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. 1973 Dec;235(3):625–637. doi: 10.1113/jphysiol.1973.sp010408

Effect of cations on the blood platelet release reaction

J M Sneddon, K I Williams
PMCID: PMC1350784  PMID: 4203983

Abstract

1. Stimulus-secretion phenomena in blood platelets incubated with trypsin or thrombin have been investigated.

2. Secretion occurs in at least two stages: (a) induction, obtained when the platelets are incubated with proteolytic enzymes in the presence of the chelating agent EDTA, and (b) release, which occurs with the subsequent addition of Ca2+ to the incubation medium.

3. Ca2+ was the only essential divalent cation required for release. Ba2+ and Mg2+ inhibited release and Sr2+ could only partially substitute for Ca2+.

4. Blood platelets exhibited recovery from enzymic perturbation by trypsin or thrombin by a mechanism that was temperature dependent, but did not require the synthesis of new protein.

5. The results are discussed in relationship to stimulus—secretion in other cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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