Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1977 Dec;273(3):745–764. doi: 10.1113/jphysiol.1977.sp012121

Studies on the mechanism of renin release from isolated superfused rat glomeruli: effects of calcium, calcium ionophore and lanthanum

L Baumbach, P P Leyssac
PMCID: PMC1353759  PMID: 415132

Abstract

1. The effects of external medium calcium concentration, the ionophore A23187 and lanthanum on the rate of renin release in vitro were studied with particular emphasis on results obtained from isolated superfused glomeruli of rat kidneys.

2. The response to reduction in superfusate calcium concentration from 2 mM was a graded and reversible increase in the rate of renin release. An increase in release was detectable at 0.2 mM calcium; a threefold increase was found 36 min after a change from 2 mM calcium to calcium-free superfusate. A similar relative increase in release resulted from reductions from 0.1 mM to zero calcium, but the absolute amounts of renin released were greater in this latter series. Renin release from kidney cortical slices similarly increased in response to calcium-free incubation medium.

3. The effects of A23187 on renin release were modest. Changing from 2 mM calcium during control periods to calcium-free Ringer with A23187 added caused an attenuated and more delayed increase in release than the change to calcium-free Ringer without ionophore. This difference in response was abolished when glomeruli were superfused with 0.1 mM calcium during the preceding 1 hr control period. There was no significant difference in renin release from glomeruli exposed to calcium-free EGTA-Ringer with and without A23187 in the 2 mM calcium series; in the 0.1 mM calcium series the increase in release following a shift to calcium-free EGTA-containing superfusate with A23187 added was significantly greater than in the absence of the ionophore.

4. Addition of lanthanum (1 or 0.05 mM) to calcium-containing as well as calcium-free superfusate resulted in a significant depression of renin release. Subsequent removal of the lanthanum did not restore the rate of release unless EGTA was added; in the latter case a massive increase in renin release occurred resulting in a marked depletion of the remaining renin content of the glomeruli.

5. It is concluded that calcium influences renin release by a direct action on the juxtaglomerular cells. The data support the previous suggestion that basal renin release is a function of active, calcium-dependent cell volume regulation — swelling causing an increase in the release; and further suggest that membrane-bound calcium has a direct effect on the cell membrane permeability to renin.

6. The results exclude that calcium-stimulated exocytosis is responsible for basal renin release from the juxtaglomerular cells adhering to isolated glomeruli.

