Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 2002 Sep;22(18):6533–6541. doi: 10.1128/MCB.22.18.6533-6541.2002

The SMN Complex Is Associated with snRNPs throughout Their Cytoplasmic Assembly Pathway

Séverine Massenet 1, Livio Pellizzoni 1, Sergey Paushkin 1, Iain W Mattaj 2, Gideon Dreyfuss 1,*
PMCID: PMC135628  PMID: 12192051

Abstract

The common neurodegenerative disease spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN associates with several proteins (Gemin2 to Gemin6) to form a large complex which is found both in the cytoplasm and in the nucleus. The SMN complex functions in the assembly and metabolism of several RNPs, including spliceosomal snRNPs. The snRNP core assembly takes place in the cytoplasm from Sm proteins and newly exported snRNAs. Here, we identify three distinct cytoplasmic SMN complexes, each representing a defined intermediate in the snRNP biogenesis pathway. We show that the SMN complex associates with newly exported snRNAs containing the nonphosphorylated form of the snRNA export factor PHAX. The second SMN complex identified contains assembled Sm cores and m3G-capped snRNAs. Finally, the SMN complex is associated with a preimport complex containing m3G-capped snRNP cores bound to the snRNP nuclear import mediator snurportin1. Thus, the SMN complex is associated with snRNPs during the entire process of their biogenesis in the cytoplasm and may have multiple functions throughout this process.

The small nuclear ribonucleoprotein particles (snRNPs) consist of snRNAs (U1, U2, U4/U6, or U5), several specific proteins that are unique to each snRNA, and a set of seven common Sm proteins (B/B′, D1, D2, D3, E, F, and G). The biogenesis of the snRNPs is a complex process that involves both the nucleus and the cytoplasm (for a recent review, see reference 59). The snRNAs, with the exception of U6, are transcribed by RNA polymerase II as precursors that contain additional nucleotides at the 3′ end and a monomethylated m7GpppG (m7G) cap structure at the 5′ end. This cap structure is recognized by the nuclear cap binding complex (CBC), a heterodimeric complex composed of two subunits, CBP20 and CBP80, both of which are required for binding to the m7G cap structure (30, 31, 35, 36). The adaptor protein PHAX binds both CBC and snRNAs and mediates their interaction with the nuclear export receptor CRM1/Exportin1 (Xpo1) (49, 57). CRM1, together with RanGTP, exports the newly transcribed snRNAs to the cytoplasm (9, 13, 29). In vitro, phosphorylation of PHAX is required for the formation of the snRNA export complex but is not necessary for the formation of the precomplex containing snRNAs, CBC, and PHAX but not RanGTP and CRM1 (49). Following export to the cytoplasm, GTP hydrolysis of Ran and dephosphorylation of PHAX lead to disassembly of the snRNA export complex (49). Each snRNA then associates with the Sm proteins, which form a seven-membered ring (snRNP core particle) around the Sm site (34, 59). A properly assembled Sm core is required for cap hypermethylation and 3′-end maturation (40, 48, 56). Both a properly assembled Sm core and an m3G cap structure are required for snRNP import into the nucleus (11, 12, 24, 25, 33, 41). The m3G cap structure is specifically bound by snurportin1, which then interacts with the nuclear import receptor importin-β and, together with an unidentified import receptor that recognizes the Sm core, mediates the import of the assembled snRNP (12, 28, 50).

The neuromuscular disease spinal muscular atrophy (SMA) is characterized by degeneration of motor neurons of the spinal cord leading to muscular weakness and atrophy (reviewed in reference 45). Over 98% of SMA patients have mutations or deletions of the survival of motor neurons 1 (SMN1) gene, and decreased levels of the SMN protein correlate with the phenotypic severity of SMA (7, 19, 27, 32, 45, 46). The SMN protein is expressed in all tissues of metazoan organisms. SMN is associated with several proteins, including Gemin2 (formerly SIP1) (39), the DEAD box RNA helicase Gemin3 (3, 4), Gemin4 (5, 42), a WD repeat protein, Gemin5 (23, 43), and Gemin6 (52) to form large complexes. The SMN complex is found both in the nucleus and in the cytoplasm and appears to be involved in the assembly, restructuring and metabolism of several RNPs, including snRNPs, snoRNPs, and transcriptosomes (2, 10, 39, 53, 55).

Previous experiments have shown that the SMN complex functions in the cytoplasmic assembly of snRNP core particles. Microinjections of anti-SMN complex antibodies in Xenopus oocytes inhibit or stimulate snRNP core particle formation (2, 10), and expression of a dominant-negative mutant of SMN in mammalian cells sequesters Sm proteins and snRNAs in cytoplasmic accumulations (55). Moreover, the SMN complex is required for the assembly of U1 snRNP cores in Xenopus laevis egg extracts (43). SMN binds preferentially and directly to the symmetrical dimethylarginine-modified RG-rich domains of SmD1, SmD3, and SmB (15, 16). This modification is carried out by the methylosome, a complex containing the methyltransferase JBP1 (PRMT5), and it likely serves to direct the Sm proteins to the SMN complex (17, 18, 44). Several SMN mutants found in SMA patients are defective in Sm protein binding, suggesting that a defect in these interactions may play a role in the pathogenesis of SMA (2, 53).

