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. 1974 Jan;236(2):465–472. doi: 10.1113/jphysiol.1974.sp010446

Levels of prostaglandins in human endometrium during the normal menstrual cycle

J Downie, N L Poyser, M Wunderlich
PMCID: PMC1350813  PMID: 16992446

Abstract

The levels of prostaglandins in the human endometrium throughout the normal menstrual cycle were measured by bioassay. The identity of prostaglandin F2α and prostaglandin E2 in endometrial tissue extracts was established by combined gas chromatography/mass spectrometry.

The levels of prostaglandin F2α and prostaglandin E2 are low during the proliferative phase of the cycle (10-25 ng/100 mg tissue). Prostaglandin F2α rises significantly during the luteal phase to levels of 65-75 ng/100 mg of tissue. Prostaglandin E2 levels are similar to prostaglandin F2α levels in the proliferative phase but remain lower during the luteal phase. The prostaglandin E2 level is highest at menstruation (52 ng/100 mg tissue). The implications of these findings are discussed in relation to the menstrual process and to the steroid hormonal changes during the cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN J. B., KELLAR R., MATTHEW G. D. Preliminary observations on urinary oestrogen excretion in certain gynaecological disorders. J Obstet Gynaecol Br Emp. 1959 Apr;66(2):177–211. doi: 10.1111/j.1471-0528.1959.tb01999.x. [DOI] [PubMed] [Google Scholar]
  2. Beavis E. L., Brown J. B., Smith M. A. Ovarian function after hysterectomy with conservation of the ovaries in pre-menopausal women. J Obstet Gynaecol Br Commonw. 1969 Nov;76(11):969–978. doi: 10.1111/j.1471-0528.1969.tb09462.x. [DOI] [PubMed] [Google Scholar]
  3. Blatchley F. R., Donovan B. T., Horton E. W., Poyser N. L. The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment. J Physiol. 1972 May;223(1):69–88. doi: 10.1113/jphysiol.1972.sp009835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHAMBERS P. L., PICKLES V. R. Plain-muscle stimulants in extracts of menstrual fluid and of endometrial curettings. J Physiol. 1958 Nov 10;144(1):68–79. doi: 10.1113/jphysiol.1958.sp006086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caldwell B. V., Tillson S. A., Brock W. A., Speroff L. The effects of exogenous progesterone and estradiol on prostaglandin F levels in ovariectomized ewes. Prostaglandins. 1972 Mar;1(3):217–228. doi: 10.1016/0090-6980(72)90006-8. [DOI] [PubMed] [Google Scholar]
  6. Csapo A. I., Pinto-Dantas C. R. The cyclic activity of the nonpregnant human uterus. A new method for recording intrauterine pressure. Fertil Steril. 1966 Jan-Feb;17(1):34–38. doi: 10.1016/s0015-0282(16)35823-x. [DOI] [PubMed] [Google Scholar]
  7. Cseply J., Csapo A. I. The effect of prostaglandin F2alpha on the small arteries of the omentum uteri in the rat. Prostaglandins. 1972 Mar;1(3):235–238. doi: 10.1016/0090-6980(72)90008-1. [DOI] [PubMed] [Google Scholar]
  8. EGLINTON G., RAPHAEL R. A., SMITH G. N., HALL W. J., PICKLES V. R. ISOLATION AND IDENTIFICATION OF TWO SMOOTH MUSCLE STIMULANTS FROM MENSTRUAL FLUID. Nature. 1963 Dec 7;200:960–PASSIM. doi: 10.1038/200960a0. [DOI] [PubMed] [Google Scholar]
  9. Fraser I. S., Baird D. T., Hobson B. M., Michie E. A., Hunter W. Cyclical ovarian function in women with congenital absence of the uterus and vagina. J Clin Endocrinol Metab. 1973 Apr;36(4):634–637. doi: 10.1210/jcem-36-4-634. [DOI] [PubMed] [Google Scholar]
  10. GREEN K., SAMUELSSON B. PROSTAGLANDINS AND RELATED FACTORS: XIX. THIN-LAYER CHROMATOGRAPHY OF PROSTAGLANDINS. J Lipid Res. 1964 Jan;5:117–120. [PubMed] [Google Scholar]
  11. PICKLES V. R. A plain-muscle stimulant in the menstruum. Nature. 1957 Nov 30;180(4596):1198–1199. doi: 10.1038/1801198a0. [DOI] [PubMed] [Google Scholar]
  12. PICKLES V. R., CLITHEROE H. J. Further studies of the menstrual stimulant. Lancet. 1960 Oct 29;2(7157):959–960. doi: 10.1016/s0140-6736(60)92029-8. [DOI] [PubMed] [Google Scholar]
  13. PICKLES V. R., HALL W. J., BEST F. A., SMITH G. N. PROSTAGLANDINS IN ENDOMETRIUM AND MENSTRUAL FLUID FROM NORMAL AND DYSMENORRHOEIC SUBJECTS. J Obstet Gynaecol Br Commonw. 1965 Apr;72:185–192. doi: 10.1111/j.1471-0528.1965.tb01415.x. [DOI] [PubMed] [Google Scholar]
  14. PICKLES V. R., HALL W. J. SOME PHYSIOLOGICAL PROPERTIES OF THE 'MENSTRUAL STIMULANT' SUBSTANCES A1 AND A2. J Reprod Fertil. 1963 Oct;6:315–317. doi: 10.1530/jrf.0.0060315. [DOI] [PubMed] [Google Scholar]
  15. Porter D. G., Behrman H. R. Prostaglandin-induced myometrial activity inhibited by progesterone. Nature. 1971 Aug 27;232(5313):627–628. doi: 10.1038/232627a0. [DOI] [PubMed] [Google Scholar]
  16. Poyser N. L. Production of prostaglandins by the guinea-pig uterus. J Endocrinol. 1972 Jul;54(1):147–159. doi: 10.1677/joe.0.0540147. [DOI] [PubMed] [Google Scholar]
  17. Thompson C. J., Los M., Horton E. W. The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry. Life Sci I. 1970 Sep 1;9(17):983–988. doi: 10.1016/0024-3205(70)90121-9. [DOI] [PubMed] [Google Scholar]
  18. VANE J. R. A sensitive method for the assay of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1957 Sep;12(3):344–349. doi: 10.1111/j.1476-5381.1957.tb00146.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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