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. 1977 Dec;273(3):631–645. doi: 10.1113/jphysiol.1977.sp012114

The effect of 2,3-diphosphoglycerate on the oxygen dissociation curve of human haemoglobin.

P J Goodford, F E Norrington, R A Paterson, R Wootton
PMCID: PMC1353752  PMID: 604451

Abstract

1. Oxygen dissociation curves for concentrated human haemoglobin solutions (1.6 mmol dm-3 in haem) have been measured by mixing known quantities of oxy- and deoxyhaemoglobin solutions and measuring the resulting partial pressure of oxygen with an oxygen electrode. 2. Observations in the presence of 2,3-diphosphoglycerate support previous conclusions derived from experiments at low haemoglobin concentrations, the validity of which has been questioned. 3. The two affinity state model of Monod, Wyman & Changeux (1965) does not fully describe the actions of 2,3-diphosphoglycerate and a model in which this allosteric effector not only binds preferentially to the T state but also lowers the oxygen affinity of this state gives an improved fit to the data.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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