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. 1998 Jun;94(2):228–234. doi: 10.1046/j.1365-2567.1998.00511.x

Prostaglandin and fatty acid modulation of Escherichia coli O157 phagocytosis by human monocytic cells.

J Davidson 1, A Kerr 1, K Guy 1, D Rotondo 1
PMCID: PMC1364209  PMID: 9741345

Abstract

Phagocytosis by human monocytes is an important primary survival mechanism particularly during bacterial infection. However, the processes that control the events and mediators involved in the activation of monocytes and their impact on the phagocytosis of bacteria are poorly understood. The effect of bacterial endotoxin, interleukin-1 beta (IL-1 beta), fatty acids and prostaglandin E2 (PGE2) on the phagocytosis of fluoroscein isothiocyanate (FITC)-labelled Escherichia coli (O157) by human blood monocytes and U937 cells was studied by flow cytometry. Endotoxin increased the phagocytosis of labelled bacteria by both monocytes and U937 cells. IL-1 beta and the polyunsaturated fatty acids; dihomo-gamma-linolenic and arachidonic acids also increased the phagocytic activity of both monocytes and U937 cells. In contrast, PGE2 suppressed phagocytosis in a concentration-dependent manner. The cyclo-oxygenase inhibitor, ketoprofen, further enhanced the increased phagocytic activity in the presence of endotoxin and interleukin-1 (IL-1) indicating suppression by endogenous prostaglandins. This was confirmed by the data which showed that lipopolysaccharide (LPS) and IL-1 increased PGE2 release and ketoprofen inhibited release. Endotoxin and fatty acids increased IL-1 beta release also, whereas PGE2 inhibited release. The data suggest that phagocytic activity may be linked to changes in IL-1 levels. The data presented in this study also suggest that monocyte phagocytosis in the course of bacterial infection would be altered during pathophysiological events which result in elevation of extracellular fatty acids.

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Selected References

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  1. Baldie G., Kaimakamis D., Rotondo D. Fatty acid modulation of cytokine release from human monocytic cells. Biochim Biophys Acta. 1993 Nov 7;1179(2):125–133. doi: 10.1016/0167-4889(93)90133-a. [DOI] [PubMed] [Google Scholar]
  2. Dinarello C. A., Ikejima T., Warner S. J., Orencole S. F., Lonnemann G., Cannon J. G., Libby P. Interleukin 1 induces interleukin 1. I. Induction of circulating interleukin 1 in rabbits in vivo and in human mononuclear cells in vitro. J Immunol. 1987 Sep 15;139(6):1902–1910. [PubMed] [Google Scholar]
  3. Gelfand J. A., Fauci A. S., Green I., Frank M. M. A simple method for the determination of complement receptor-bearing mononuclear cells. J Immunol. 1976 Mar;116(3):595–599. [PubMed] [Google Scholar]
  4. Ghezzi P., Dinarello C. A. IL-1 induces IL-1. III. Specific inhibition of IL-1 production by IFN-gamma. J Immunol. 1988 Jun 15;140(12):4238–4244. [PubMed] [Google Scholar]
  5. Lennartz M. R., Brown E. J. Arachidonic acid is essential for IgG Fc receptor-mediated phagocytosis by human monocytes. J Immunol. 1991 Jul 15;147(2):621–626. [PubMed] [Google Scholar]
  6. Rietschel E. T., Kirikae T., Schade F. U., Mamat U., Schmidt G., Loppnow H., Ulmer A. J., Zähringer U., Seydel U., Di Padova F. Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB J. 1994 Feb;8(2):217–225. doi: 10.1096/fasebj.8.2.8119492. [DOI] [PubMed] [Google Scholar]
  7. Rotondo D., Abul H. T., Milton A. S., Davidson J. Pyrogenic immunomodulators increase the level of prostaglandin E2 in the blood simultaneously with the onset of fever. Eur J Pharmacol. 1988 Sep 13;154(2):145–152. doi: 10.1016/0014-2999(88)90091-x. [DOI] [PubMed] [Google Scholar]
  8. Rotondo D., Earl C. R., McIntosh G., McIntosh F. S., Hepburn A., Milton A. S., Davidson J. The role of inositol lipids in the activation of monocytes by interleukin-1 and bacterial endotoxin. FEMS Microbiol Immunol. 1992 Dec;5(5-6):249–259. doi: 10.1111/j.1574-6968.1992.tb05909.x. [DOI] [PubMed] [Google Scholar]
  9. Simms H. H., Gaither T. A., Fries L. F., Frank M. M. Monokines released during short-term Fc gamma receptor phagocytosis up-regulate polymorphonuclear leukocytes and monocyte-phagocytic function. J Immunol. 1991 Jul 1;147(1):265–272. [PubMed] [Google Scholar]
  10. Thore M., Löfgren S., Tärnvik A., Monsen T., Selstam E., Burman L. G. Anaerobic phagocytosis, killing, and degradation of Streptococcus pneumoniae by human peripheral blood leukocytes. Infect Immun. 1985 Jan;47(1):277–281. doi: 10.1128/iai.47.1.277-281.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Warner S. J., Auger K. R., Libby P. Interleukin 1 induces interleukin 1. II. Recombinant human interleukin 1 induces interleukin 1 production by adult human vascular endothelial cells. J Immunol. 1987 Sep 15;139(6):1911–1917. [PubMed] [Google Scholar]
  12. Watson D. A., Musher D. M., Jacobson J. W., Verhoef J. A brief history of the pneumococcus in biomedical research: a panoply of scientific discovery. Clin Infect Dis. 1993 Nov;17(5):913–924. doi: 10.1093/clinids/17.5.913. [DOI] [PubMed] [Google Scholar]

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