To determine more precisely the role of the SMN complex in snRNP core assembly, we asked at what step the SMN complex interacts with snRNAs and whether the SMN complex is released from the snRNP after Sm core assembly. We show that the SMN complex binds newly exported snRNAs in an RNA-dependent manner and remains associated with the snRNPs during Sm core assembly, m3G cap formation, and snurportin1 binding to the m3G cap structure. These findings indicate that the SMN complex is directly associated with snRNPs during the various steps of their biogenesis in the cytoplasm.

MATERIALS AND METHODS

DNA constructs and antibodies.

Plasmids expressing myc-tagged SMN and SMNΔN27 were described previously (55). The DNA fragment corresponding to the open reading frame of snurportin1 was generated by PCR amplification using specific primers. For transient expression in HeLa cells, the insert was cloned downstream of the cytomegalovirus promoter into a modified pcDNA3 vector (InVitrogen) containing the Flag tag sequence (17).

The antibodies used in these experiments were as follows: anti-SMN (2B1) (38), anti-Gemin2 (2E17) (39), anti-Gemin3 (11G9) (4), anti-Gemin4 (22C10) (5), anti-Gemin5 (10G11) (23), anti-Gemin6 (6H5) (60), anti-importin-β (31H4) (A. Perkinson and G. Dreyfuss, unpublished data), anti-Sm proteins (Y12) (37), anti-2,2,7 trimethylguanosine (ab1; Oncogene Research) (R1131) (11), anti-myc (9E10), anti-poly(A)-binding protein (10E10) (21), anti-Flag (Sigma), anti-PHAX (49), anti-Ran (Perkinson and Dreyfuss, unpublished), anti-CRM1 (Transduction Laboratories), and nonimmune antibody SP2/0 (6).

Cell culture and transfection.

293 cells or HeLa cells were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum. Cells growing on 100-mm-diameter culture dishes (about 40% confluent) were transfected with 5 μg of DNA using the CalPhos Mammalian Transfection kit (Clontech Laboratories) according to the manufacturer's recommendations. For immunofluorescence staining, HeLa cells plated on glass coverslips were transfected with 4 μg of myc-SMNΔN27-expressing vector. Following overnight incubation with DNA, the cells were washed, and fresh medium was added. The transfected cells were fixed and processed by immunofluorescence staining after 48 h of incubation.

Immunofluorescence microscopy.

Immunofluorescence staining was carried out as previously described (55). Laser confocal fluorescence microscopy was performed with a Leica (Bensheim, Germany) TCS 4D confocal microscope. Images from each channel were recorded separately and then merged.

Immunoprecipitation experiments.

Cytoplasmic extracts were prepared as previously described (58) and incubated with specific antibodies bound to GammaBind G Sepharose (Amersham) for 2 h at 4°C in RSB100 (10 mM Tris-HCl [pH 7.5], 100 mM NaCl, 2.5 mM MgCl2) containing 0.01% NP-40. The beads were extensively washed with RSB200 (10 mM Tris-HCl [pH 7.5], 200 mM NaCl, 2.5 mM MgCl2) containing 0.05% NP-40, and the immunoprecipitated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting as previously described (39). For RNA analysis, bound RNAs were isolated as previously described (10), 3′-end labeled with [5′-32P]pCp (3,000 Ci/mmol; Amersham) and T4 RNA ligase according to the method of England and Uhlenbeck (8), and separated on a denaturing 10% polyacrylamide gel containing 7 M urea. The bands were visualized by autoradiography.

RESULTS

The SMN complex is associated with newly exported PHAX-containing snRNAs.

