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. 1968 Nov;199(1):37–57. doi: 10.1113/jphysiol.1968.sp008638

The metabolism of chylomicron fatty acids by isolated rat heart-lung preparations

M W Simpson-Morgan
PMCID: PMC1365343  PMID: 5684037

Abstract

1. An isolated rat heart—lung preparation is described. This preparation is perfused with the rat's own blood, in the absence of heparin or other anticoagulants, by the sustained efficient contractions of the heart. The preparation has only a small circulating blood volume and is stable for up to 3 hr.

2. Chylomicrons which were infused continuously into the circulating blood of the heart—lung preparation were removed from the circulation.

3. [14C]Palmitic acid incorporated into the chylomicrons was oxidized rapidly to CO2. Rates of oxidation of chylomicron fatty acids in excess of 3 mg/hr were measured and suggest that in the intact rat, the heart and lungs could account for up to 10% of the chylomicron fatty acids oxidized.

4. The infusion of glucose did not reduce the oxidation of the chylomicron fatty acids, whilst the infusion of sufficient quantities of chylomicrons in the absence of glucose spared some of the cardiac glycogen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLARD F. B., DANFORTH W. H., NAEGLE S., BING R. J. Myocardial metabolism of fatty acids. J Clin Invest. 1960 May;39:717–723. doi: 10.1172/JCI104088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRAGDON J. H., GORDON R. S., Jr Tissue distribution of C14 after the intravenous injection of labeled chylomicrons and unesterified fatty acids in the rat. J Clin Invest. 1958 Apr;37(4):574–578. doi: 10.1172/JCI103640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARKE E. W. A simplified heart-oxygenator preparation suitable for isotope experiments; with some observations on the metabolism of acetate, pyruvate and four amino acids. J Physiol. 1957 Apr 30;136(2):380–392. doi: 10.1113/jphysiol.1957.sp005767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cruickshank E. W., Kosterlitz H. W. The utilization of fat by the aglycaemic mammalian heart. J Physiol. 1941 Jan 14;99(2):208–223. doi: 10.1113/jphysiol.1941.sp003894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOLE V. P., HAMLIN J. T., 3rd Particulate fat in lymph and blood. Physiol Rev. 1962 Oct;42:674–701. doi: 10.1152/physrev.1962.42.4.674. [DOI] [PubMed] [Google Scholar]
  6. Delcher H. K., Fried M., Shipp J. C. Metabolism of lipoprotein lipid in the isolated perfused rat heart. Biochim Biophys Acta. 1965 Jul 7;106(1):10–18. doi: 10.1016/0005-2760(65)90090-1. [DOI] [PubMed] [Google Scholar]
  7. EVANS J. R., OPIE L. H., SHIPP J. C. METABOLISM OF PALMITIC ACID IN PERFUSED RAT HEART. Am J Physiol. 1963 Oct;205:766–770. doi: 10.1152/ajplegacy.1963.205.4.766. [DOI] [PubMed] [Google Scholar]
  8. Enser M. B., Kunz F., Borensztajn J., Opie L. H., Robinson D. S. Metabolism of triglyceride fatty acid by the perfused rat heart. Biochem J. 1967 Jul;104(1):306–317. doi: 10.1042/bj1040306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FRENCH J. E., MORRIS B. The tissue distribution and oxidation of 14C-labelled chylomicron fat injected intravenously in rats. J Physiol. 1958 Feb 17;140(2):262–271. doi: 10.1113/jphysiol.1958.sp005932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GOUSIOS A., FELTS J. M., HAVEL R. J. The metabolism of serum triglycerides and free fatty acids by the myocardium. Metabolism. 1963 Jan;12:75–80. [PubMed] [Google Scholar]
  11. Kreisberg R. A. Effect of diabetes and starvation on myocardial triglyceride and free fatty acid utilization. Am J Physiol. 1966 Feb;210(2):379–384. doi: 10.1152/ajplegacy.1966.210.2.379. [DOI] [PubMed] [Google Scholar]
  12. MORRIS B., FRENCH J. E. The uptake and metabolism of 14C labelled chylomicron fat by the isolated perfused liver of the rat. Q J Exp Physiol Cogn Med Sci. 1958 Apr;43(2):180–188. doi: 10.1113/expphysiol.1958.sp001315. [DOI] [PubMed] [Google Scholar]
  13. MORRIS B., SIMPSON-MORGAN M. W. EFFECTS OF GLUCOSE ON THE METABOLISM OF INFUSED CHYLOMICRON FATTY ACIDS IN UNANESTHETIZED RATS. J Physiol. 1965 Mar;177:74–93. doi: 10.1113/jphysiol.1965.sp007576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MORRIS B., SIMPSON-MORGAN M. W. THE EXCRETION OF 14CO2 DURING THE CONTINUOUS INTRAVENOUS INFUSION OF NAH-14CO3 IN UNANAESTHETIZED RATS. J Physiol. 1963 Dec;169:713–728. doi: 10.1113/jphysiol.1963.sp007291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MORRIS B., SIMPSON-MORGAN M. W. THE OXIDATION OF CONTINUOUSLY INFUSED CHYLOMICRON FATTY ACIDS BY UNANESTHETIZED RATS. J Physiol. 1963 Dec;169:729–742. doi: 10.1113/jphysiol.1963.sp007292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MORRIS B. THE METABOLISM OF FREE FATTY ACIDS AND CHYLOMICRON TRIGLYCERIDES BY THE ISOLATED PERFUSED LIVER OF THE RAT. J Physiol. 1963 Oct;168:564–583. doi: 10.1113/jphysiol.1963.sp007208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROBINSON D. S. THE CLEARING FACTOR LIPASE AND ITS ACTION IN THE TRANSPORT OF FATTY ACIDS BETWEEN THE BLOOD AND TISSUES. Adv Lipid Res. 1963;1:133–182. doi: 10.1016/b978-1-4831-9937-5.50010-7. [DOI] [PubMed] [Google Scholar]
  18. SIMPSON-MORGAN M. W., MORRIS B. A comparison of the metabolism of palmitic, oleic and linoleic acids incorporated into chylomicron triglycerides. Aust J Exp Biol Med Sci. 1962 Dec;40:445–453. doi: 10.1038/icb.1962.49. [DOI] [PubMed] [Google Scholar]
  19. STERN I., SHAPIRO B. A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood. J Clin Pathol. 1953 May;6(2):158–160. doi: 10.1136/jcp.6.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scheuer J., Olson R. E. Metabolism of exogenous triglyceride by the isolated perfused rat heart. Am J Physiol. 1967 Feb;212(2):301–307. doi: 10.1152/ajplegacy.1967.212.2.301. [DOI] [PubMed] [Google Scholar]
  21. VOLK M. E., MILLINGTON R. H., WEINHOUSE S. Oxidation of endogenous fatty acids of rat tissues in vitro. J Biol Chem. 1952 Apr;195(2):493–501. [PubMed] [Google Scholar]
  22. WILLIAMSON J. R., KREBS H. A. Acetoacetate as fuel of respiration in the perfused rat heart. Biochem J. 1961 Sep;80:540–547. doi: 10.1042/bj0800540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. ZACHARIAH P. Contractility and sugar permeability in the perfused rat heart. J Physiol. 1961 Sep;158:59–72. doi: 10.1113/jphysiol.1961.sp006754. [DOI] [PMC free article] [PubMed] [Google Scholar]

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