 10.1113/jphysiol.1962.sp006856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SCHACHTER D., DOWDLE E. B., SCHENKER H. Active transport of calcium by the small intestine of the rat. Am J Physiol. 1960 Feb;198:263–268. doi: 10.1152/ajplegacy.1960.198.2.263. [DOI] [PubMed] [Google Scholar]
  12. Sallee V. L., Wilson F. A., Dietschy J. M. Determination of unidirectional uptake rates for lipids across the intestinal brush border. J Lipid Res. 1972 Mar;13(2):184–192. [PubMed] [Google Scholar]
  13. Schachter D., Kowarski S., Finkelstein J. D., Ma R. I. Tissue concentration differences during active transport of calcium by intestine. Am J Physiol. 1966 Nov;211(5):1131–1136. doi: 10.1152/ajplegacy.1966.211.5.1131. [DOI] [PubMed] [Google Scholar]
  14. Schultz S. G., Curran P. F., Chez R. A., Fuisz R. E. Alanine and sodium fluxes across mucosal border of rabbit ileum. J Gen Physiol. 1967 May;50(5):1241–1260. doi: 10.1085/jgp.50.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schultz S. G., Curran P. F. Coupled transport of sodium and organic solutes. Physiol Rev. 1970 Oct;50(4):637–718. doi: 10.1152/physrev.1970.50.4.637. [DOI] [PubMed] [Google Scholar]
  16. Walling M. W., Rothman S. S. Phosphate-independent, carrier-mediated active transport of calcium by rat intestine. Am J Physiol. 1969 Oct;217(4):1144–1148. doi: 10.1152/ajplegacy.1969.217.4.1144. [DOI] [PubMed] [Google Scholar]
  17. Wasserman R. H., Corradino R. A., Taylor A. N. Binding proteins from animals with possible transport function. J Gen Physiol. 1969 Jul 1;54(1):114–137. doi: 10.1085/jgp.54.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiss G. B., Goodman F. R. Effects of lanthanum on contraction, calcium distribution and Ca45 movements in intestinal smooth muscle. J Pharmacol Exp Ther. 1969 Sep;169(1):46–55. [PubMed] [Google Scholar]

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