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. 1973 Oct;234(2):241–255. doi: 10.1113/jphysiol.1973.sp010344

Protein fractions from mouse salivary glands affecting epithelial cells in organ culture

Barbara E C Banks, S J Walter
PMCID: PMC1350627  PMID: 4358349

Abstract

1. Extracts of male mouse submaxillary gland have been fractionated on G 150 Sephadex and DEAE cellulose.

2. A number of the protein fractions obtained appear to cause proliferation of epithelial cells in organ cultures of rat tissues.

3. All fractions active in organ culture possess proteolytic enzyme activity.

4. The proteolytic enzymes trypsin, pronase and collagenase produce epithelial disorganization in organ culture.

5. The organ culture effect is reduced in the presence of proteolytic enzyme inhibitors and abolished by pretreatment of proteins with diisopropylfluorophosphonate.

6. The presence of mitotic inhibitors does not reduce the organ culture effect.

7. The effect is not associated with an increase in the DNA content of tissue explants.

8. No evidence has been found for the existence, in mouse salivary glands, of a specific growth factor acting on epithelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angeletti P. U., Salvi M. L., Chesanow R. L., Cohen S. Azione dill' "epidermal growth factor" sulla sintesi de acidi nucleici e proteine dell'epitelio cutaneo. Experientia. 1964 Mar 15;20(3):146–148. doi: 10.1007/BF02150704. [DOI] [PubMed] [Google Scholar]
  2. Banks B. E., Walter S. J. Epithelial growth factor--an artifact? J Physiol. 1972 Sep;225(2):37P–38P. [PubMed] [Google Scholar]
  3. CERIOTTI G. A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952 Sep;198(1):297–303. [PubMed] [Google Scholar]
  4. COHEN S., ELLIOTT G. A. The stimulation of epidermal keratinization by a protein isolated from the submaxillary gland of the mouse. J Invest Dermatol. 1963 Jan;40:1–5. doi: 10.1038/jid.1963.1. [DOI] [PubMed] [Google Scholar]
  5. COHEN S. ISOLATION AND BIOLOGICAL EFFECTS OF AN EPIDERMAL GROWTH-STIMULATING PROTEIN. Natl Cancer Inst Monogr. 1964 Apr;13:13–37. [PubMed] [Google Scholar]
  6. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  7. Cohen S. The stimulation of epidermal proliferation by a specific protein (EGF). Dev Biol. 1965 Dec;12(3):394–407. doi: 10.1016/0012-1606(65)90005-9. [DOI] [PubMed] [Google Scholar]
  8. Curtis A. S. On the occurrence of specific adhesion between cells. J Embryol Exp Morphol. 1970 Feb;23(1):253–272. [PubMed] [Google Scholar]
  9. Curtis A. S., Van de Vyver G. The control of cell adhesion in a morphogenetic system. J Embryol Exp Morphol. 1971 Oct;26(2):295–312. [PubMed] [Google Scholar]
  10. Greene L. A., Shooter E. M., Varon S. Enzymatic activities of mouse nerve growth factor and its subunits. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1383–1388. doi: 10.1073/pnas.60.4.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones R. O., Ashwood-Smith M. J. Some preliminary observations on the biochemical and biological properties of an epithelial growth factor. Exp Cell Res. 1970 Jan;59(1):161–163. doi: 10.1016/0014-4827(70)90636-1. [DOI] [PubMed] [Google Scholar]
  12. Jones R. O. The in vitro effect of epithelial growth factor on rat organ cultures. Exp Cell Res. 1966 Oct;43(3):645–656. doi: 10.1016/0014-4827(66)90036-x. [DOI] [PubMed] [Google Scholar]
  13. Kuroda Y. Preparation of an aggregation-promoting supernatant from embryonic chick liver cells. Exp Cell Res. 1968 Mar;49(3):626–637. doi: 10.1016/0014-4827(68)90209-7. [DOI] [PubMed] [Google Scholar]
  14. LASNITZKI I. THE EFFECT OF METHYLCHOLANTHRENE ON RAT PROSTATE GLANDS GROWN IN NATURAL AND SEMI-DEFINED MEDIUM. Cancer Res. 1964 Jul;24:973–982. [PubMed] [Google Scholar]
  15. Moscona A. A. Cell aggregation: properties of specific cell-ligands and their role in the formation of multicellular systems. Dev Biol. 1968 Sep;18(3):250–277. doi: 10.1016/0012-1606(68)90035-3. [DOI] [PubMed] [Google Scholar]
  16. TROWELL O. A. The culture of mature organs in a synthetic medium. Exp Cell Res. 1959 Jan;16(1):118–147. doi: 10.1016/0014-4827(59)90201-0. [DOI] [PubMed] [Google Scholar]
  17. Wallis C., Ver B., Melnick J. L. The role of serum and fetuin in the growth of monkey kidney cells in culture. Exp Cell Res. 1969 Dec;58(2):271–282. doi: 10.1016/0014-4827(69)90505-9. [DOI] [PubMed] [Google Scholar]

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