Abstract
1. The stop flow procedure was used to compare the urinary creatinine: inulin ratios in male and female rats; to confirm previous evidence for secretion of creatinine by the renal tubule in the male rat, and to assess the effect of androgenic hormones on this property of the renal tubule in the female rat.
2. The creatinine:inulin ratios in free flow and all stop flow samples in the female rat were close to unity, confirming the absence of secretion of creatinine.
3. In the male rat the ratios in the free flow samples exceeded 1·2. In samples proximal to the sodium minimum the ratio rose to a peak of 1·56, and then declined to the free flow level.
4. Female rats pre-treated with androgenic hormones exhibited a male type pattern. A single intramuscular dose of testosterone, or daily injections of progesterone for 1 month, had a similar effect. The free flow ratios rose to 1·2 and on stop flow a proximal secretory peak was seen.
5. Two conclusions may be drawn—the creatinine clearance is a valid measure of glomerular filtration rate in the female rat, but not in the male; and the secretion of creatinine is a function of the proximal tubular cells which can be influenced by androgenic hormones in the rat.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FINGL E. Tubular excretion of creatinine in the rat. Am J Physiol. 1952 May;169(2):357–362. doi: 10.1152/ajplegacy.1952.169.2.357. [DOI] [PubMed] [Google Scholar]
- GLASSER L. Renal excretion of creatinine in the rat. Am J Physiol. 1961 Jan;200:167–169. doi: 10.1152/ajplegacy.1961.200.1.167. [DOI] [PubMed] [Google Scholar]
- Harvey A. M., Malvin R. L. Comparison of creatinine and inulin clearances in male and female rats. Am J Physiol. 1965 Oct;209(4):849–852. doi: 10.1152/ajplegacy.1965.209.4.849. [DOI] [PubMed] [Google Scholar]
- MALVIN R. L. The stop flow method and its use in studying renal function. Prog Cardiovasc Dis. 1961 Mar;3:432–448. doi: 10.1016/s0033-0620(61)80002-9. [DOI] [PubMed] [Google Scholar]
- MALVIN R. L., WILDE W. S., SULLIVAN L. P. Localization of nephron transport by stop flow analysis. Am J Physiol. 1958 Jul;194(1):135–142. doi: 10.1152/ajplegacy.1958.194.1.135. [DOI] [PubMed] [Google Scholar]
- O'connor J. M., Conway E. J. The localisation of excretion in the uriniferous tubule. J Physiol. 1922 May 16;56(3-4):190–200. doi: 10.1113/jphysiol.1922.sp002001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SWANSON R. E., HAKIM A. A. Stop-flow analysis of creatinine excretion in the dog. Am J Physiol. 1962 Dec;203:980–984. doi: 10.1152/ajplegacy.1962.203.6.980. [DOI] [PubMed] [Google Scholar]
- WALSER M., DAVIDSON D. G., ORLOFF J. The renal clearance of alkali-stable inulin. J Clin Invest. 1955 Oct;34(10):1520–1523. doi: 10.1172/JCI103204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMSON H. E., SKULAN T. W., SHIDEMAN F. E. Effects of adrenalectomy and desoxycorticosterone on stop-flow patterns of sodium and potassium in the rat. J Pharmacol Exp Ther. 1961 Jan;131:49–55. [PubMed] [Google Scholar]
- YOUNG J. A., EDWARDS K. D. STOP-FLOW ANALYSIS OF RENAL TUBULAR FUNCTION IN THE RAT UNDERGOING OSMOTIC DIURESIS DUE TO CREATININE LOADING. Aust J Exp Biol Med Sci. 1964 Dec;42:667–688. doi: 10.1038/icb.1964.64. [DOI] [PubMed] [Google Scholar]