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. 1973 Oct;234(1):127–143. doi: 10.1113/jphysiol.1973.sp010338

Sodium-dependent transport of sugars and iodide from the cerebral ventricles of the rabbit

M W B Bradbury, Hans E Brøndsted
PMCID: PMC1350655  PMID: 4766217

Abstract

1. The objective was to discover whether the extraction of sugars and iodide from the perfused cerebral ventricles is Na+-dependent.

2. In the ventriculo-aqueductal and ventriculo-cisternal perfusion systems in the rabbit the extraction of 14C-labelled D-hexoses (glucose, 3-O-methyl-glucose, α-methyl-glucoside and galactose), 131I- and 24Na was inhibited when 82% of the Na+ in the perfusion fluid was replaced by choline. The extraction returned to control levels when the Na+ concentration in the perfusion fluid was returned to normal.

3. Ouabain, 5 × 10-5 M in the perfusion fluid inhibited the extraction of the above 14C sugars and 131I-, but hardly affected that of [3H]2-deoxy-D-glucose. It enhanced the extraction of 24Na. C.s.f. production was usually totally inhibited.

4. The extraction of [14C]urea remained unchanged during perfusion with low Na+ fluid or ouabain.

5. Recovery from brain of [14C]3-O-methyl-glucose, [3H]2-deoxy-glucose and 131I- was low while recovery of [14C]α-methyl-glucoside and 24Na was high. On an equal weight basis recovery of [14C]3-O-methyl-glucose was about twelve times higher from the choroid plexus than from the brain.

6. Part of the movement of 14C sugars may be explained on basis of a Na+-gradient hypothesis with involvement of the Na+ pump at the blood—c.s.f. or blood—brain barriers.

7. The rate of c.s.f. production from the first three ventricles comprised about 40% of the rate from all four ventricles. The extraction of sugars, urea and cations was similar in both perfusion systems while the extraction of 131I- was higher in the ventriculo-cisternal system than in the ventriculo-aqueductal system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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