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. 1998 May;94(1):94–100. doi: 10.1046/j.1365-2567.1998.00076.x

Variance in the resistance of murine early bone marrow B cells to a deficiency in zinc.

F Osati-Ashtiani 1, L E King 1, P J Fraker 1
PMCID: PMC1364336  PMID: 9708192

Abstract

Little is known of the effects of nutritional deficiencies on lymphopoietic processes. Nevertheless, deficiencies in zinc adversely affect immune function causing thymic atrophy and lymphopenia in both humans and animals. Previous studies of the effects of zinc deficiency (ZD) on lymphopoiesis in adult mice indicated that a suboptimal intake of zinc caused a 50% or more depletion of the marrow of developing B cells. Thus, interference in the production of lymphocytes by ZD appeared to be a significant factor in the loss of host defence capacity. In the current study three-colour immunofluorescence phenotyping of early bone marrow B lymphocytes (B220+ immunoglobulin-) using flow cytometry demonstrated that a 27-day period of ZD caused a 50-70% decline in pre-B cells (B220+ CD43- immunoglobulin M (IgM)-) for moderate and severely zinc-deficient mice, respectively. Conversely, early pro-B cells (B220+ CD43+ 6C3-) and late pro-B cells (B220+ CD43+ 6C3+) exhibited little or no change in their distribution within the marrow. Indeed, the greater resistance of pro-B cells resulted in a 50% increase in the proportion of this subset within the B-cell compartment of the marrow as the deficiency in zinc advanced. Collectively, the data indicate that the B-cell compartment of the marrow is substantially altered by ZD and the stage specific sensitivity noted among early B cells may be related to chronically elevated levels of glucocorticoids present during ZD or other parameters that affect their survival and resistance to apoptosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chesters J. K. Trace element-gene interactions. Nutr Rev. 1992 Aug;50(8):217–223. doi: 10.1111/j.1753-4887.1992.tb01331.x. [DOI] [PubMed] [Google Scholar]
  2. Cook-Mills J. M., Fraker P. J. Functional capacity of the residual lymphocytes from zinc-deficient adult mice. Br J Nutr. 1993 May;69(3):835–848. doi: 10.1079/bjn19930084. [DOI] [PubMed] [Google Scholar]
  3. DePasquale-Jardieu P., Fraker P. J. Further characterization of the role of corticosterone in the loss of humoral immunity in zinc-deficient A/J mice as determined by adrenalectomy. J Immunol. 1980 Jun;124(6):2650–2655. [PubMed] [Google Scholar]
  4. Fleming T. J., Fleming M. L., Malek T. R. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399–2408. [PubMed] [Google Scholar]
  5. Garvy B. A., King L. E., Telford W. G., Morford L. A., Fraker P. J. Chronic elevation of plasma corticosterone causes reductions in the number of cycling cells of the B lineage in murine bone marrow and induces apoptosis. Immunology. 1993 Dec;80(4):587–592. [PMC free article] [PubMed] [Google Scholar]
  6. Hardy R. R., Carmack C. E., Shinton S. A., Kemp J. D., Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med. 1991 May 1;173(5):1213–1225. doi: 10.1084/jem.173.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. King L. E., Fraker P. J. Flow cytometric analysis of the phenotypic distribution of splenic lymphocytes in zinc-deficient adult mice. J Nutr. 1991 Sep;121(9):1433–1438. doi: 10.1093/jn/121.9.1433. [DOI] [PubMed] [Google Scholar]
  8. King L. E., Osati-Ashtiani F., Fraker P. J. Depletion of cells of the B lineage in the bone marrow of zinc-deficient mice. Immunology. 1995 May;85(1):69–73. [PMC free article] [PubMed] [Google Scholar]
  9. Li Y. S., Hayakawa K., Hardy R. R. The regulated expression of B lineage associated genes during B cell differentiation in bone marrow and fetal liver. J Exp Med. 1993 Sep 1;178(3):951–960. doi: 10.1084/jem.178.3.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liles W. C., Dale D. C., Klebanoff S. J. Glucocorticoids inhibit apoptosis of human neutrophils. Blood. 1995 Oct 15;86(8):3181–3188. [PubMed] [Google Scholar]
  11. Merino R., Ding L., Veis D. J., Korsmeyer S. J., Nuñez G. Developmental regulation of the Bcl-2 protein and susceptibility to cell death in B lymphocytes. EMBO J. 1994 Feb 1;13(3):683–691. doi: 10.1002/j.1460-2075.1994.tb06307.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nixon D. E., Moyer T. P., Johnson P., McCall J. T., Ness A. B., Fjerstad W. H., Wehde M. B. Routine measurement of calcium, magnesium, copper, zinc, and iron in urine and serum by inductively coupled plasma emission spectroscopy. Clin Chem. 1986 Sep;32(9):1660–1665. [PubMed] [Google Scholar]
  13. Osmond D. G., Rico-Vargas S., Valenzona H., Fauteux L., Liu L., Janani R., Lu L., Jacobsen K. Apoptosis and macrophage-mediated cell deletion in the regulation of B lymphopoiesis in mouse bone marrow. Immunol Rev. 1994 Dec;142:209–230. doi: 10.1111/j.1600-065x.1994.tb00891.x. [DOI] [PubMed] [Google Scholar]
  14. Vallee B. L., Falchuk K. H. The biochemical basis of zinc physiology. Physiol Rev. 1993 Jan;73(1):79–118. doi: 10.1152/physrev.1993.73.1.79. [DOI] [PubMed] [Google Scholar]

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