Full text

PDF
745

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoi W., Wade M. B., Rosner D. R., Weinberger M. H. Renin release by rat kidney slices in vitro: effects of cations and catecholamines. Am J Physiol. 1974 Sep;227(3):630–634. doi: 10.1152/ajplegacy.1974.227.3.630. [DOI] [PubMed] [Google Scholar]
  2. Baumbach L., Leyssac P. P., Skinner S. L. Studies on renin release from isolated superfused glomeruli: effects of temperature, urea, ouabain and ethacrynic acid. J Physiol. 1976 Jun;258(1):243–256. doi: 10.1113/jphysiol.1976.sp011417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blendstrup K., Leyssac P. P., Poulsen K., Skinner S. L. Characteristics of renin release from isolated superfused glomeruli in vitro. J Physiol. 1975 Apr;246(3):653–672. doi: 10.1113/jphysiol.1975.sp010909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COOK W. F., PICKERING G. W. The location of renin in the rabbit kidney. J Physiol. 1959 Dec;149:526–536. doi: 10.1113/jphysiol.1959.sp006359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corsini W. A., Crosslan K. L., Bailie M. D. Renin secretion by rat kidney slices in vitro. Proc Soc Exp Biol Med. 1974 Feb;145(2):403–406. doi: 10.3181/00379727-145-37819. [DOI] [PubMed] [Google Scholar]
  6. DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis J. O., Freeman R. H. Mechanisms regulating renin release. Physiol Rev. 1976 Jan;56(1):1–56. doi: 10.1152/physrev.1976.56.1.1. [DOI] [PubMed] [Google Scholar]
  8. Douglas W. W., Poisner A. M. The influence of calcium on the secretory response of the submaxillary gland to acetylcholine or to noradrenaline. J Physiol. 1963 Mar;165(3):528–541. doi: 10.1113/jphysiol.1963.sp007076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Frederiksen O., Leyssac P. P., Skinner S. L. Sensitive osmometer function of juxtaglomerular cells in vitro. J Physiol. 1975 Nov;252(3):669–679. doi: 10.1113/jphysiol.1975.sp011164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hong J. S., Poisner A. M. Properties of renin granules isolated from rat kidney. Mol Cell Endocrinol. 1976 Oct;5(5):331–337. doi: 10.1016/0303-7207(76)90015-0. [DOI] [PubMed] [Google Scholar]
  11. Kotchen T. A., Mauli K. I., Luke R., Rees D., Flamenbaum W. Effect of acute and chronic calcium administration on plasma renin. J Clin Invest. 1974 Dec;54(6):1279–1286. doi: 10.1172/JCI107873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Michelakis A. M. The effect of sodium and calcium on renin release in vitro. Proc Soc Exp Biol Med. 1971 Jul;137(3):833–836. doi: 10.3181/00379727-137-35677. [DOI] [PubMed] [Google Scholar]
  13. Morris B. J., Nixon R. L., Johnston C. I. Release of renin from glomeruli isolated from rat kidney. Clin Exp Pharmacol Physiol. 1976 Jan-Feb;3(1):37–47. doi: 10.1111/j.1440-1681.1976.tb00589.x. [DOI] [PubMed] [Google Scholar]
  14. Rangachari P. K., Daniel E. E., Paton D. M. Regulation of cellular volume in rat myometrium. Biochim Biophys Acta. 1973 Oct 11;323(2):297–308. doi: 10.1016/0005-2736(73)90153-3. [DOI] [PubMed] [Google Scholar]
  15. Rorive G., Kleinzeller A. The effect of ATP and Ca 2+ on the cell volume in isolated kidney tubules. Biochim Biophys Acta. 1972 Jul 3;274(1):226–239. doi: 10.1016/0005-2736(72)90296-9. [DOI] [PubMed] [Google Scholar]
  16. Rorive G., Nielsen R., Kleinzeller A. Effect of pH on the water and electrolyte content of renal cells. Biochim Biophys Acta. 1972 May 9;266(2):376–396. doi: 10.1016/0005-2736(72)90095-8. [DOI] [PubMed] [Google Scholar]
  17. Saruta T., Matsuki S. The effects of cyclic AMP, theophylline, angiotensin II and electrolytes upon renin release from rat kidney slices. Endocrinol Jpn. 1975 Apr;22(2):137–140. doi: 10.1507/endocrj1954.22.137. [DOI] [PubMed] [Google Scholar]
  18. Van Dongen R., Peart W. S. Calcium dependence of the inhibitory effect of angiotensin on renin secretion in the isolated perfused kidney of the rat. Br J Pharmacol. 1974 Jan;50(1):125–129. doi: 10.1111/j.1476-5381.1974.tb09599.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vandongen R., Peart W. S. The inhibition of renin secretion by alpha-adrenergic stimulation in the isolated rat kidney. Clin Sci Mol Med. 1974 Nov;47(5):471–479. doi: 10.1042/cs0470471. [DOI] [PubMed] [Google Scholar]
  20. Viskoper R. J., Rosenfeld S., Maxwell M. H., De Lima J., Lupu A. N., Rosenfeld J. B. Effect of Ca2+ binding by EGTA on renin release in the isolated perfused rabbit kidney. Proc Soc Exp Biol Med. 1976 Jul;152(3):415–418. doi: 10.3181/00379727-152-39409. [DOI] [PubMed] [Google Scholar]
  21. Watkins B. E., Davis J. O., Lohmeier T. E., Freeman R. H. Intrarenal site of action of calcium on renin secretion in dogs. Circ Res. 1976 Dec;39(6):847–853. doi: 10.1161/01.res.39.6.847. [DOI] [PubMed] [Google Scholar]
  22. Yamamoto K., Iwao H., Abe Y., Morimoto S. Effect of Ca on renin release in vitro and in vivo. Jpn Circ J. 1974 Dec;38(12):1127–1131. doi: 10.1253/jcj.38.1127. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES