Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1966 Mar;183(2):433–449. doi: 10.1113/jphysiol.1966.sp007875

On the permeability of the presynaptic terminal of the crayfish neuromuscular junction during synaptic inhibition and the action of γ-aminobutyric acid

A Takeuchi, Noriko Takeuchi
PMCID: PMC1357587  PMID: 16992221

Abstract

1. The contribution of chloride to presynaptic inhibition of the crayfish neuromuscular junction during the action of γ-aminobutyric acid (GABA) and the inhibitory transmitter was investigated. Chloride in van Harreveld's solution was replaced with propionate, acetate, methylsulphate or glycerophosphate and electrical changes were recorded intracellularly and extracellularly with micro-electrodes.

2. When the preparation was soaked in Cl-deficient solutions, the quantum content of the excitatory junctional potentials (e.j.p.s), calculated from the number of failures, was increased.

3. The presynaptic inhibitory action of GABA was reduced or almost absent when the preparation was soaked in Cl-deficient solution. In Cl-deficient solution the stimulation of the inhibitory axon showed little or no inhibitory action on e.j.p.s.

4. If GABA was applied shortly after the outside solution was changed to the Cl-deficient one, the frequency of the spontaneous e.j.p.s was increased transiently.

5. It is suggested that GABA or the inhibitory transmitter act on the presynaptic excitatory terminal and predominantly increase its permeability to chloride.

Full text

PDF
433

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOISTEL J., FATT P. Membrane permeability change during inhibitory transmitter action in crustacean muscle. J Physiol. 1958 Nov 10;144(1):176–191. doi: 10.1113/jphysiol.1958.sp006094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DUDEL J., KUFFLER S. W. Presynaptic inhibition at the crayfish neuromuscular junction. J Physiol. 1961 Mar;155:543–562. doi: 10.1113/jphysiol.1961.sp006646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUDEL J. PRESYNAPTIC AND POSTSYNAPTIC EFFECTS OF INHIBITORY DRUGS ON THE CRAYFISH NEUROMUSCULAR JUNCTION. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Mar 18;283:104–118. doi: 10.1007/BF00363182. [DOI] [PubMed] [Google Scholar]
  4. DUDEL J. PRESYNAPTIC INHIBITION OF THE EXCITATORY NERVE TERMINAL IN THE NEUROMUSCULAR JUNCTION OF THE CRAYFISH. Pflugers Arch Gesamte Physiol Menschen Tiere. 1963 Sep 9;277:537–557. [PubMed] [Google Scholar]
  5. DUDEL J. THE ACTION OF INHIBITORY DRUGS ON NERVE TERMINALS IN CRAYFISH MUSCLE. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 May 10;284:81–94. doi: 10.1007/BF00412369. [DOI] [PubMed] [Google Scholar]
  6. FALK G., LANDA J. F. Prolonged response of skeletal muscle in the absence of penetrating anions. Am J Physiol. 1960 Feb;198:289–299. doi: 10.1152/ajplegacy.1960.198.2.289. [DOI] [PubMed] [Google Scholar]
  7. FATT P., KATZ B. The effect of inhibitory nerve impulses on a crustacean muscle fibre. J Physiol. 1953 Aug;121(2):374–389. doi: 10.1113/jphysiol.1953.sp004952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIRARDIER L., REUBEN J. P., BRANDT P. W., GRUNDFEST H. EVIDENCE FOR ANION-PERMSELECTIVE MEMBRANE IN CRAYFISH MUSCLE FIBERS AND ITS POSSIBLE ROLE IN EXCITATION-CONTRACTION COUPLING. J Gen Physiol. 1963 Sep;47:189–214. doi: 10.1085/jgp.47.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GRUNDFEST H., REUBEN J. P., RICKLES W. H., Jr The electrophysiology and pharmacology of lobster neuromuscular synapses. J Gen Physiol. 1959 Jul 20;42(6):1301–1323. doi: 10.1085/jgp.42.6.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HAGIWARA S., KUSANO K., SAITO S. Membrane changes in crayfish stretch receptor neutron during synaptic inhibition and under action of gamma-aminobutyric acid. J Neurophysiol. 1960 Sep;23:505–515. doi: 10.1152/jn.1960.23.5.505. [DOI] [PubMed] [Google Scholar]
  11. HOROWICZ P. THE EFFECTS OF ANIONS ON EXCITABLE CELLS. Pharmacol Rev. 1964 Jun;16:193–221. [PubMed] [Google Scholar]
  12. HUTTER O. F., NOBLE D. The chloride conductance of frog skeletal muscle. J Physiol. 1960 Apr;151:89–102. [PMC free article] [PubMed] [Google Scholar]
  13. ITO M., KOSTYUK P. G., OSHIMA T. Further study on anion permeability of inhibitory post-synaptic membrane of cat motoneurones. J Physiol. 1962 Oct;164:150–156. doi: 10.1113/jphysiol.1962.sp007009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KATZ B., MILEDI R. A STUDY OF SPONTANEOUS MINIATURE POTENTIALS IN SPINAL MOTONEURONES. J Physiol. 1963 Sep;168:389–422. doi: 10.1113/jphysiol.1963.sp007199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ORKAND R. K. The relation between membrane potential and contraction in single crayfish muscle fibres. J Physiol. 1962 Apr;161:143–159. doi: 10.1113/jphysiol.1962.sp006878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TAKEUCHI A., TAKEUCHI N. LOCALIZED ACTION OF GAMMA-AMINOBUTYRIC ACID ON THE CRAYFISH MUSCLE. J Physiol. 1965 Mar;177:225–238. doi: 10.1113/jphysiol.1965.sp007588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takeuchi A., Takeuchi N. A study of the inhibitory action of gamma-amino-butyric acid on neuromuscular transmission in the crayfish. J Physiol. 1966 Mar;183(2):418–432. doi: 10.1113/jphysiol.1966.sp007874. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES