Skip to main content
The Journal of Physiology logoLink to The Journal of Physiology
. 1969 May;202(1):211–221. doi: 10.1113/jphysiol.1969.sp008805

Chloride fluxes in crab muscle fibres

C D Richards
PMCID: PMC1351475  PMID: 5770889

Abstract

1. Isotopic techniques were used to study the efflux of Cl- from single cannulated muscle fibres of the crab Maia squinado.

2. The efflux of 36Cl- was found to be exponential with steady rate constants ranging from 0·01 to 0·07 min-1. The steady efflux was ca. 1000-2000 pM cm-2 sec-1. The efflux was slightly depressed by K+-free saline and was unaffected by 40 mM-K+ saline and NO3- saline. The influx and efflux of Cl- were presumed independent.

3. The membrane conductance calculated from 36Cl- flux data was consistent with that determined by the method of electrotonic spread for other marine crustacean muscle fibres. Cl- accounted for most of the membrane conductance.

Full text

PDF
211

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. Internal chloride concentration and chloride efflux of frog muscle. J Physiol. 1961 May;156:623–632. doi: 10.1113/jphysiol.1961.sp006698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRINLEY F. J., Jr SODIUM, POTASSIUM, AND CHLORIDE CONCENTRATIONS AND FLUXES IN THE ISOLATED GIANT AXON OF HOMARUS. J Neurophysiol. 1965 Jul;28:742–772. doi: 10.1152/jn.1965.28.4.742. [DOI] [PubMed] [Google Scholar]
  3. CALDWELL P. C., KEYNES R. D. The permeability of the squid giant axon to radioactive potassium and chloride ions. J Physiol. 1960 Nov;154:177–189. doi: 10.1113/jphysiol.1960.sp006572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CALDWELL P. C., WALSTER G. STUDIES ON THE MICRO-INJECTION OF VARIOUS SUBSTANCES INTO CRAB MUSCLE FIBRES. J Physiol. 1963 Nov;169:353–372. doi: 10.1113/jphysiol.1963.sp007261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DEMELLO W. C. ROLE OF CHLORIDE IONS IN CARDIAC ACTION AND PACEMAKER POTENTIALS. Am J Physiol. 1963 Sep;205:567–575. doi: 10.1152/ajplegacy.1963.205.3.567. [DOI] [PubMed] [Google Scholar]
  6. FATT P., GINSBORG B. L. The ionic requirements for the production of action potentials in crustacean muscle fibres. J Physiol. 1958 Aug 6;142(3):516–543. doi: 10.1113/jphysiol.1958.sp006034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FATT P., KATZ B. The electrical properties of crustacean muscle fibres. J Physiol. 1953 Apr 28;120(1-2):171–204. doi: 10.1113/jphysiol.1953.sp004884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIRARDIER L., REUBEN J. P., BRANDT P. W., GRUNDFEST H. EVIDENCE FOR ANION-PERMSELECTIVE MEMBRANE IN CRAYFISH MUSCLE FIBERS AND ITS POSSIBLE ROLE IN EXCITATION-CONTRACTION COUPLING. J Gen Physiol. 1963 Sep;47:189–214. doi: 10.1085/jgp.47.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HARRIS E. J. Anion interaction in frog muscle. J Physiol. 1958 Apr 30;141(2):351–365. doi: 10.1113/jphysiol.1958.sp005979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HODGKIN A. L., KEYNES R. D. Experiments on the injection of substances into squid giant axons by means of a microsyringe. J Physiol. 1956 Mar 28;131(3):592–616. doi: 10.1113/jphysiol.1956.sp005485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUTTER O. F., NOBLE D. The chloride conductance of frog skeletal muscle. J Physiol. 1960 Apr;151:89–102. [PMC free article] [PubMed] [Google Scholar]
  13. HUTTER O. F., PADSHA S. M. Effect of nitrate and other anions on the membrane resistance of frog skeletal muscle. J Physiol. 1959 Apr 23;146(1):117–132. doi: 10.1113/jphysiol.1959.sp006182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hall T. C., Cocking E. C. High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins. Biochem J. 1965 Sep;96(3):626–633. doi: 10.1042/bj0960626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KEYNES R. D. CHLORIDE IN THE SQUID GIANT AXON. J Physiol. 1963 Dec;169:690–705. doi: 10.1113/jphysiol.1963.sp007289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KEYNES R. D. The ionic fluxes in frog muscle. Proc R Soc Lond B Biol Sci. 1954 May 27;142(908):359–382. doi: 10.1098/rspb.1954.0030. [DOI] [PubMed] [Google Scholar]
  17. KEYNES R. D. The ionic movements during nervous activity. J Physiol. 1951 Jun;114(1-2):119–150. doi: 10.1113/jphysiol.1951.sp004608. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES