Gene	Primer Pair	Annealing Temp (ºC)	Expected Band Sizes (bps)
vit-1(ok2616)	For: ATCAGTTGTCCGCGGAATTGRev: GGGACATTGGCTCAACTGTG	59	WT: 2399MT: 602
vit-1(ok2616)	For: CATCCTCCCAGTCGATACCCRev: GGGACATTGGCTCAACTGTG	59	WT: 1445MT: 0
vit-2(ok3211)	For: TCACATGGAAAACGAGGACARev: TGGGATCTTTCCAGAGATGG	59	WT: 1321MT: 600
vit-3(rhd320)	For: TCCGCTTTTTGCAAAGTATCRev: TGGTTGACGTGGATCTTGGA	56	- EcoRI: 845+ EcoRI: 386 & 409
vit-4(rhd321)	For: TACTTTCAGGTCTCTGGACCRev: TTTGTCTAGATGCTGGGCGG	57	- EcoRI: 599+ EcoRI: 342 & 257
vit-5(rhd322)	For: CAGCACACAAGTTTTCAGGTRev: GATGCTCTTCTTCTCGAAGT	56	- EcoRI: 361+ EcoRI: 351 & 10
vit-6(rhd332)	For: CGCACCCTCGAAGGAGAATGRev: CAAGAGATGGGTAGCGCATG	59	- EcoRI: 820+ EcoRI: 405 & 415