Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1997 Nov;92(3):340–345. doi: 10.1046/j.1365-2567.1997.00350.x

Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution.

N D Machugh 1, J K Mburu 1, M J Carol 1, C R Wyatt 1, J A Orden 1, W C Davis 1
PMCID: PMC1363794  PMID: 9486106

Abstract

We describe the characterization of two subsets of bovine gamma delta T cells having distinct cell surface phenotype and tissue distribution. One population expresses the previously described 215,000 MW WC1 antigen and is negative for the cell-surface differentiation antigens CD2, CD4, and CD8. The second population expresses CD2 and CD8 but not WC1 and appears to have a T-cell receptor (TCR) rearrangement distinct from that of the WC1+ population. The WC1- population is found in large numbers in spleen and intestine. In addition, this subset is not recognized by a number of monoclonal antibodies (mAbs) specific for TCR families that are well represented in the WC1+ population. The results indicate that the gamma delta T-cell population in cattle is considerably larger than previously described and that this population can be subdivided into two distinct subsets based on cell-surface phenotype and tissue distribution.

Full text

PDF
340

Images in this article

343Figure 3
on p.343
343Figure 4
on p.343
343Figure 5
on p.343

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bensaid A., Hadam M. Individual antigens of cattle. Bovine CD4 (BoCD4). Vet Immunol Immunopathol. 1991 Jan;27(1-3):51–54. doi: 10.1016/0165-2427(91)90078-q. [DOI] [PubMed] [Google Scholar]
  2. Brenner M. B., McLean J., Scheft H., Riberdy J., Ang S. L., Seidman J. G., Devlin P., Krangel M. S. Two forms of the T-cell receptor gamma protein found on peripheral blood cytotoxic T lymphocytes. Nature. 1987 Feb 19;325(6106):689–694. doi: 10.1038/325689a0. [DOI] [PubMed] [Google Scholar]
  3. Clevers H., MacHugh N. D., Bensaid A., Dunlap S., Baldwin C. L., Kaushal A., Iams K., Howard C. J., Morrison W. I. Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes. Eur J Immunol. 1990 Apr;20(4):809–817. doi: 10.1002/eji.1830200415. [DOI] [PubMed] [Google Scholar]
  4. Davis W. C., Ellis J. A., MacHugh N. D., Baldwin C. L. Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2. Immunology. 1988 Jan;63(1):165–167. [PMC free article] [PubMed] [Google Scholar]
  5. Davodeau F., Peyrat M. A., Houde I., Hallet M. M., De Libero G., Vié H., Bonneville M. Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science. 1993 Jun 18;260(5115):1800–1802. doi: 10.1126/science.8390096. [DOI] [PubMed] [Google Scholar]
  6. Ellis J. A., Davis W. C., MacHugh N. D., Emery D. L., Kaushal A., Morrison W. I. Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies. Vet Immunol Immunopathol. 1988 Oct;19(3-4):325–340. doi: 10.1016/0165-2427(88)90118-3. [DOI] [PubMed] [Google Scholar]
  7. Faure F., Jitsukawa S., Miossec C., Hercend T. CD1c as a target recognition structure for human T lymphocytes: analysis with peripheral blood gamma/delta cells. Eur J Immunol. 1990 Mar;20(3):703–706. doi: 10.1002/eji.1830200336. [DOI] [PubMed] [Google Scholar]
  8. Goddeeris B. M., Baldwin C. L., ole-MoiYoi O., Morrison W. I. Improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells. Functional evaluation of monocytes in responses to lectins. J Immunol Methods. 1986 May 22;89(2):165–173. doi: 10.1016/0022-1759(86)90354-6. [DOI] [PubMed] [Google Scholar]
  9. Groh V., Porcelli S., Fabbi M., Lanier L. L., Picker L. J., Anderson T., Warnke R. A., Bhan A. K., Strominger J. L., Brenner M. B. Human lymphocytes bearing T cell receptor gamma/delta are phenotypically diverse and evenly distributed throughout the lymphoid system. J Exp Med. 1989 Apr 1;169(4):1277–1294. doi: 10.1084/jem.169.4.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hochstenbach F., Parker C., McLean J., Gieselmann V., Band H., Bank I., Chess L., Spits H., Strominger J. L., Seidman J. G. Characterization of a third form of the human T cell receptor gamma/delta. J Exp Med. 1988 Aug 1;168(2):761–776. doi: 10.1084/jem.168.2.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Littman D. R., Newton M., Crommie D., Ang S. L., Seidman J. G., Gettner S. N., Weiss A. Characterization of an expressed CD3-associated Ti gamma-chain reveals C gamma domain polymorphism. Nature. 1987 Mar 5;326(6108):85–88. doi: 10.1038/326085a0. [DOI] [PubMed] [Google Scholar]
