Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1968 Jun;196(3):703–711. doi: 10.1113/jphysiol.1968.sp008531

Action of amphotericin B on the toad bladder: evidence for sodium transport along two pathways

P J Bentley
PMCID: PMC1351772  PMID: 5664238

Abstract

1. The relationship of short-circuit current (SCC, sodium transport) and potential difference (p.d.) across the toad bladder to the concentration of sodium on the mucosal side was measured. Maximal values were attained when the Na concentration was 10 mM for SCC and 30 mM for p.d.

2. Amphotericin B increased SCC across bladders bathed on their mucosal surface with isotonic (115 mM) NaCl solution and this effect was not inhibited by cyanide and iodoacetate. The osmotic permeability of the bladder was also increased by amphotericin B.

3. Vasopressin and aldosterone increased sodium transport from solutions of low Na concentration, but amphotericin B was ineffective until a level of about 40-60 mM was present.

4. The evidence suggests two pathways for sodium transport across the bladder distinguished by differences in their electrochemical gradients with the fluid at the mucosal side.

Full text

PDF
703

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENTLEY P. J. The effects of neurohypophysial extracts on the water transfer across the wall of the isolated urinary bladder of the toad Bufo marinus. J Endocrinol. 1958 Sep;17(3):201–209. doi: 10.1677/joe.0.0170201. [DOI] [PubMed] [Google Scholar]
  2. Biber T. U., Chez R. A., Curran P. F. Na transport across frog skin at low external Na concentrations. J Gen Physiol. 1966 Jul;49(6):1161–1176. doi: 10.1085/jgp.0491161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRABBE J. Stimulation of active sodium transport by the isolated toad bladder with aldosterone in vitro. J Clin Invest. 1961 Nov;40:2103–2110. doi: 10.1172/JCI104436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cereijido M., Rotunno C. A. Transport and distribution of sodium across frog skin. J Physiol. 1967 Jun;190(3):481–497. doi: 10.1113/jphysiol.1967.sp008223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crabbé J., De Weer P. Action of aldosterone and vasopressin on the active transport of sodium by the isolated toad bladder. J Physiol. 1965 Oct;180(3):560–568. doi: 10.1113/jphysiol.1965.sp007717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ENGBAEK L., HOSHIKO T. Electrical potential gradients through frog skin. Acta Physiol Scand. 1957 Jul 1;39(4):348–355. doi: 10.1111/j.1748-1716.1957.tb01433.x. [DOI] [PubMed] [Google Scholar]
  7. FRAZIER H. S. The electrical potential profile of the isolated toad bladder. J Gen Physiol. 1962 Jan;45:515–528. doi: 10.1085/jgp.45.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  9. LEAF A., ANDERSON J., PAGE L. B. Active sodium transport by the isolated toad bladder. J Gen Physiol. 1958 Mar 20;41(4):657–668. doi: 10.1085/jgp.41.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LICHTENSTEIN N. S., LEAF A. EFFECT OF AMPHOTERICIN B ON THE PERMEABILITY OF THE TOAD BLADDER. J Clin Invest. 1965 Aug;44:1328–1342. doi: 10.1172/JCI105238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leaf A. Transepithelial transport and its hormonal control in toad bladder. Ergeb Physiol. 1965;56:216–263. [PubMed] [Google Scholar]
  12. Mendoza S. A., Handler J. S., Orloff J. Effect of amphotericin B on permeability and short-circuit current in toad bladder. Am J Physiol. 1967 Nov;213(5):1263–1268. doi: 10.1152/ajplegacy.1967.213.5.1263. [DOI] [PubMed] [Google Scholar]
  13. Routunno C. A., Pouchan M. I., Cereijido M. Location of the mechanism of active transport of sodium across the frog skin. Nature. 1966 May 7;210(5036):597–599. doi: 10.1038/210597a0. [DOI] [PubMed] [Google Scholar]
  14. Sharp G. W., Leaf A. Mechanism of action of aldosterone. Physiol Rev. 1966 Oct;46(4):593–633. doi: 10.1152/physrev.1966.46.4.593. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES