Abstract
1. The time course and extent of changes in the composition of renal tissue slices in water diuresis were determined by sacrificing groups of rats before and during the intravenous infusion of dextrose (2·5 g/100 ml.) in amounts sufficient to administer over 2 hr, and subsequently to maintain for up to 7½ hr, a positive fluid load of 4% body weight.
2. The corticomedullary osmolal gradient characteristic of the nondiuretic rats was progressively dissipated until, at 7½ hr, only papillary tip concentrations were higher than those of other segments.
3. The changes in individual constituents followed different time courses: (i) an increase in water content in all segments, particularly the papilla, was almost complete by 1 hr, preceding the maximal increases in urine flow; (ii) a marked decrease in papillary and medullary urea content in the first hour was followed by a slower, progressive decrease leading to an almost complete dissipation of the urea gradient by 7½ hr; (iii) small, non-significant decreases in sodium content occurred in all segments in the first hr, followed by a further small, progressive decrease in papillary sodium content; (iv) changes in ammonium and potassium concentrations were mainly related to those in water content, since the contents of these solutes showed only small changes.
4. By 2 hr, differences in the rates of decline of osmolal and urea concentrations in urine and papilla led to urinary concentrations significantly lower than papillary values. The steep papilla-urine urea concentration difference became smaller, but remained significant even at 7½ hr.
5. The findings are discussed in terms of changes in countercurrent mechanisms, particularly as influenced by anti-diuretic hormone.
6. The development of papilla/urine urea concentration ratio greater than unity is also considered in terms of passive transport with changes in membrane permeability.
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Selected References
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- APPELBOOM J. W., BRODSKY W. A., SCOTT W. N. EFFECT OF OSMOTIC DIURESIS ON INTRARENAL SOLUTES IN DIABETES INSIPIDUS AND HYDROPENIA. Am J Physiol. 1965 Jan;208:38–45. doi: 10.1152/ajplegacy.1965.208.1.38. [DOI] [PubMed] [Google Scholar]
- AUKLAND K. Renal tubular permeability to urea with special reference to accumulation of urea in the renal medulla. Scand J Clin Lab Invest. 1961;13:646–660. doi: 10.3109/00365516109137339. [DOI] [PubMed] [Google Scholar]
- Atherton J. C., Hai M. A., Thomas S. Effects of water diuresis and osmotic (mannitol) diuresis on urinary solute excretion by the conscious rat. J Physiol. 1968 Jul;197(2):395–410. doi: 10.1113/jphysiol.1968.sp008566. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atherton J. C., Hai M. A., Thomas S. The time course of changes in renal tissue composition during mannitol diuresis in the rat. J Physiol. 1968 Jul;197(2):411–428. doi: 10.1113/jphysiol.1968.sp008567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERLINER R. W., LEVINSKY N. G., DAVIDSON D. G., EDEN M. Dilution and concentration of the urine and the action of antidiuretic hormone. Am J Med. 1958 May;24(5):730–744. doi: 10.1016/0002-9343(58)90377-2. [DOI] [PubMed] [Google Scholar]
- BOYLAN J. W., ASSHAUER E. Depletion and restoration of the medullary osmotic gradient in the dog kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1962;276:99–116. doi: 10.1007/BF00363327. [DOI] [PubMed] [Google Scholar]
- BRAY G. A., PRESTON A. S. Effect of urea on urine concentration in the rat. J Clin Invest. 1961 Nov;40:1952–1960. doi: 10.1172/JCI104420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berliner R. W., Bennett C. M. Concentration of urine in the mammalian kidney. Am J Med. 1967 May;42(5):777–789. doi: 10.1016/0002-9343(67)90095-2. [DOI] [PubMed] [Google Scholar]
- Clapp J. R. Renal tubular reabsorption of urea in normal and protein-depleted rats. Am J Physiol. 1966 Jun;210(6):1304–1308. doi: 10.1152/ajplegacy.1966.210.6.1304. [DOI] [PubMed] [Google Scholar]
- Foulkes E. C. The action of pitressin on solute permeability of the rabbit nephron in vivo. J Gen Physiol. 1966 Sep;50(1):1–8. doi: 10.1085/jgp.50.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GARDNER K. D., Jr, MAFFLY R. H. AN IN VITRO DEMONSTRATION IN INCREASED COLLECTING TUBULAR PERMEABILITY TO UREA IN THE PRESENCE OF VASOPRESSIN. J Clin Invest. 1964 Oct;43:1968–1975. doi: 10.1172/JCI105070. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOODMAN A., LEVITIN H. SODIUM CONTENT OF THE RENAL MEDULLA DURING OSMOTIC DIURESIS. Yale J Biol Med. 1964 Feb;36:306–310. [PMC free article] [PubMed] [Google Scholar]
- Gardner K. D., Jr Dry weight as a point of reference in studies of renal papillary composition. Am J Physiol. 1966 Oct;211(4):1031–1035. doi: 10.1152/ajplegacy.1966.211.4.1031. [DOI] [PubMed] [Google Scholar]
- Grantham J. J., Burg M. B. Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Am J Physiol. 1966 Jul;211(1):255–259. doi: 10.1152/ajplegacy.1966.211.1.255. [DOI] [PubMed] [Google Scholar]
- JAENIKE J. R. The influence of vasopressin on the permeability of the mammalian collecting duct to urea. J Clin Invest. 1961 Jan;40:144–151. doi: 10.1172/JCI104228. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOBINGER W. DIE WIRKUNG VON VASOPRESSIN AUF DIE KONZENTRATIONSGRADIENTEN VON NATRIUM UND HARNSTOFF IM NIERENGEWEBE BEI VERSCHIEDENEN DIURESEZUSTAENDEN. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 Mar 19;246:538–551. doi: 10.1007/BF00246307. [DOI] [PubMed] [Google Scholar]
- LAMDIN E. Mechanisms of urinary concentration and dilution. AMA Arch Intern Med. 1959 Apr;103(4):644–671. doi: 10.1001/archinte.1959.00270040130015. [DOI] [PubMed] [Google Scholar]
- LEAF A., ANDERSON J., PAGE L. B. Active sodium transport by the isolated toad bladder. J Gen Physiol. 1958 Mar 20;41(4):657–668. doi: 10.1085/jgp.41.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVITIN H., GOODMAN A., PIGEON G., EPSTEIN F. H. Composition of the renal medulla during water diuresis. J Clin Invest. 1962 May;41:1145–1151. doi: 10.1172/JCI104567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LILIEN O. M., JONES S. G., MUELLER C. B. THE MECHANISM OF MANNITOL DIURESIS. Surg Gynecol Obstet. 1963 Aug;117:221–228. [PubMed] [Google Scholar]
- Lassiter W. E., Mylle M., Gottschalk C. W. Micropuncture study of urea transport in rat renal medulla. Am J Physiol. 1966 May;210(5):965–970. doi: 10.1152/ajplegacy.1966.210.5.965. [DOI] [PubMed] [Google Scholar]
- MALVIN R. L., WILDE W. S. Washout of renal countercurrent Na gradient by osmotic diuresis. Am J Physiol. 1959 Jul;197(1):177–180. doi: 10.1152/ajplegacy.1959.197.1.177. [DOI] [PubMed] [Google Scholar]
