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. 2026 Sep 8;16:28078. doi: 10.1038/s41598-026-70774-1

Correction: Development of a novel liquid chromatography-tandem mass spectrometry based enzymatic assay of 5,10-methylenetetrahydrofolate reductase

Kohei Sunoki 1, Miyuki Watanabe 1, Shiho Aoki 1, Eriko Jimbo 1, Hiroko Shimbo 2, Tomohide Goto 2, Hitoshi Osaka 1,✉
PMCID: PMC13554228  PMID: 42711370

Correction to: Scientific Reports https://doi.org/10.1038/s41598-025-07926-2, published online 02 July 2025

The original version of this Article contained an error in the FAD concentration. Consequently, in the Materials and Methods section, under the subheading ‘Enzyme assays’,

“The standard assay mixture consisted of 0.05 mol/L potassium phosphate buffer (pH 6.6), 100 µmol/L 5,10-methylenetetrahydrofolate (Schircks Laboratories, Bauma, Switzerland), 200 µmol/L NADPH (Merck KGaA, Darmstadt, Germany), 5 µmol/L FAD (Merck KGaA), and enzyme extract containing 10–70 µg of protein, for a final volume of 100 µL.”

now reads:

“The standard assay mixture consisted of 0.05 mol/L potassium phosphate buffer (pH 6.6), 100 µmol/L 5,10-methylenetetrahydrofolate (Schircks Laboratories, Bauma, Switzerland), 200 µmol/L NADPH (Merck KGaA, Darmstadt, Germany), 75 µmol/L FAD (Merck KGaA), and enzyme extract containing 10–70 µg of protein, for a final volume of 100 µL.”

The original Article has been corrected.


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