Skip to main content
The Journal of Physiology logoLink to The Journal of Physiology
. 1968 Oct;198(3):613–626. doi: 10.1113/jphysiol.1968.sp008629

Assay of gastrin by means of its gastric acid stimulating activity

E L Blair, R M Keenlyside, D J Newell, J D Reed, Diana D Richardson
PMCID: PMC1365285  PMID: 5685291

Abstract

1. A method is described for the bio-assay of gastrin activity in the anaesthetized cat, based on the acid stimulating activity of gastrin.

2. A `gastrin' of unknown potency can be assayed in terms of a stable Standard Gastrin Extract or of Synthetic Human Gastrin I.

3. The sensitivity of the method is such that it will detect quantities of Synthetic Human Gastrin I at least as small as those at present described as detectable by radioimmunological techniques.

4. The precision of the assay is such that it falls in the category that has been described as very precise and well suited to clinical studies.

Full text

PDF
613

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beacham J., Bentley P. H., Gregory R. A., Kenner G. W., MacLeod J. K., Sheppard R. C. Synthesis of human gastrin I. Nature. 1966 Feb 5;209(5023):585–586. doi: 10.1038/209585a0. [DOI] [PubMed] [Google Scholar]
  2. LAI S. K. STUDIES ON GASTRIN. Gut. 1964 Aug;5:327–341. doi: 10.1136/gut.5.4.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. McGuigan J. E. Antibodies to the carboxyl-terminal tetrapeptide of gastrin. Gastroenterology. 1967 Nov;53(5):697–705. [PubMed] [Google Scholar]
  4. Stremple J. F., Abramoff P., van Oss C. J., Wilson S. D., Ellison E. H. Antibodies to synthetic human gastrin I. Lancet. 1967 Dec 2;2(7527):1180–1182. doi: 10.1016/s0140-6736(67)91895-8. [DOI] [PubMed] [Google Scholar]
  5. UVNAS B., EMAS S. A method for biologic assay of gastrin. Gastroenterology. 1961 May;40:644–648. [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES