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. 1998 Jun;94(2):173–180. doi: 10.1046/j.1365-2567.1998.00502.x

Activation-dependent expression of the insulin-like growth factor binding protein-2 in human lymphocytes.

J L Föll 1, L Dannecker 1, C Zehrer 1, S Hettmer 1, J Berger 1, M Elmlinger 1, D Niethammer 1, M B Ranke 1, G E Dannecker 1
PMCID: PMC1364202  PMID: 9741338

Abstract

The expression of the insulin-like growth factor binding protein-2 (IGFBP-2) was assayed in mononuclear cells originating from different organs of the immune system. All mononuclear cells studied did express IGFBP-2, but the expression level was found to be dependent on the cell type and origin of the cell. T cells showed a higher expression of IGFBP-2 mRNA than did B cells, and CD34+ stem cells expressed IGFBP-2 mRNA at a high level. Expression was highest in bone marrow and thymus. Stimulation of peripheral mononuclear cells resulted in a marked increase of IGFBP-2 mRNA and also intracellular IGFBP-2, as analysed by fluorescence staining. This increase parallels the increase of other known T-cell activation markers. Furthermore, the increase of intracellular IGFBP-2 seems to precede T-cell blast formation and all T cells in active phases of the cell cycle have high levels of IGFBP-2. Our results provide a basis for further investigations on the contribution of the IGF-system to the regulation of T-cell proliferation and differentiation. IGFBP-2, in particular, may have an important influence in the regulation of T-cell activation and proliferation.

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