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. 1997 Dec;92(4):553–560. doi: 10.1046/j.1365-2567.1997.00368.x

Characterization of adhesive interactions between mast cells and laminin isoforms: evidence of a principal role for alpha 6 integrin.

H Vliagoftis 1, D D Metcalfe 1
PMCID: PMC1364162  PMID: 9497498

Abstract

Mast cells are known to adhere to laminin, although there is limited information on the characteristics of this event. To further examine this adhesive interaction, we thus determined the adherence of murine mast cell lines and primary bone marrow cultured mast cells (BMCMC) to murine laminin (mLN), human placental laminin-1 (hLN), merosin (laminin-2) and various laminin fragments, concentrating on activating stimuli, the involvement of integrins, and the effect on mast cell activation. Murine mast cells were found to adhere to both mLN and hLN and to merosin, not only following exposure to phorbol 12-myristate 13-acetate (PMA), but also after Fc epsilon RI aggregation or addition of stem cell factor (SCF). Adhesion to laminin was partially inhibited by soluble E8 and PA22-2, both fragments of laminin that promote mast-cell adhesion when bound on surfaces. Mast-cell lines and BMCMC consistently expressed high levels of alpha 6 integrin. Antibody to alpha 6 blocked spontaneous and inhibited activated mast-cell adhesion to hLN, and inhibited mast-cell adhesion to mLN and its fragment E8. Mast-cell adhesion to both laminin isoforms increased Fc epsilon RI-mediated mast-cell secretion. These observations demonstrate that mast-cell attachment to laminin is promoted by physiological stimuli, is mediated principally by alpha 6 integrin, and results in enhanced cell activation.