To investigate a possible interaction between the SMN complex and the snRNA export complex in vivo, HeLa cell cytoplasmic extracts were prepared and immunoprecipitations were carried out using either anti-SMN (2B1), anti-PHAX, or nonimmune (control) antibody (Fig. 1). The quality of the cellular fractionation was monitored by the presence in cytoplasmic and nuclear fractions of the nuclear-restricted hnRNP C protein (compare Fig. 1B and C). The immunoprecipitated proteins were then analyzed by SDS-PAGE and Western blotting. The snRNA export factor PHAX is the earliest detectable marker of newly exported snRNAs in the cytoplasm (49). Phosphorylated PHAX is associated with the snRNAs in the nucleus, and following snRNA export, PHAX is dephosphorylated (49). The anti-SMN antibody specifically coimmunoprecipitated the nonphosphorylated form of PHAX from the cytoplasm (Fig. 1A). In contrast, the poly(A)-binding protein (PABP), as a control, was not coimmunoprecipitated with the anti-SMN antibody. The interaction between the SMN complex and PHAX in the cytoplasm is RNA dependent, because PHAX was not coimmunoprecipitated with SMN after RNase treatment. No dephosphorylation of PHAX occurred in the extract during the course of the immunoprecipitation experiment (data not shown). In a reciprocal experiment (Fig. 1B and C), all the known components of the SMN complex, namely, SMN, Gemin2, Gemin3, Gemin4, Gemin5, and Gemin6, were coimmunoprecipitated from the cytoplasm in an RNA-dependent manner using the anti-PHAX antibody. No interaction between PHAX and SMN was detected in the nuclear fraction (Fig. 1C). These findings indicate that the SMN complex is associated with snRNAs (10, 60) and nonphosphorylated PHAX in the cytoplasm but not in the nucleus. Even though PHAX can bind directly to snRNAs in vitro, PHAX interaction with the snRNA export complex requires its binding to both CBC and snRNAs (57). Therefore, snRNAs and CBC bound to the m7G cap structure likely mediate the association between nonphosphorylated PHAX and the SMN complex. Unfortunately, we were not able to probe for the components of the CBC complex due to the limited affinities of available anti-CBC antibodies. SmB also coimmunoprecipitated with PHAX in an RNA-dependent manner (Fig. 1B). Finally, anti-PHAX antibody did not coimmunoprecipitate Ran and CRM1, likely because RanGTP hydrolysis occurs immediately after snRNA export (Fig. 1B). Therefore, the SMN complex associates with Sm proteins and the postexport complex that contains nonphosphorylated PHAX, CBC, and snRNAs.

FIG. 1.

FIG. 1.

Open in a new tab

Association of the SMN complex with PHAX in vivo. Immunoprecipitation (IP) experiments were carried out on HeLa cell cytoplasmic extracts (Cytoplasm) or on HeLa cell nucleoplasmic extracts (Nucleoplasm) using anti-SMN (2B1) or nonimmune (Control) antibody (A) and anti-PHAX (α-PHAX) or nonimmune (Control) antibody (B and C) in the presence (+) or absence (−) of RNase. The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting with antibodies to the indicated proteins; 2% of the inputs are shown (Total). P-PHAX, the phosphorylated form of PHAX. PABP, poly(A)-binding protein.

The SMN complex is associated with m3G cap-containing snRNPs in the cytoplasm.

Subsequent to snRNP core assembly, the m7G cap structure of the snRNAs is converted to an m3G cap structure, and the snRNAs undergo 3′-end trimming. Both events are dependent on proper snRNP core assembly (see the introduction). The snRNP core assembly likely takes place in association with the SMN complex. To determine if the SMN complex is still associated with the snRNPs after Sm core assembly, HeLa cell cytoplasmic extracts were prepared and immunoprecipitated with anti-m3G cap (TMG) or nonimmune (SP2/0) antibody (Fig. 2). SnRNP cores were efficiently coimmunoprecipitated with anti-m3G cap antibody, as indicated by the presence of SmB and snRNAs (Fig. 2 and data not shown). All the components of the SMN complex were also coimmunoprecipitated with the m3G-capped snRNP core. In contrast, PHAX, as well as PABP as a control, were not coimmunoprecipitated with the anti-m3G cap antibody. Thus, the SMN complex does not dissociate following Sm core assembly and remains bound to snRNPs after cap hypermethylation whereas PHAX is removed before hypermethylation. The dissociation of PHAX and CBC might be expected to expose the m7G cap structure, allowing the cap methyltransferase access to it. The association between the SMN complex and cytoplasmic m3G-capped snRNPs strongly suggests that snRNP core assembly, as well as the formation of the m3G cap structure, occur in the presence of the SMN complex.

FIG. 2.

FIG. 2.

Open in a new tab

Association of the SMN complex with m3G-capped snRNAs in the cytoplasm. Immunoprecipitation (IP) experiments were carried out on HeLa cell cytoplasmic extracts using anti-m3G cap structure (TMG) or nonimmune (SP2/0) antibody. The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting with antibodies to the indicated proteins; 2% of the inputs are shown (Total). P-PHAX, the phosphorylated form of PHAX. PABP, poly(A)-binding protein.

The SMN complex is associated with snurportin1-containing preimport snRNPs.

The next step in the pathway of snRNP biogenesis is the binding of snurportin1 to the newly formed m3G cap structure, which in turn recruits importin-β and, together with an unidentified import receptor that recognizes the Sm core, mediates snRNP import into the nucleus (28, 50). To test whether there is an interaction between the SMN complex and snRNPs bound to snurportin1, cytoplasmic extracts were prepared from 293T cells transiently expressing Flag-tagged snurportin1 (Flag-snurportin1) or the Flag tag alone (Mock) and immunoprecipitated with anti-Flag antibodies. The immunoprecipitated proteins were then analyzed by Western blotting (Fig. 3A), and the immunoprecipitated RNAs were analyzed by denaturing polyacrylamide electrophoresis after 3′-end labeling (Fig. 3B). SmB and the snRNAs (U1, U2, U4, and U5) were specifically coimmunoprecipitated with snurportin1. We note that snurportin1 also associates with U11 snRNA, suggesting that the minor and major snRNAs likely share the same import pathway. snurportin1 was associated with importin-β, as well as with the SMN complex. When the cytoplasmic extracts were treated with RNase during immunoprecipitations, SmB and the SMN complex components were not coimmunoprecipitated with Flag-snurportin1, indicating that snurportin1 and the SMN complex are associated with the same RNPs but do not bind each other. As expected, the interaction between snurportin1 and importin-β was stable under these conditions. Thus, the SMN complex is associated with the last preimport snRNP intermediate, which contains snurportin1.

