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. 1977 Aug;270(1):195–208. doi: 10.1113/jphysiol.1977.sp011946

The effects of external sodium substitution on cell sodium and potassium in vascular smooth muscle

Sydney M Friedman
PMCID: PMC1353425  PMID: 915771

Abstract

1. The entry of Li into the vascular smooth muscle cells of the rat tail artery follows first-order kinetics with a rate constant of approximately 1·3 hr-1 at 10 mM-[Li]o. The rate constant decreases gradually to ca. 0·5 hr-1 when the [Li]o/[Na]o ratio is increased.

2. Replacement of Na with Li over the range of [Li]o from 1 to 115 mM, accomplished at constant ionic strength and osmolarity of the bathing solution, produces changes in cell Na and K without apparent change in cell water. At equilibrium, cell Li increases in linear proportion to [Li]o, at a ratio of 2:1 throughout the range. The increase in cell Li is associated with inverse falls in both cell K and Na such that the ratio of cell K to cell Na remains constant at ca. 10:1 throughout.

3. The changes in the ionic contents, induced by equilibration of the tissue with a Na-free, Li-substituted solution, are reversible.

4. Replacement of Na with sucrose over the range of 40-115 mM results, at equilibrium, in a linear fall in cell Na without conspicuous change in cell K. A constant portion of the cell Na, ca. 10 m-mole/kg dry wt., does not participate in this exchange.

5. At equilibrium, reductions in [Na]o are reflected in corresponding reductions in apparent [Na]i such that the [Na]o/[Na]i ratio remains constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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