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Selected References

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  1. Aumailley M., Timpl R., Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp Cell Res. 1990 May;188(1):55–60. doi: 10.1016/0014-4827(90)90277-h. [DOI] [PubMed] [Google Scholar]
  2. Bianchine P. J., Burd P. R., Metcalfe D. D. IL-3-dependent mast cells attach to plate-bound vitronectin. Demonstration of augmented proliferation in response to signals transduced via cell surface vitronectin receptors. J Immunol. 1992 Dec 1;149(11):3665–3671. [PubMed] [Google Scholar]
  3. Bohnsack J. F. CD11/CD18-independent neutrophil adherence to laminin is mediated by the integrin VLA-6. Blood. 1992 Mar 15;79(6):1545–1552. [PubMed] [Google Scholar]
  4. Chang A. C., Salomon D. R., Wadsworth S., Hong M. J., Mojcik C. F., Otto S., Shevach E. M., Coligan J. E. Alpha 3 beta 1 and alpha 6 beta 1 integrins mediate laminin/merosin binding and function as costimulatory molecules for human thymocyte proliferation. J Immunol. 1995 Jan 15;154(2):500–510. [PubMed] [Google Scholar]
  5. Clément B., Segui-Real B., Savagner P., Kleinman H. K., Yamada Y. Hepatocyte attachment to laminin is mediated through multiple receptors. J Cell Biol. 1990 Jan;110(1):185–192. doi: 10.1083/jcb.110.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooper H. M., Tamura R. N., Quaranta V. The major laminin receptor of mouse embryonic stem cells is a novel isoform of the alpha 6 beta 1 integrin. J Cell Biol. 1991 Nov;115(3):843–850. doi: 10.1083/jcb.115.3.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dastych J., Costa J. J., Thompson H. L., Metcalfe D. D. Mast cell adhesion to fibronectin. Immunology. 1991 Aug;73(4):478–484. [PMC free article] [PubMed] [Google Scholar]
  8. Dastych J., Metcalfe D. D. Stem cell factor induces mast cell adhesion to fibronectin. J Immunol. 1994 Jan 1;152(1):213–219. [PubMed] [Google Scholar]
  9. Delwel G. O., de Melker A. A., Hogervorst F., Jaspars L. H., Fles D. L., Kuikman I., Lindblom A., Paulsson M., Timpl R., Sonnenberg A. Distinct and overlapping ligand specificities of the alpha 3A beta 1 and alpha 6A beta 1 integrins: recognition of laminin isoforms. Mol Biol Cell. 1994 Feb;5(2):203–215. doi: 10.1091/mbc.5.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Georas S. N., McIntyre B. W., Ebisawa M., Bednarczyk J. L., Sterbinsky S. A., Schleimer R. P., Bochner B. S. Expression of a functional laminin receptor (alpha 6 beta 1, very late activation antigen-6) on human eosinophils. Blood. 1993 Nov 1;82(9):2872–2879. [PubMed] [Google Scholar]
  11. Gismondi A., Mainiero F., Morrone S., Palmieri G., Piccoli M., Frati L., Santoni A. Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6. J Exp Med. 1992 Nov 1;176(5):1251–1257. doi: 10.1084/jem.176.5.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Graf J., Ogle R. C., Robey F. A., Sasaki M., Martin G. R., Yamada Y., Kleinman H. K. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987 Nov 3;26(22):6896–6900. doi: 10.1021/bi00396a004. [DOI] [PubMed] [Google Scholar]
  13. Grant D. S., Tashiro K., Segui-Real B., Yamada Y., Martin G. R., Kleinman H. K. Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell. 1989 Sep 8;58(5):933–943. doi: 10.1016/0092-8674(89)90945-8. [DOI] [PubMed] [Google Scholar]
  14. Hamawy M. M., Oliver C., Mergenhagen S. E., Siraganian R. P. Adherence of rat basophilic leukemia (RBL-2H3) cells to fibronectin-coated surfaces enhances secretion. J Immunol. 1992 Jul 15;149(2):615–621. [PubMed] [Google Scholar]
  15. Huhtala P., Humphries M. J., McCarthy J. B., Tremble P. M., Werb Z., Damsky C. H. Cooperative signaling by alpha 5 beta 1 and alpha 4 beta 1 integrins regulates metalloproteinase gene expression in fibroblasts adhering to fibronectin. J Cell Biol. 1995 May;129(3):867–879. doi: 10.1083/jcb.129.3.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lin T. H., Yurochko A., Kornberg L., Morris J., Walker J. J., Haskill S., Juliano R. L. The role of protein tyrosine phosphorylation in integrin-mediated gene induction in monocytes. J Cell Biol. 1994 Sep;126(6):1585–1593. doi: 10.1083/jcb.126.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rao N. C., Barsky S. H., Terranova V. P., Liotta L. A. Isolation of a tumor cell laminin receptor. Biochem Biophys Res Commun. 1983 Mar 29;111(3):804–808. doi: 10.1016/0006-291x(83)91370-0. [DOI] [PubMed] [Google Scholar]
  19. Schwartz L. B., Austen K. F., Wasserman S. I. Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol. 1979 Oct;123(4):1445–1450. [PubMed] [Google Scholar]
  20. Shaw L. M., Messier J. M., Mercurio A. M. The activation dependent adhesion of macrophages to laminin involves cytoskeletal anchoring and phosphorylation of the alpha 6 beta 1 integrin. J Cell Biol. 1990 Jun;110(6):2167–2174. doi: 10.1083/jcb.110.6.2167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shimizu Y., Irani A. M., Brown E. J., Ashman L. K., Schwartz L. B. Human mast cells derived from fetal liver cells cultured with stem cell factor express a functional CD51/CD61 (alpha v beta 3) integrin. Blood. 1995 Aug 1;86(3):930–939. [PubMed] [Google Scholar]
  22. Thompson H. L., Burbelo P. D., Metcalfe D. D. Regulation of adhesion of mouse bone marrow-derived mast cells to laminin. J Immunol. 1990 Nov 15;145(10):3425–3431. [PubMed] [Google Scholar]
  23. Thompson H. L., Burbelo P. D., Segui-Real B., Yamada Y., Metcalfe D. D. Laminin promotes mast cell attachment. J Immunol. 1989 Oct 1;143(7):2323–2327. [PubMed] [Google Scholar]
  24. Thompson H. L., Burbelo P. D., Yamada Y., Kleinman H. K., Metcalfe D. D. Identification of an amino acid sequence in the laminin A chain mediating mast cell attachment and spreading. Immunology. 1991 Jan;72(1):144–149. [PMC free article] [PubMed] [Google Scholar]
  25. Walsh L. J., Kaminer M. S., Lazarus G. S., Lavker R. M., Murphy G. F. Role of laminin in localization of human dermal mast cells. Lab Invest. 1991 Oct;65(4):433–440. [PubMed] [Google Scholar]
  26. Yasuda M., Hasunuma Y., Adachi H., Sekine C., Sakanishi T., Hashimoto H., Ra C., Yagita H., Okumura K. Expression and function of fibronectin binding integrins on rat mast cells. Int Immunol. 1995 Feb;7(2):251–258. doi: 10.1093/intimm/7.2.251. [DOI] [PubMed] [Google Scholar]
  27. Zhang Z., Vuori K., Reed J. C., Ruoslahti E. The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6161–6165. doi: 10.1073/pnas.92.13.6161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van de Wiel-van Kemenade E., van Kooyk Y., de Boer A. J., Huijbens R. J., Weder P., van de Kasteele W., Melief C. J., Figdor C. G. Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. J Cell Biol. 1992 Apr;117(2):461–470. doi: 10.1083/jcb.117.2.461. [DOI] [PMC free article] [PubMed] [Google Scholar]

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