FIG. 3.

FIG. 3.

Open in a new tab

Association of the SMN complex with snurportin1 in vivo. Immunoprecipitation (IP) experiments were carried out on cytoplasmic extracts prepared from 293 cells transiently expressing Flag-snurportin1 (Flag-snurportin1) or Flag-pcDNA3 alone (Mock). (A) Immunoprecipitation experiments were carried out using anti-Flag antibody in the presence (+) or absence (−) of RNase. The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting with antibodies to the indicated proteins; 2% of the inputs are shown (Total). P-PHAX, the phosphorylated form of PHAX. (B) Immnunoprecipitation experiments were carried out using anti-Sm proteins (Y12), anti-m3G cap structure (TMG), anti-Flag (Flag), and nonimmune (SP2/0) antibodies. Immunoprecipitated RNAs were extracted from the beads, 3′-end labeled, analyzed by denaturing polyacrylamide gel electrophoresis, and visualized by autoradiography. The immunoprecipitated RNAs are indicated by arrows (20).

Expression of the SMN dominant-negative mutant, SMNΔN27, causes reorganization of PHAX in the cytoplasm.

PHAX localizes in the nucleoplasm and is highly concentrated in nuclear domains (57). Frey and Matera (14) showed that green fluorescent protein-PHAX accumulates in the Cajal (coiled) bodies. We analyzed the localization of PHAX in HeLa cells (strain PV), where gems and Cajal bodies are often observed as separate structures (38). As shown by costaining with anti-coilin and anti-SMN antibodies, endogenous PHAX accumulates in Cajal bodies (Fig. 4A to C) but not in gems (Fig. 4D to F). The presence of PHAX in the Cajal bodies is consistent with the possibility that these nuclear bodies are the site of early events of snRNP biogenesis (14).

FIG. 4.

FIG. 4.

Open in a new tab

In the nucleus, endogenous PHAX accumulates in Cajal bodies. A double-label confocal immunofluorescence experiment using anti-coilin (A), anti-PHAX (B and E), and anti-SMN (D) antibodies on HeLa cells is shown. The respective combined images are shown in panels C and F. Colocalization results in a yellow signal. The dashed lines demarcate the nuclei. The Cajal bodies (C) and the gems (F) are indicated by arrows.

The SMN dominant-negative mutant, SMNΔN27, inhibits splicing in vitro (55). In vivo, the expression of SMNΔN27 causes a profound rearrangement of snRNPs, snoRNPs, and components of the RNA polymerase II transcription and processing machinery and inhibits transcription in the nucleus (51, 54, 55). SMNΔN27 also leads to the cytoplasmic accumulation of endogenous SMN complexes, Sm proteins, and m7G-capped snRNAs in discrete accumulations, indicating that this mutant blocks the snRNP assembly pathway prior to m3G cap formation (55). We investigated whether the expression of SMNΔN27 has an effect on the subcellular localization of PHAX in HeLa cells. As shown in Fig. 5A to F, PHAX specifically accumulates with both SMNΔN27 and Sm proteins in the cytoplasmic but not in the nuclear accumulations. In cells transfected with wild-type SMN, no reorganization of PHAX or Sm proteins was observed (reference 55 and data not shown). The accumulation of PHAX in these cytoplasmic accumulations is consistent with the association between the SMN complex and the newly exported PHAX-containing snRNAs detected by coimmunoprecipitation experiments (Fig. 1). In cells transfected with SMNΔN27, gems and Cajal bodies are completely merged in large nuclear accumulations (55). Consistent with the presence of PHAX in Cajal bodies (Fig. 4B), low levels of PHAX colocalize with SMNΔN27 in the nuclei of SMNΔN27-tranfected cells. However, nucleoplasmic PHAX does not accumulate in the enlarged Cajal bodies or gem accumulations.

FIG.5.

FIG.5.

Open in a new tab

Expression of SMNΔN27 causes accumulation of PHAX, but not snurportin1 and importin-β in the cytoplasm. (A and B) Double-label confocal immunofluorescence experiment using anti-myc (A) and anti-PHAX (B) antibodies on HeLa cells transiently expressing myc-SMNΔN27. (D and E) Double-label confocal immunofluorescence experiment using anti-Sm protein (D) and anti-PHAX (E) antibodies on HeLa cells transiently expressing myc-SMNΔN27. (G and H) Double-label confocal immunofluorescence experiment using anti-myc (G) and anti-Flag (H) antibodies on HeLa cells transiently expressing myc-SMNΔN27 and Flag-snurportin1. (J and K) Double-label confocal immunofluorescence experiment using anti-myc (J) and anti-importin-β (K) antibodies on HeLa cells transiently expressing myc-SMNΔN27. The respective combined images are shown in panels C, F, I, and L. Colocalization results in a yellow signal. The dashed lines demarcate the nuclei. The cytoplasmic accumulations are indicated by arrows. The different sizes of the nuclear and cytoplasmic accumulations observed in panels A to C and panels D to F are due to different incubation times of the cells after transfection.

Flag-snurportin1 mostly localizes in the cytoplasm (data not shown). In cells cotransfected with myc-SMNΔN27 and Flag-snurportin1, Flag-snurportin1 does not accumulate with the mutant SMNΔN27 in either the cytoplasmic or the nuclear accumulations (Fig. 5G to I) and shows the same localization observed in cells transfected with wild-type SMN or transfected only with Flag-snurportin1 (data not shown). Similarly, importin-β does not accumulate in the cytoplasmic accumulations (Fig. 5J to L). We note that some importin-β localizes within the SMNΔN27 nuclear accumulations. The significance of this observation is not known. The absence of snurportin1 and importin-β in the cytoplasmic accumulations is consistent with the suggestion that SMNΔN27 blocks the snRNP assembly pathway prior to cap hypermethylation.

DISCUSSION

Over the past several years, it has become evident that the SMN complex functions in several aspects of mRNA biogenesis, such as splicing and the assembly of various RNPs in cells. Our experiments reveal not only that the SMN complex plays a role in proper Sm core assembly but also that it is associated with the snRNPs throughout their biogenesis in the cytoplasm. We found that the SMN complex is associated with three distinct snRNP complexes in the cytoplasm, each representing a well-defined intermediate in the pathway of snRNP assembly that spans the entire cytoplasmic phase. Figure 6 presents a model depicting these different complexes and summarizing the current view of the role of the SMN complex in this pathway.

FIG. 6.

FIG. 6.

Open in a new tab

Schematic model of the role of SMN in snRNP core biogenesis in the cytoplasm. The cytoplasmic chaperone function of the SMN complex in snRNP core formation is discussed in the text. The methylosome consists of the MEP50, pICln, and JBP1 (PRMT5) proteins (16, 17, 44). sDMA, symmetrical dimethylarginine; CB, cajal body.

To accomplish its function in snRNP core assembly, the SMN complex must bring together the Sm proteins and the snRNAs. The SMN complex contains all seven Sm proteins, and SMN binds directly to a subset of Sm proteins including SmB, SmD1, and SmD3 (2, 4, 5, 15, 39, 53). Methylation of specific arginine residues in the RG-rich domains of SmB, SmD1, and SmD3 by the methylosome dramatically increases their affinity for SMN and thus directs them to the SMN complex (Fig. 6) (1, 16-18, 44). The SMN complex also has the capacity to directly interact with the U1 snRNA (60). We detected an RNA-dependent association between the nonphosphorylated form of PHAX and the Sm-containing SMN complex in the cytoplasm but not in the nucleus. This indicates that the SMN complex binds the newly exported snRNAs (Fig. 6), and the direct interaction of SMN complexes with U1 snRNA likely facilitates this association (60). The interaction between PHAX and the Sm-containing SMN complex occurs after hydrolysis of RanGTP and dephosphorylation of PHAX. These two events are believed to lead to the dissociation of Ran and CRM1 from the snRNAs and therefore to the disassembly of the snRNA export complex immediately after snRNA export into the cytoplasm (49). Both in vivo and in vitro, nonphosphorylated PHAX, CBC, and snRNAs form a stable complex (this work and reference 49). It is not yet known how PHAX and CBC are removed from the snRNAs, but the interaction between CBC and the importin-α/β heterodimer can release capped RNAs from CBC (22). Our results do not address whether the PHAX/CBC complex is released before or after Sm core assembly. The dominant-negative SMNΔN27 mutant causes accumulation of the SMN complex, Sm proteins, and m7G cap snRNAs in the cytoplasm (55). This suggested that SMNΔN27 blocks the snRNP pathway in the cytoplasm at a step preceding cap hypermethylation (55). The observation that PHAX is also accumulated in the same cytoplasmic accumulations suggests that SMNΔN27 interferes with proper Sm core assembly and blocks the release of the PHAX/CBC complex from the m7G cap structure. An interesting possibility is that the PHAX/CBC complex may protect the m7G cap structure until the Sm core is correctly assembled. Therefore, the formation of the snRNP core within the SMN complex may be necessary for the release of the PHAX/CBC complex from the m7G cap structure, making it accessible for hypermethylation.

The association of the SMN complex with cytoplasmic m3G-capped snRNAs indicates that the SMN complex is not released from the snRNP after Sm core assembly and is present during and after cap hypermethylation (Fig. 6). The snRNA-(guanosine-N2)-methyltransferase responsible for the snRNA m3G cap formation in yeast has recently been identified (47). It has been shown that the methyltransferase binds the Sm core directly through its interaction with SmB (47, 56). Our results raise the possibility that the SMN complex may help recruit the methyltransferase or play some role in its activity.

The third distinct snRNP-associated SMN complex detected in the cytoplasm contains snurportin1 (Fig. 6). The RNA-dependent coimmunoprecipitation of the SMN complex and snurportin1 indicates that the SMN complex remains bound to the snRNPs prior to their import. This observation raises the possibility that the SMN complex may be imported into the nucleus together with the newly assembled snRNPs. Indeed, the SMN complex is also found in the nucleus (38). It is conceivable that the SMN complex may play a direct role in snRNP import because at least one factor that interacts with the Sm core and plays a role in snRNP import remains unidentified (12, 59). If this hypothesis is correct, coilin may dissociate the SMN/snRNP complex in the Cajal bodies as previously suggested (26). Alternatively, the SMN complex may be released from the snRNPs immediately, prior to or coincident with their import via the nuclear pore complex. The binding of importin-β to snurportin1 may be the trigger to release the SMN complex from the snRNP core, and the SMN complex may be imported into the nucleus via a distinct pathway.

In conclusion, we showed that the SMN complex binds newly exported snRNAs and is associated with snRNPs during m3G cap formation and with an snRNP preimport complex containing snurportin1. Therefore, the SMN complex not only plays a role in snRNP core assembly but is an integral component of, and likely serves as a chaperone during, the entire snRNP core biogenesis process in the cytoplasm.

Acknowledgments

We thank R. Luhrmann for the anti-m3G cap structure antibody (R1131) and Eng Tan for the anti-p80 coilin antibody. We are grateful to members of our laboratory, in particular Zissimos Mourelatos, Jeongsik Yong, Naoyuki Kataoka, Josée Dostie, Amélie Gubitz, and Westley Friesen, for helpful discussions and comments on the manuscript.

This work was supported by the Association Française contre les Myopathies (AFM) and by a grant from the National Institutes of Health.

REFERENCES

  • 1.Brahms, H., L. Meheus, V. de Brabandere, U. Fischer, and R. Luhrmann. 2001. Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B′ and the Sm-like protein LSm4, and their interaction with the SMN protein. RNA 7:1531-1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Buhler, D., V. Raker, R. Luhrmann, and U. Fischer. 1999. Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy. Hum. Mol. Genet. 8:2351-2357. [DOI] [PubMed] [Google Scholar]
  • 3.Campbell, L., K. M. Hunter, P. Mohaghegh, J. M. Tinsley, M. A. Brasch, and K. E. Davies. 2000. Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation? Hum. Mol. Genet. 9:1093-1100. [DOI] [PubMed] [Google Scholar]
  • 4.Charroux, B., L. Pellizzoni, R. A. Perkinson, A. Shevchenko, M. Mann, and G. Dreyfuss. 1999. Gemin3: a novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. J. Cell Biol. 147:1181-1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Charroux, B., L. Pellizzoni, R. A. Perkinson, J. Yong, A. Shevchenko, M. Mann, and G. Dreyfuss. 2000. Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. J. Cell Biol. 148:1177-1186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Choi, Y. D., and G. Dreyfuss. 1984. Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells. J. Cell Biol. 99:1997-2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Coovert, D. D., T. T. Le, P. E. McAndrew, J. Strasswimmer, T. O. Crawford, J. R. Mendell, S. E. Coulson, E. J. Androphy, T. W. Prior, and A. H. Burghes. 1997. The survival motor neuron protein in spinal muscular atrophy. Hum. Mol. Genet. 6:1205-1214. [DOI] [PubMed] [Google Scholar]
  • 8.England, T. E., and O. C. Uhlenbeck. 1978. 3′-terminal labelling of RNA with T4 RNA ligase. Nature 275:560-561. [DOI] [PubMed] [Google Scholar]
  • 9.Fischer, U., J. Huber, W. C. Boelens, I. W. Mattaj, and R. Luhrmann. 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82:475-483. [DOI] [PubMed] [Google Scholar]
  • 10.Fischer, U., Q. Liu, and G. Dreyfuss. 1997. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis. Cell 90:1023-1029. [DOI] [PubMed] [Google Scholar]
  • 11.Fischer, U., and R. Luhrmann. 1990. An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus. Science 249:786-790. [DOI] [PubMed] [Google Scholar]
  • 12.Fischer, U., V. Sumpter, M. Sekine, T. Satoh, and R. Luhrmann. 1993. Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap. EMBO J. 12:573-583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Fornerod, M., M. Ohno, M. Yoshida, and I. W. Mattaj. 1997. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051-1060. [DOI] [PubMed] [Google Scholar]
  • 14.Frey, M. R., and A. G. Matera. 2001. RNA-mediated interaction of Cajal bodies and U2 snRNA genes. J. Cell Biol. 154:499-509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Friesen, W. J., and G. Dreyfuss. 2000. Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN). J. Biol. Chem. 275:26370-26375. [DOI] [PubMed] [Google Scholar]
  • 16.Friesen, W. J., S. Massenet, S. Paushkin, A. Wyce, and G. Dreyfuss. 2001. Smn, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets. Mol. Cell 7:1111-1117. [DOI] [PubMed] [Google Scholar]
  • 17.Friesen, W. J., S. Paushkin, A. Wyce, S. Massenet, G. S. Pesiridis, G. Van Duyn, J. Rappsilber, M. Mann, and G. Dreyfuss. 2001. The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins. Mol. Cell. Biol. 21:8289-8300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Friesen, W. J., A. Wyce, S. Paushkin, L. Abel, J. Rappsilber, M. Mann, and G. Dreyfuss. 2002. A novel WD repeat protein component of the methylosome binds Sm proteins. J. Biol. Chem. 277:8243-8247. [DOI] [PubMed] [Google Scholar]
  • 19.Frugier, T., F. D. Tiziano, C. Cifuentes-Diaz, P. Miniou, N. Roblot, A. Dierich, M. Le Meur, and J. Melki. 2000. Nuclear targeting defect of SMN lacking the C-terminus in a mouse model of spinal muscular atrophy. Hum. Mol. Genet. 9:849-858. [DOI] [PubMed] [Google Scholar]
  • 20.Gilliam, A. C., and J. A. Steitz. 1993. Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs. Proc. Natl. Acad. Sci. USA 90:6781-6785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Gorlach, M., C. G. Burd, and G. Dreyfuss. 1994. The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity. Exp. Cell. Res. 211:400-407. [DOI] [PubMed] [Google Scholar]
  • 22.Gorlich, D., R. Kraft, S. Kostka, F. Vogel, E. Hartmann, R. A. Laskey, I. W. Mattaj, and E. Izaurraide. 1996. Importin provides a link between nuclear protein import and U snRNA export. Cell 87:21-32. [DOI] [PubMed] [Google Scholar]
  • 23.Gubitz, A. K., Z. Mourelatos, L. Abel, J. Rappsilber, M. Mann, and G. Dreyfuss. 2002. Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. J. Biol. Chem. 277:5631-5636. [DOI] [PubMed] [Google Scholar]
  • 24.Hamm, J., E. Darzynkiewicz, S. M. Tahara, and I. W. Mattaj. 1990. The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal. Cell 62:569-577. [DOI] [PubMed] [Google Scholar]
  • 25.Hamm, J., and I. W. Mattaj. 1990. Monomethylated cap structures facilitate RNA export from the nucleus. Cell 63:109-118. [DOI] [PubMed] [Google Scholar]
  • 26.Hebert, M. D., P. W. Szymczyk, K. B. Shpargel, and A. G. Matera. 2001. Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein. Genes Dev. 15:2720-2729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Hsieh-Li, H. M., J. G. Chang, Y. J. Jong, M. H. Wu, N. M. Wang, C. H. Tsai, and H. Li. 2000. A mouse model for spinal muscular atrophy. Nat. Genet. 24:66-70. [DOI] [PubMed] [Google Scholar]
  • 28.Huber, J., U. Cronshagen, M. Kadokura, C. Marshallsay, T. Wada, M. Sekine, and R. Luhrmann. 1998. Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure. EMBO J. 17:4114-4126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Izaurralde, E., U. Kutay, C. von Kobbe, I. W. Mattaj, and D. Gorlich. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16:6535-6547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Izaurralde, E., J. Lewis, C. Gamberi, A. Jarmolowski, C. McGuigan, and I. W. Mattaj. 1995. A cap-binding protein complex mediating U snRNA export. Nature 376:709-712. [DOI] [PubMed] [Google Scholar]
  • 31.Izaurralde, E., J. Lewis, C. McGuigan, M. Jankowska, E. Darzynkiewicz, and I. W. Mattaj. 1994. A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell 78:657-668. [DOI] [PubMed] [Google Scholar]
  • 32.Jablonka, S., B. Schrank, M. Kralewski, W. Rossoll, and M. Sendtner. 2000. Reduced survival motor neuron (Smn) gene dose in mice leads to motor neuron degeneration: an animal model for spinal muscular atrophy type III. Hum. Mol. Genet. 9:341-346. [DOI] [PubMed] [Google Scholar]
  • 33.Jarmolowski, A., W. C. Boelens, E. Izaurralde, and I. W. Mattaj. 1994. Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol. 124:627-635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Kambach, C., S. Walke, R. Young, J. M. Avis, E. de la Fortelle, V. A. Raker, R. Luhrmann, J. Li, and K. Nagai. 1999. Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. Cell 96:375-387. [DOI] [PubMed] [Google Scholar]
  • 35.Kataoka, N., M. Ohno, K. Kangawa, Y. Tokoro, and Y. Shimura. 1994. Cloning of a complementary DNA encoding an 80 kilodalton nuclear cap binding protein. Nucleic Acids Res. 22:3861-3865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Kataoka, N., M. Ohno, I. Moda, and Y. Shimura. 1995. Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein. Nucleic Acids Res. 23:3638-3641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Lerner, M. R., and J. A. Steitz. 1979. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 76:5495-5499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Liu, Q., and G. Dreyfuss. 1996. A novel nuclear structure containing the survival of motor neurons protein. EMBO J. 15:3555-3565. [PMC free article] [PubMed] [Google Scholar]
  • 39.Liu, Q., U. Fischer, F. Wang, and G. Dreyfuss. 1997. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell 90:1013-1021. [DOI] [PubMed] [Google Scholar]
  • 40.Mattaj, I. W. 1986. Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding. Cell 46:905-911. [DOI] [PubMed] [Google Scholar]
  • 41.Mattaj, I. W., and E. M. De Robertis. 1985. Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins. Cell 40:111-118. [DOI] [PubMed] [Google Scholar]
  • 42.Meister, G., D. Buhler, B. Laggerbauer, M. Zobawa, F. Lottspeich, and U. Fischer. 2000. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum. Mol. Genet. 9:1977-1986. [DOI] [PubMed] [Google Scholar]
  • 43.Meister, G., D. Buhler, R. Pillai, F. Lottspeich, and U. Fisher. 2001. A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs. Nat. Cell Biol. 3:945-949. [DOI] [PubMed] [Google Scholar]
  • 44.Meister, G., C. Eggert, D. Buhler, H. Brahms, C. Kambach, and U. Fischer. 2001. Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln. Curr. Biol. 11:1990-1994. [DOI] [PubMed] [Google Scholar]
  • 45.Melki, J. 1997. Spinal muscular atrophy. Curr. Opin. Neurol. 10:381-385. [DOI] [PubMed] [Google Scholar]
  • 46.Monani, U. R., M. Sendtner, D. D. Coovert, D. W. Parsons, C. Andreassi, T. T. Le, S. Jablonka, B. Schrank, W. Rossol, T. W. Prior, G. E. Morris, and A. H. Burghes. 2000. The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(−/−) mice and results in a mouse with spinal muscular atrophy. Hum. Mol. Genet. 9:333-339. [DOI] [PubMed] [Google Scholar]
  • 47.Mouaikel, J., C. Verheggen, E. Bertrand, J. Tazi, and R. Bordonne. 2002. Hypermethylation of the Cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus. Mol. Cell 9:891-901. [DOI] [PubMed] [Google Scholar]
  • 48.Neuman de Vegvar, H. E., and J. E. Dahlberg. 1990. Nucleocytoplasmic transport and processing of small nuclear RNA precursors. Mol. Cell. Biol. 10:3365-3375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 49.Ohno, M., A. Segref, A. Bachi, M. Wilm, and I. W. Mattaj. 2000. PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation. Cell 101:187-198. [DOI] [PubMed] [Google Scholar]
  • 50.Palacios, I., M. Hetzer, S. A. Adam, and I. W. Mattaj. 1997. Nuclear import of U snRNPs requires importin beta. EMBO J. 16:6783-6792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Pellizzoni, L., J. Baccon, B. Charroux, and G. Dreyfuss. 2001. The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1. Curr. Biol. 11:1079-1088. [DOI] [PubMed] [Google Scholar]
  • 52.Pellizzoni, L., J. Baccon, J. Rappsilber, M. Mann, and G. Dreyfuss. 2002. Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. J. Biol. Chem. 277:7540-7545. [DOI] [PubMed] [Google Scholar]
  • 53.Pellizzoni, L., B. Charroux, and G. Dreyfuss. 1999. SMN mutants of spinal muscular atrophy patients are defective in binding to snRNP proteins. Proc. Natl. Acad. Sci. USA 96:11167-11172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 54.Pellizzoni, L., B. Charroux, J. Rappsilber, M. Mann, and G. Dreyfuss. 2001. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell Biol. 152:75-85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 55.Pellizzoni, L., N. Kataoka, B. Charroux, and G. Dreyfuss. 1998. A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing. Cell 95:615-624. [DOI] [PubMed] [Google Scholar]
  • 56.Plessel, G., U. Fischer, and R. Luhrmann. 1994. m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain. Mol. Cell. Biol. 14:4160-4172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 57.Segref, A., I. W. Mattaj, and M. Ohno. 2001. The evolutionarily conserved region of the U snRNA export mediator PHAX is a novel RNA-binding domain that is essential for U snRNA export. RNA 7:351-360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Siomi, M. C., P. S. Eder, N. Kataoka, L. Wan, Q. Liu, and G. Dreyfuss. 1997. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins. J. Cell Biol. 138:1181-1192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Will, C. L., and R. Luhrmann. 2001. Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell Biol. 13:290-301. [DOI] [PubMed] [Google Scholar]
  • 60.Yong, J., L. Pellizzoni, and G. Dreyfuss. 2002. Sequence-specific interaction of U1 snRNA with the SMN complex. EMBO J. 21:1188-1196. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES