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. 1998 Jul;94(3):368–375. doi: 10.1046/j.1365-2567.1998.00497.x

Expression of CD69 activation marker by endometrial granulated lymphocytes throughout the menstrual cycle and in early pregnancy.

N Vassiliadou 1, J N Bulmer 1
PMCID: PMC1364255  PMID: 9767419

Abstract

Flow cytometry studies of first-trimester normal human decidua have proposed that a proportion of endometrial granulated lymphocytes (eGL), the predominant leucocyte population in early human pregnancy, co-express CD69. The purpose of this study was to investigate CD69 expression by immunohistochemistry throughout the menstrual cycle and in first-trimester human decidua and to determine whether CD69 expression by eGL is an in vivo characteristic or whether the antigen is acquired in vitro after tissue disaggregation and cell purification. Single immunoenzymatic and double immunofluorescence labelling of tissue sections, supplemented with double immunoenzymatic staining of purified eGL and flow cytometry, indicated that eGL do not co-express CD69 in situ but that they acquire the molecule in vitro after cell purification and culture.

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Selected References

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  1. Bulmer J. N., Johnson P. M. The T-lymphocyte population in first-trimester human decidua does not express the interleukin-2 receptor. Immunology. 1986 Aug;58(4):685–687. [PMC free article] [PubMed] [Google Scholar]
  2. Bulmer J. N., Morrison L., Longfellow M., Ritson A., Pace D. Granulated lymphocytes in human endometrium: histochemical and immunohistochemical studies. Hum Reprod. 1991 Jul;6(6):791–798. doi: 10.1093/oxfordjournals.humrep.a137430. [DOI] [PubMed] [Google Scholar]
  3. Bulmer J. N., Sunderland C. A. Immunohistological characterization of lymphoid cell populations in the early human placental bed. Immunology. 1984 Jun;52(2):349–357. [PMC free article] [PubMed] [Google Scholar]
  4. Chen C. K., Huang S. C., Chen C. L., Yen M. R., Hsu H. C., Ho H. N. Increased expressions of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of nonpregnant women. Hum Immunol. 1995 Mar;42(3):227–232. doi: 10.1016/0198-8859(94)00105-y. [DOI] [PubMed] [Google Scholar]
  5. Daya S., Clark D. A., Devlin C., Jarrell J., Chaput A. Suppressor cells in human decidua. Am J Obstet Gynecol. 1985 Jan 15;151(2):267–270. doi: 10.1016/0002-9378(85)90024-9. [DOI] [PubMed] [Google Scholar]
  6. Dudley D. J., Trautman M. S., Araneo B. A., Edwin S. S., Mitchell M. D. Decidual cell biosynthesis of interleukin-6: regulation by inflammatory cytokines. J Clin Endocrinol Metab. 1992 Apr;74(4):884–889. doi: 10.1210/jcem.74.4.1548355. [DOI] [PubMed] [Google Scholar]
  7. Ho H. N., Chao K. H., Chen C. K., Yang Y. S., Huang S. C. Activation status of T and NK cells in the endometrium throughout menstrual cycle and normal and abnormal early pregnancy. Hum Immunol. 1996 Sep 1;49(2):130–136. doi: 10.1016/0198-8859(96)00120-6. [DOI] [PubMed] [Google Scholar]
  8. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  9. Jones R. K., Bulmer J. N., Searle R. F. Cytotoxic activity of endometrial granulated lymphocytes during the menstrual cycle in humans. Biol Reprod. 1997 Nov;57(5):1217–1222. doi: 10.1095/biolreprod57.5.1217. [DOI] [PubMed] [Google Scholar]
  10. King A., Balendran N., Wooding P., Carter N. P., Loke Y. W. CD3- leukocytes present in the human uterus during early placentation: phenotypic and morphologic characterization of the CD56++ population. Dev Immunol. 1991;1(3):169–190. doi: 10.1155/1991/83493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. King A., Wheeler R., Carter N. P., Francis D. P., Loke Y. W. The response of human decidual leukocytes to IL-2. Cell Immunol. 1992 May;141(2):409–421. doi: 10.1016/0008-8749(92)90159-m. [DOI] [PubMed] [Google Scholar]
  12. Klentzeris L. D., Bulmer J. N., Warren M. A., Morrison L., Li T. C., Cooke I. D. Lymphoid tissue in the endometrium of women with unexplained infertility: morphometric and immunohistochemical aspects. Hum Reprod. 1994 Apr;9(4):646–652. doi: 10.1093/oxfordjournals.humrep.a138564. [DOI] [PubMed] [Google Scholar]
  13. Lanier L. L., Buck D. W., Rhodes L., Ding A., Evans E., Barney C., Phillips J. H. Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen. J Exp Med. 1988 May 1;167(5):1572–1585. doi: 10.1084/jem.167.5.1572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marshall R. J., Jones D. B. An immunohistochemical study of lymphoid tissue in human endometrium. Int J Gynecol Pathol. 1988;7(3):225–235. doi: 10.1097/00004347-198809000-00003. [DOI] [PubMed] [Google Scholar]
  15. Maruyama T., Makino T., Sugi T., Matsubayashi H., Ozawa N., Nozawa S. Flow-cytometric analysis of immune cell populations in human decidua from various types of first-trimester pregnancy. Hum Immunol. 1992 Jul;34(3):212–218. doi: 10.1016/0198-8859(92)90114-3. [DOI] [PubMed] [Google Scholar]
  16. Nishikawa K., Saito S., Morii T., Hamada K., Ako H., Narita N., Ichijo M., Kurahayashi M., Sugamura K. Accumulation of CD16-CD56+ natural killer cells with high affinity interleukin 2 receptors in human early pregnancy decidua. Int Immunol. 1991 Aug;3(8):743–750. doi: 10.1093/intimm/3.8.743. [DOI] [PubMed] [Google Scholar]
  17. Risso A., Cosulich M. E., Rubartelli A., Mazza M. R., Bargellesi A. MLR3 molecule is an activation antigen shared by human B, T lymphocytes and T cell precursors. Eur J Immunol. 1989 Feb;19(2):323–328. doi: 10.1002/eji.1830190216. [DOI] [PubMed] [Google Scholar]
  18. Ritson A., Bulmer J. N. Endometrial granulocytes in human decidua react with a natural-killer (NK) cell marker, NKH1. Immunology. 1987 Oct;62(2):329–331. [PMC free article] [PubMed] [Google Scholar]
  19. Ritson A., Bulmer J. N. Isolation and functional studies of granulated lymphocytes in first trimester human decidua. Clin Exp Immunol. 1989 Aug;77(2):263–268. [PMC free article] [PubMed] [Google Scholar]
  20. Saito S., Morii T., Enomoto M., Sakakura S., Nishikawa K., Narita N., Ichijo M. The effect of interleukin 2 and transforming growth factor-beta 2 (TGF-beta 2) on the proliferation and natural killer activity of decidual CD16- CD56bright natural killer cells. Cell Immunol. 1993 Dec;152(2):605–613. doi: 10.1006/cimm.1993.1316. [DOI] [PubMed] [Google Scholar]
  21. Saito S., Nishikawa K., Morii T., Narita N., Enomoto M., Ichijo M. Expression of activation antigens CD69, HLA-DR, interleukin-2 receptor-alpha (IL-2R alpha) and IL-2R beta on T cells of human decidua at an early stage of pregnancy. Immunology. 1992 Apr;75(4):710–712. [PMC free article] [PubMed] [Google Scholar]
  22. Starkey P. M. Expression on cells of early human pregnancy decidua, of the p75, IL-2 and p145, IL-4 receptor proteins. Immunology. 1991 May;73(1):64–70. [PMC free article] [PubMed] [Google Scholar]
  23. Starkey P. M., Sargent I. L., Redman C. W. Cell populations in human early pregnancy decidua: characterization and isolation of large granular lymphocytes by flow cytometry. Immunology. 1988 Sep;65(1):129–134. [PMC free article] [PubMed] [Google Scholar]
  24. Testi R., D'Ambrosio D., De Maria R., Santoni A. The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells. Immunol Today. 1994 Oct;15(10):479–483. doi: 10.1016/0167-5699(94)90193-7. [DOI] [PubMed] [Google Scholar]
  25. Testi R., Phillips J. H., Lanier L. L. Leu 23 induction as an early marker of functional CD3/T cell antigen receptor triggering. Requirement for receptor cross-linking, prolonged elevation of intracellular [Ca++] and stimulation of protein kinase C. J Immunol. 1989 Mar 15;142(6):1854–1860. [PubMed] [Google Scholar]
  26. Vassiliadou N., Bulmer J. N. Quantitative analysis of T lymphocyte subsets in pregnant and nonpregnant human endometrium. Biol Reprod. 1996 Nov;55(5):1017–1022. doi: 10.1095/biolreprod55.5.1017. [DOI] [PubMed] [Google Scholar]

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