Skip to main content
PMC home page
Annals of Surgery logoLink to Annals of Surgery
. 1991 Jul;214(1):83–88. doi: 10.1097/00000658-199107000-00013

Anti-tumor effect of L-methionine-deprived total parenteral nutrition with 5-fluorouracil administration on Yoshida sarcoma-bearing rats.

N Goseki 1, M Endo 1, T Onodera 1, G Kosaki 1
PMCID: PMC1358418  PMID: 1905913

Abstract

L-methionine-deprived total parenteral nutrition (methionine-deprived TPN), infusing amino acid solution devoid of L-methionine and L-cysteine by the method of TPN as an only protein source, showed enhancement of the effect of several anti-cancer agents. In this study the combined effect of the methionine-deprived TPN with administration of 5-fluorouracil (5-FU) was examined in Yoshida Sarcoma (YS)-bearing rats, from aspects of effects on the tumor metastasis and the host animal's life span, in the following four groups treated with: methionine-deprived TPN with administration of 5-FU, methionine-deprived TPN without administration of 5-FU, L-methionine-contained TPN plus 5-FU, and L-methionine-contained TPN without 5-FU. In the first experiment, TPN was continued for 8 days in the four groups, and the anti-cancer effect of methionine-deprived TPN and administration of 5-FU based on both the growth of the primary tumor at the implanted site and the tumor metastasis was studied from the view point of pathologic findings of animals killed immediately after these treatments. In experiment 2 the survival period was examined after these treatments for 10 days with subsequent oral feeding until death. The results were as follows: proliferation of YS, transplanted subcutaneously, was markedly suppressed; particularly hematogenous metastasis, characteristic in YS, was prominently blunted then obtained an apparent longer survival period in rats treated with the methionine-deprived TPN with administration of 5-FU.

Full text

PDF
83

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashe H., Clark B. R., Chu F., Hardy D. N., Halpern B. C., Halpern R. M., Smith R. A. N5-methyltetrahydrofolate: homocysteine methyltransferase activity in extracts from normal, malignant and embryonic tissue culture cells. Biochem Biophys Res Commun. 1974 Mar 25;57(2):417–425. doi: 10.1016/0006-291x(74)90947-4. [DOI] [PubMed] [Google Scholar]
  2. Breillout F., Hadida F., Echinard-Garin P., Lascaux V., Poupon M. F. Decreased rat rhabdomyosarcoma pulmonary metastases in response to a low methionine diet. Anticancer Res. 1987 Jul-Aug;7(4B):861–867. [PubMed] [Google Scholar]
  3. Chello P. L., Bertino J. R. Effect of methionine deprivation on L5178Y murine leukemia cells in culture interference with the antineoplastic effect of methotrexate. Biochem Pharmacol. 1976 Apr 15;25(8):889–892. doi: 10.1016/0006-2952(76)90309-9. [DOI] [PubMed] [Google Scholar]
  4. Goseki N., Endo M., Onodera T., Kosaki G. Influence of L-methionine-deprived total parenteral nutrition on the tumor tissue and plasma amino acids fraction and the host metabolism: experimental study with Sato lung carcinoma-bearing rats. Tohoku J Exp Med. 1989 Mar;157(3):251–260. doi: 10.1620/tjem.157.251. [DOI] [PubMed] [Google Scholar]
  5. Goseki N., Endo M. Thiol depletion and chemosensitization on nimustine hydrochloride by methionine-depleting total parenteral nutrition. Tohoku J Exp Med. 1990 Jul;161(3):227–239. doi: 10.1620/tjem.161.227. [DOI] [PubMed] [Google Scholar]
  6. Goseki N., Mori S., Habu H., Menju M., Murakami T. [Effect of intravenous methionine-free hyperalimentation combined with anti-cancer drugs (RT-therapy) on adenocarcinoma of gastrointestinal tract (author's transl)]. Nihon Shokakibyo Gakkai Zasshi. 1980 Jan;77(1):112–112. [PubMed] [Google Scholar]
  7. Goseki N., Onodera T., Koike M., Kosaki G. Inhibitory effect of L-methionine-deprived amino acid imbalance using total parenteral nutrition on growth of ascites hepatoma in rats. Tohoku J Exp Med. 1987 Feb;151(2):191–200. doi: 10.1620/tjem.151.191. [DOI] [PubMed] [Google Scholar]
  8. Goseki N., Onodera T., Mori S., Menjo M. [Clinical study of amino acid imbalance as an adjunct to cancer therapy]. Nihon Gan Chiryo Gakkai Shi. 1982 Oct 20;17(7):1908–1916. [PubMed] [Google Scholar]
  9. Hoffman R. M., Jacobsen S. J., Erbe R. W. Reversion to methionine independence by malignant rat and SV40-transformed human fibroblasts. Biochem Biophys Res Commun. 1978 May 15;82(1):228–234. doi: 10.1016/0006-291x(78)90600-9. [DOI] [PubMed] [Google Scholar]
  10. Kreis W., Hession C. Biological effects of enzymatic deprivation of L-methionine in cell culture and an experimental tumor. Cancer Res. 1973 Aug;33(8):1866–1869. [PubMed] [Google Scholar]
  11. Kreis W. Tumor therapy by deprivation of L-methionine: rationale and results. Cancer Treat Rep. 1979 Jun;63(6):1069–1072. [PubMed] [Google Scholar]
  12. Steiger E., Vars H. M., Dudrick S. J. A technique for long-term intravenous feeding in unrestrained rats. Arch Surg. 1972 Mar;104(3):330–332. doi: 10.1001/archsurg.1972.04180030076017. [DOI] [PubMed] [Google Scholar]

Articles from Annals of Surgery are provided here courtesy of Lippincott, Williams, and Wilkins

RESOURCES