  12. MacHugh N. D., Bensaid A., Davis W. C., Howard C. J., Parsons K. R., Jones B., Kaushal A. Characterization of a bovine thymic differentiation antigen analogous to CD1 in the human. Scand J Immunol. 1988 May;27(5):541–547. doi: 10.1111/j.1365-3083.1988.tb02381.x. [DOI] [PubMed] [Google Scholar]
  13. MacHugh N. D., Bensaid A., Howard C. J., Davis W. C., Morrison W. I. Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8. Vet Immunol Immunopathol. 1991 Jan;27(1-3):169–172. doi: 10.1016/0165-2427(91)90096-u. [DOI] [PubMed] [Google Scholar]
  14. Mackay C. R., Hein W. R. A large proportion of bovine T cells express the gamma delta T cell receptor and show a distinct tissue distribution and surface phenotype. Int Immunol. 1989;1(5):540–545. doi: 10.1093/intimm/1.5.540. [DOI] [PubMed] [Google Scholar]
  15. Mackay C. R., Maddox J. F., Brandon M. R. Three distinct subpopulations of sheep T lymphocytes. Eur J Immunol. 1986 Jan;16(1):19–25. doi: 10.1002/eji.1830160105. [DOI] [PubMed] [Google Scholar]
  16. Matis L. A., Fry A. M., Cron R. Q., Cotterman M. M., Dick R. F., Bluestone J. A. Structure and specificity of a class II MHC alloreactive gamma delta T cell receptor heterodimer. Science. 1989 Aug 18;245(4919):746–749. doi: 10.1126/science.2528206. [DOI] [PubMed] [Google Scholar]
  17. Mellon P., Parker V., Gluzman Y., Maniatis T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell. 1981 Dec;27(2 Pt 1):279–288. doi: 10.1016/0092-8674(81)90411-6. [DOI] [PubMed] [Google Scholar]
  18. Morrison W. I., Davis W. C. Individual antigens of cattle. Differentiation antigens expressed predominantly on CD4- CD8- T lymphocytes (WC1, WC2). Vet Immunol Immunopathol. 1991 Jan;27(1-3):71–76. doi: 10.1016/0165-2427(91)90082-n. [DOI] [PubMed] [Google Scholar]
  19. Naessens J., Newson J., Williams D. J., Lutje V. Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies. Immunology. 1988 Apr;63(4):569–574. [PMC free article] [PubMed] [Google Scholar]
  20. Oettgen H. C., Pettey C. L., Maloy W. L., Terhorst C. A T3-like protein complex associated with the antigen receptor on murine T cells. Nature. 1986 Mar 20;320(6059):272–275. doi: 10.1038/320272a0. [DOI] [PubMed] [Google Scholar]
  21. Porcelli S., Brenner M. B., Greenstein J. L., Balk S. P., Terhorst C., Bleicher P. A. Recognition of cluster of differentiation 1 antigens by human CD4-CD8-cytolytic T lymphocytes. Nature. 1989 Oct 5;341(6241):447–450. doi: 10.1038/341447a0. [DOI] [PubMed] [Google Scholar]
  22. Rivas A., Koide J., Cleary M. L., Engleman E. G. Evidence for involvement of the gamma, delta T cell antigen receptor in cytotoxicity mediated by human alloantigen-specific T cell clones. J Immunol. 1989 Mar 15;142(6):1840–1846. [PubMed] [Google Scholar]
  23. Sopp P., Howard C. J., Parsons K. R. Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence. Vet Immunol Immunopathol. 1991 Jan;27(1-3):163–168. doi: 10.1016/0165-2427(91)90095-t. [DOI] [PubMed] [Google Scholar]
  24. Spits H., Paliard X., Engelhard V. H., de Vries J. E. Cytotoxic activity and lymphokine production of T cell receptor (TCR)-alpha beta+ and TCR-gamma delta+ cytotoxic T lymphocyte (CTL) clones recognizing HLA-A2 and HLA-A2 mutants. Recognition of TCR-gamma delta+ CTL clones is affected by mutations at positions 152 and 156. J Immunol. 1990 Jun 1;144(11):4156–4162. [PubMed] [Google Scholar]
  25. Wijngaard P. L., MacHugh N. D., Metzelaar M. J., Romberg S., Bensaid A., Pepin L., Davis W. C., Clevers H. C. Members of the novel WC1 gene family are differentially expressed on subsets of bovine CD4-CD8- gamma delta T lymphocytes. J Immunol. 1994 Apr 1;152(7):3476–3482. [PubMed] [Google Scholar]
  26. Wu Y. J., Tian W. T., Snider R. M., Rittershaus C., Rogers P., LaManna L., Ip S. H. Signal transduction of gamma/delta T cell antigen receptor with a novel mitogenic anti-delta antibody. J Immunol. 1988 Sep 1;141(5):1476–1479. [PubMed] [Google Scholar]
  27. Wyatt C. R., Madruga C., Cluff C., Parish S., Hamilton M. J., Goff W., Davis W. C. Differential distribution of gamma delta T-cell receptor lymphocyte subpopulations in blood and spleen of young and adult cattle. Vet Immunol Immunopathol. 1994 Mar;40(3):187–199. doi: 10.1016/0165-2427(94)90019-1. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES