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. 1997 Nov;92(3):317–320. doi: 10.1046/j.1365-2567.1997.00361.x

Circulating immune complexes from HIV-1+ patients induces apoptosis on normal lymphocytes.

E Aceituno 1, S Castañón 1, C Jiménez 1, D Subirá 1, M De Górgolas 1, M Fernández-Guerrero 1, F Ortíz 1, R García 1
PMCID: PMC1363790  PMID: 9486102

Abstract

Isolated immune complexes from sera of 49 out of 67 human immunodeficiency virus-1-positive (HIV-1+) patients (CIC-HIV+), composed of anti-HIV-HIV-Ag, could induce apoptosis on normal phytohaemagglutinin (PHA)-activated lymphocytes. DNA degradation was detected by propidium iodide staining. This activity is directed against CD4+ lymphocytes as demonstrated by double binding of CIC-HIV+ and anti-CD4 on apoptosis cells. Expression of Fas antigen is prior to apoptotic phenomena. CIC-HIV+ apoptosis inducers belong mainly to asymptomatic HIV-infected patients, indicating that immune complexes from these patients can destroy CD4+ lymphocytes.

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Selected References

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  1. Amadori A., De Silvestro G., Zamarchi R., Veronese M. L., Mazza M. R., Schiavo G., Panozzo M., De Rossi A., Ometto L., Mous J. CD4 epitope masking by gp120/anti-gp120 antibody complexes. A potential mechanism for CD4+ cell function down-regulation in AIDS patients. J Immunol. 1992 May 1;148(9):2709–2716. [PubMed] [Google Scholar]
  2. Banda N. K., Bernier J., Kurahara D. K., Kurrle R., Haigwood N., Sekaly R. P., Finkel T. H. Crosslinking CD4 by human immunodeficiency virus gp120 primes T cells for activation-induced apoptosis. J Exp Med. 1992 Oct 1;176(4):1099–1106. doi: 10.1084/jem.176.4.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carbonari M., Cibati M., Cherchi M., Sbarigia D., Pesce A. M., Dell'Anna L., Modica A., Fiorilli M. Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method. Blood. 1994 Mar 1;83(5):1268–1277. [PubMed] [Google Scholar]
  4. Euler H. H., Kern P., Löffler H., Dietrich M. Precipitable immune complexes in healthy homosexual men, acquired immune deficiency syndrome and the related lymphadenopathy syndrome. Clin Exp Immunol. 1985 Feb;59(2):267–275. [PMC free article] [PubMed] [Google Scholar]
  5. Feijoó E., Subirá D., Fernández-Guerrero M., García R., Ortíz F. Anti-CD4 activity in circulating immune complexes in HIV-infected patients. Int Arch Allergy Immunol. 1995 Apr;106(4):366–371. doi: 10.1159/000236868. [DOI] [PubMed] [Google Scholar]
  6. Finkel T. H., Tudor-Williams G., Banda N. K., Cotton M. F., Curiel T., Monks C., Baba T. W., Ruprecht R. M., Kupfer A. Apoptosis occurs predominantly in bystander cells and not in productively infected cells of HIV- and SIV-infected lymph nodes. Nat Med. 1995 Feb;1(2):129–134. doi: 10.1038/nm0295-129. [DOI] [PubMed] [Google Scholar]
  7. García R., Feijoó E., Guerrero M. F., de Górgolas M., Munoz-Fernández M. A., Fernández-Cruz E., Ortíz F. Immune complexes from HIV-1+ patients contain infectious virus able to infect normal lymphocytes. J Allergy Clin Immunol. 1996 Oct;98(4):827–830. doi: 10.1016/s0091-6749(96)70132-x. [DOI] [PubMed] [Google Scholar]
  8. Groux H., Torpier G., Monté D., Mouton Y., Capron A., Ameisen J. C. Activation-induced death by apoptosis in CD4+ T cells from human immunodeficiency virus-infected asymptomatic individuals. J Exp Med. 1992 Feb 1;175(2):331–340. doi: 10.1084/jem.175.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meyaard L., Otto S. A., Jonker R. R., Mijnster M. J., Keet R. P., Miedema F. Programmed death of T cells in HIV-1 infection. Science. 1992 Jul 10;257(5067):217–219. doi: 10.1126/science.1352911. [DOI] [PubMed] [Google Scholar]
  10. Nicoletti I., Migliorati G., Pagliacci M. C., Grignani F., Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271–279. doi: 10.1016/0022-1759(91)90198-o. [DOI] [PubMed] [Google Scholar]
  11. Ormerod M. G., Sun X. M., Brown D., Snowden R. T., Cohen G. M. Quantification of apoptosis and necrosis by flow cytometry. Acta Oncol. 1993;32(4):417–424. doi: 10.3109/02841869309093620. [DOI] [PubMed] [Google Scholar]
  12. Oyaizu N., McCloskey T. W., Coronesi M., Chirmule N., Kalyanaraman V. S., Pahwa S. Accelerated apoptosis in peripheral blood mononuclear cells (PBMCs) from human immunodeficiency virus type-1 infected patients and in CD4 cross-linked PBMCs from normal individuals. Blood. 1993 Dec 1;82(11):3392–3400. [PubMed] [Google Scholar]
  13. Oyaizu N., Pahwa S. Role of apoptosis in HIV disease pathogenesis. J Clin Immunol. 1995 Sep;15(5):217–231. doi: 10.1007/BF01540879. [DOI] [PubMed] [Google Scholar]
  14. Raff M. C. Social controls on cell survival and cell death. Nature. 1992 Apr 2;356(6368):397–400. doi: 10.1038/356397a0. [DOI] [PubMed] [Google Scholar]
  15. Sekigawa I., Koshino K., Hishikawa T., Kaneko H., Takasaki Y., Hashimoto H., Hirose S., Inagaki Y., Yamamoto N. Inhibitory effect of the immunosuppressant FK506 on apoptotic cell death induced by HIV-1 gp120. J Clin Immunol. 1995 Nov;15(6):312–317. doi: 10.1007/BF01541321. [DOI] [PubMed] [Google Scholar]
  16. Wang Z. Q., Orlikowsky T., Dudhane A., Mittler R., Blum M., Lacy E., Riethmüller G., Hoffmann M. K. Deletion of T lymphocytes in human CD4 transgenic mice induced by HIV-gp120 and gp120-specific antibodies from AIDS patients. Eur J Immunol. 1994 Jul;24(7):1553–1557. doi: 10.1002/eji.1830240715. [DOI] [PubMed] [Google Scholar]

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