Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1969 Jun;202(2):329–338. doi: 10.1113/jphysiol.1969.sp008814

Factors affecting mucosal water and sodium transfer in everted sacs of rat jejunum

Ann D Crocker, Kenneth A Munday
PMCID: PMC1351485  PMID: 5784291

Abstract

1. Mucosal water and sodium transfer were measured in everted sacs of rat jejunum.

2. There was no effect on mucosal water and sodium transfer when aldosterone was added to the incubation medium.

3. An 87% increase in mucosal water and sodium transfer was obtained in sacs prepared from animals maintained on a low sodium diet compared to sacs from animals on a normal diet.

4. Injection of 5 μg aldosterone intraperitoneally to animals maintained on a high sodium diet produced a significant increase in mucosal water and sodium transfer in sacs prepared 27 hr later and this was still present 36 hr after injection.

5. The aldosterone-induced rise in mucosal water transfer was blocked if actinomycin D was administered one hour before the aldosterone injection.

6. It is suggested that aldosterone action on sodium and water transport in rat jejunum is mediated through a primary action on protein synthesis and that the long delay can be explained in terms of gut morphology.

Full text

PDF
329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARGER A. C., BERLIN R. D., TULENKO J. F. Infusion of aldosterone, 9-alpha-fluorohydrocortisone and antidiuretic hormone into the renal artery of normal and adrenalectomized, unanesthetized dogs: effect on electrolyte and water excretion. Endocrinology. 1958 Jun;62(6):804–815. doi: 10.1210/endo-62-6-804. [DOI] [PubMed] [Google Scholar]
  2. BERGER E. Y., KANZAKI G., STEELE J. M. The effect of deoxycorticosterone on the unidirectional transfers of sodium and potassium into and out of the dog intestine. J Physiol. 1960 May;151:352–362. doi: 10.1113/jphysiol.1960.sp006443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clarke A. M., Miller M., Shields R. Intestinal transport of sodium, potassium, and water in the dog during sodium depletion. Gastroenterology. 1967 May;52(5):846–858. [PubMed] [Google Scholar]
  4. EDELMAN I. S., BOGOROCH R., PORTER G. A. ON THE MECHANISM OF ACTION OF ALDOSTERONE ON SODIUM TRANSPORT: THE ROLE OF PROTEIN SYNTHESIS. Proc Natl Acad Sci U S A. 1963 Dec;50:1169–1177. doi: 10.1073/pnas.50.6.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edmonds C. J., Marriott J. C. The effect of aldosterone and adrenalectomy on the electrical potential difference of rat colon and on the transport of sodium, potassium, chloride and bicarbonate. J Endocrinol. 1967 Dec;39(4):517–531. doi: 10.1677/joe.0.0390517. [DOI] [PubMed] [Google Scholar]
  6. Fimognari G. M., Fanestil D. D., Edelman I. S. Induction of RNA and protein synthesis in the action of aldosterone in the rat. Am J Physiol. 1967 Oct;213(4):954–962. doi: 10.1152/ajplegacy.1967.213.4.954. [DOI] [PubMed] [Google Scholar]
  7. HARTROFT P. M., HARTROFT W. S. Studies on renal juxtaglomerular cells. I. Variations produced by sodium chloride and desoxycorticosterone acetate. J Exp Med. 1953 Mar;97(3):415–429. doi: 10.1084/jem.97.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LARAGH J. H. Aldosterone in fluid and electrolyte disorders: hyper- and hypoaldosteronism. J Chronic Dis. 1960 Mar;11:292–318. doi: 10.1016/0021-9681(60)90041-2. [DOI] [PubMed] [Google Scholar]
  9. LEVITAN R., INGELFINGER F. J. EFFECT OF D-ALDOSTERONE ON SALT AND WATER ABSORPTION FROM THE INTACT HUMAN COLON. J Clin Invest. 1965 May;44:801–808. doi: 10.1172/JCI105192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Norman A. W. Actinomycin D effect on lag in vitamin D-mediated calcium absorption in the chick. Am J Physiol. 1966 Sep;211(3):829–834. doi: 10.1152/ajplegacy.1966.211.3.829. [DOI] [PubMed] [Google Scholar]
  11. POUTSIAKA J. W., THOMAS B. G., LINEGAR C. R. Effect of fluorohydrocortisone on gastrointestinal and renal excretion of cations by the dog. Proc Soc Exp Biol Med. 1957 Dec;96(3):712–714. doi: 10.3181/00379727-96-23585. [DOI] [PubMed] [Google Scholar]
  12. Shields R., Mulholland A. T., Elmslie R. G. Action of aldosterone upon the intestinal transport of potassium, sodium, and water. Gut. 1966 Dec;7(6):686–696. doi: 10.1136/gut.7.6.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sonnenblick E. H., Cannon P. J., Laragh J. H. THE NATURE OF THE ACTION OF INTRAVENOUS ALDOSTERONE: EVIDENCE FOR A ROLE OF THE HORMONE IN URINARY DILUTION. J Clin Invest. 1961 Jun;40(6):903–913. doi: 10.1172/JCI104329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WIDNER W. R., STORER J. B., LUSHBAUGH C. C. The use of x-ray and nitrogen mustard to determine the mitotic and intermitotic times in normal and malignant rat tissues. Cancer Res. 1951 Nov;11(11):877–884. [PubMed] [Google Scholar]
  15. WILLIAMSON H. E. MECHANISM OF THE ANTINATRIURETIC ACTION OF ALDOSTERONE. Biochem Pharmacol. 1963 Dec;12:1449–1450. doi: 10.1016/0006-2952(63)90220-x. [DOI] [PubMed] [Google Scholar]
  16. WILSON T. H., WISEMAN G. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J Physiol. 1954 Jan;123(1):116–125. doi: 10.1113/jphysiol.1954.sp005036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WRONG O., MORRISON R. B., HURST P. E. A method of obtaining faecal fluid by in-vivo dialysis. Lancet. 1961 Jun 3;1(7188):1208–1209. doi: 10.1016/s0140-6736(61)91947-x. [DOI] [PubMed] [Google Scholar]
  18. Zull J. E., Czarnowska-Misztal E., Deluca H. F. Actinomycin D Inhibition of Vitamin D Action. Science. 1965 Jul 9;149(3680):182–184. doi: 10.1126/science.149.3680.182. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES