Abstract
1. A technique is described for the continuous assessment of acid secretion by the guinea-pig gastric mucosa at selected levels of intragastric pH.
2. The rate of hydrogen ion secretion increases linearly with pH over the intragastric range of pH 4·4 to 9·4. Sodium and potassium secretions are not clearly related to pH. Outputs of both chloride and bicarbonate rise as pH is increased.
3. The carbonic anhydrase inhibitor acetazolamide reversibly reduces rapid rates of acid secretion as occur when gastric contents are maintained at pH 10.
4. Alkali stimulated acid output is not inhibited by atropine (2 mg/kg) and probably results directly from increased carbonic anhydrase activity as the in vitro activity of the enzyme may be correlated with the rate of acid secretion over a wide range of pH.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AARSEN P. N., van NOORDWIJK J. The effect of atropine, propantheline and poldine on the vagally stimulated gastric motility and the histamine-stimulated acid gastric secretion in the rat. Br J Pharmacol Chemother. 1961 Aug;17:41–50. doi: 10.1111/j.1476-5381.1961.tb01102.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berkowitz J. M., Janowitz H. D. Secretion of sodium by the resting, stimulated, and inhibited canine gastric mucosa. Am J Physiol. 1967 Jan;212(1):72–76. doi: 10.1152/ajplegacy.1967.212.1.72. [DOI] [PubMed] [Google Scholar]
- CODE C. F., HIGGINS J. A., MOLL J. C., ORVIS A. L., SCHOLER J. F. The influence of acid on the gastric absorption of water, sodium and potassium. J Physiol. 1963 Apr;166:110–119. doi: 10.1113/jphysiol.1963.sp007093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies R. E. Hydrochloric acid production by isolated gastric mucosa: With an appendix by R. E. Davies and F. J. W. Roughton. Biochem J. 1948;42(4):609–621. [PMC free article] [PubMed] [Google Scholar]
- Jacobson E. D., Swan K. G., Grossman M. I. Blood flow and secretion in the stomach. Gastroenterology. 1967 Feb;52(2):414–420. [PubMed] [Google Scholar]
- LINDNER A. E. ELECTROLYTE CHANGES IN RAT STOMACHS FOLLOWING INSTILLATION OF ACID SOLUTIONS. Am J Physiol. 1964 Sep;207:606–612. doi: 10.1152/ajplegacy.1964.207.3.606. [DOI] [PubMed] [Google Scholar]
- Lindner A. E. Effect of histamine on electrolyte changes in solutions instilled in rat stomachs. Am J Physiol. 1966 Jun;210(6):1321–1326. doi: 10.1152/ajplegacy.1966.210.6.1321. [DOI] [PubMed] [Google Scholar]
- MOLL J. C., CODE C. F. Rates of insorption of sodium and potassium from stomachs of rats. Am J Physiol. 1962 Aug;203:229–231. doi: 10.1152/ajplegacy.1962.203.2.229. [DOI] [PubMed] [Google Scholar]
- Makhlouf G. M., McManus J. P., Card W. I. A quantitative statement of the two-component hypothesis of gastric secretion. Gastroenterology. 1966 Aug;51(2):149–171. [PubMed] [Google Scholar]
- Moody F. G. Gastric blood flow and acid secretion during direct intraarterial histamine administration. Gastroenterology. 1967 Feb;52(2):216–224. [PubMed] [Google Scholar]
- POLLER L. The effect of acetazoleamide, an inhibitor of carbonic anhydrase, on gastric secretion. Br J Pharmacol Chemother. 1956 Sep;11(3):263–266. doi: 10.1111/j.1476-5381.1956.tb01064.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL D. W., ROBBINS R. C., BOYETT J. D., HIRSCHOWITZ B. I. Evaluation of the gastric Na:H exchange mechanism using histamine and diamox. Am J Physiol. 1962 Feb;202:293–301. doi: 10.1152/ajplegacy.1962.202.2.293. [DOI] [PubMed] [Google Scholar]
- Roughton F. J., Booth V. H. The effect of substrate concentration, pH and other factors upon the activity of carbonic anhydrase. Biochem J. 1946;40(2):319–330. doi: 10.1042/bj0400319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voith K., Kovacs B. A. Differences in gastric acid output in response to histamine between male and female guinea pigs. Can J Physiol Pharmacol. 1966 Jul;44(4):515–520. doi: 10.1139/y66-066. [DOI] [PubMed] [Google Scholar]