- MOREL F. F., GUINNEBAULT M., AMIEL C. [Demonstration of a process of water exchange by counter-current in the deep regions of the hamster kidney]. Helv Physiol Pharmacol Acta. 1960;18:183–192. [PubMed] [Google Scholar]
- Martino J. A., Earley L. E. The effects of infusion of water on renal hemodynamics and the tubular reabsorption of sodium. J Clin Invest. 1967 Jul;46(7):1229–1238. doi: 10.1172/JCI105616. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAIKIA T. C. COMPOSITION OF THE RENAL CORTEX AND MEDULLA OF RATS DURING WATER DIURESIS AND ANTIDIURESIS. Q J Exp Physiol Cogn Med Sci. 1965 Apr;50:146–157. doi: 10.1113/expphysiol.1965.sp001777. [DOI] [PubMed] [Google Scholar]
- SAWYER W. H. Neurophypophysial hormones. Pharmacol Rev. 1961 Jun;13:225–277. [PubMed] [Google Scholar]
- SCHMIDT-NIELSEN B. Urea excretion in mammals. Physiol Rev. 1958 Apr;38(2):139–168. doi: 10.1152/physrev.1958.38.2.139. [DOI] [PubMed] [Google Scholar]
- STAHL W. M. EFFECT OF MANNITOL ON THE KIDNEY: CHANGES IN INTRARENAL HEMODYNAMICS. N Engl J Med. 1965 Feb 25;272:382–386. doi: 10.1056/NEJM196502252720801. [DOI] [PubMed] [Google Scholar]
- Scaglione P. R., Dell R. B., Winters R. W. Lactate concentration in the medulla of rat kidney. Am J Physiol. 1965 Dec;209(6):1193–1198. doi: 10.1152/ajplegacy.1965.209.6.1193. [DOI] [PubMed] [Google Scholar]
- THOMAS S. SOLUTE EXCRETION IN MAN DURING CHANGING URINE FLOW OCCURRING SPONTANEOUSLY AND INDUCED BY VASOPRESSIN INJECTION. J Clin Invest. 1964 Jan;43:1–10. doi: 10.1172/JCI104883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THURAU K., DEETJEN P., KRAMER K. [Hemodynamics of kidney medullary substance. Part II. Interrelationships between the vascular and tubular counter-flow system in arterial pressure increases, water diuresis and osmotic diuresis]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:270–285. [PubMed] [Google Scholar]
- THURAU K. RENAL HEMODYNAMICS. Am J Med. 1964 May;36:698–719. doi: 10.1016/0002-9343(64)90181-0. [DOI] [PubMed] [Google Scholar]
- TRUNIGER B., SCHMIDT-NIELSEN B. INTRARENAL DISTRIBUTION OF UREA AND RELATED COMPOUNDS: EFFECTS OF NITROGEN INTAKE. Am J Physiol. 1964 Nov;207:971–978. doi: 10.1152/ajplegacy.1964.207.5.971. [DOI] [PubMed] [Google Scholar]
- ULLRICH K. J., DRENCKHAHN F. O., JARAUSCH K. H. Untersuchungen zum Problem der Harnkonzentrierung und -verdünnung; uber das osmotische Verhalten von Nierenzellen und die begieitende Elektrolytanhäufung im Nierengewebe bei verschiedenen Diuresezuständen. Pflugers Arch. 1955;261(1):62–77. doi: 10.1007/BF00363541. [DOI] [PubMed] [Google Scholar]
- ULLRICH K. J., KRAMER K., BOYLAN J. W. Present knowledge of the counter-current system in the mammalian kidney. Prog Cardiovasc Dis. 1961 Mar;3:395–431. doi: 10.1016/s0033-0620(61)80001-7. [DOI] [PubMed] [Google Scholar]
- USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]
- Valtin H. Sequestration of urea and nonurea solutes in renal tissues of rats with hereditary hypothalamic diabetes insipidus: effect of vasopressin and dehydration on the countercurrent mechanism. J Clin Invest. 1966 Mar;45(3):337–345. doi: 10.1172/JCI105348. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zain-ul-Abedin Effects of vasopressin upon the composition of rat's kidney. Q J Exp Physiol Cogn Med Sci. 1967 Jul;52(3):285–292. doi: 10.1113/expphysiol.1967.sp001914. [DOI] [PubMed] [Google Scholar]