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. 1968 Nov;199(1):205–221. doi: 10.1113/jphysiol.1968.sp008649

Miniature synaptic potentials at frog spinal neurones in the presence of tetrodotoxin

F Colomo, S D Erulkar
PMCID: PMC1365354  PMID: 5684035

Abstract

1. Spontaneous subthreshold potentials have been recorded with an intracellular electrode from neurones of the isolated spinal cord of the frog.

2. Records of both depolarizing and hyperpolarizing potentials were obtained from cells in normal Ringer solution and also when impulse conduction in the cord had been abolished by tetrodotoxin (TTX).

3. Comparisons of results before and after TTX show that the majority of the spontaneous potentials are analogous to miniature end-plate potentials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARAKI T., OTANI T., FURUKAWA T. The electrical activities of single motoneurones in toad's spinal cord, recorded with intracellular electrodes. Jpn J Physiol. 1953 Dec 15;3(4):254–267. doi: 10.2170/jjphysiol.3.254. [DOI] [PubMed] [Google Scholar]
  2. BLACKMAN J. G., GINSBORG B. L., RAY C. Spontaneous synaptic activity in sympathetic ganglion cells of the frog. J Physiol. 1963 Jul;167:389–401. doi: 10.1113/jphysiol.1963.sp007157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOYD I. A., MARTIN A. R. Spontaneous subthreshold activity at mammalian neural muscular junctions. J Physiol. 1956 Apr 27;132(1):61–73. doi: 10.1113/jphysiol.1956.sp005502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BROCK L. G., COOMBS J. S., ECCLES J. C. The recording of potentials from motoneurones with an intracellular electrode. J Physiol. 1952 Aug;117(4):431–460. doi: 10.1113/jphysiol.1952.sp004759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BURNSTOCK G., HOLMAN M. E. Spontaneous potential at sympathetic nerve endings in smooth muscle. J Physiol. 1962 Mar;160:446–460. doi: 10.1113/jphysiol.1962.sp006858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Blankenship J. E. Action of tetrodotoxin on spinal motoneurons of the cat. J Neurophysiol. 1968 Mar;31(2):186–194. doi: 10.1152/jn.1968.31.2.186. [DOI] [PubMed] [Google Scholar]
  7. Blankenship J. E., Kuno M. Analysis of spontaneous subthreshold activity in spinal motoneurons of the cat. J Neurophysiol. 1968 Mar;31(2):195–209. doi: 10.1152/jn.1968.31.2.195. [DOI] [PubMed] [Google Scholar]
  8. DUDEL J., KUFFLER S. W. The quantal nature of transmission and spontaneous miniature potentials at the crayfish neuromuscular junction. J Physiol. 1961 Mar;155:514–529. doi: 10.1113/jphysiol.1961.sp006644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DUDEL J., ORKAND R. K. Spontaneous potential changes at crayfish neuromuscular junctions. Nature. 1960 May 7;186:476–477. doi: 10.1038/186476a0. [DOI] [PubMed] [Google Scholar]
  10. ECCLES J. C., ECCLES R. M., IGGO A., LUNDBERG A. Electrophysiological investigations on Renshaw cells. J Physiol. 1961 Dec;159:461–478. doi: 10.1113/jphysiol.1961.sp006821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ECCLES J. C. The mechanism of synaptic transmission. Ergeb Physiol. 1961;51:299–430. [PubMed] [Google Scholar]
  12. Elmqvist D., Feldman D. S. Spontaneous activity at a mammalian neuromuscular junction in tetrodotoxin. Acta Physiol Scand. 1965 Aug;64(4):475–476. doi: 10.1111/j.1748-1716.1965.tb04206.x. [DOI] [PubMed] [Google Scholar]
  13. FATT P., KATZ B. Some observations on biological noise. Nature. 1950 Oct 7;166(4223):597–598. doi: 10.1038/166597a0. [DOI] [PubMed] [Google Scholar]
  14. FATT P., KATZ B. Spontaneous subthreshold activity at motor nerve endings. J Physiol. 1952 May;117(1):109–128. [PMC free article] [PubMed] [Google Scholar]
  15. FURUKAWA T., SASAOKA T., HOSOYA Y. Effects of tetrodotoxin on the neuromuscular junction. Jpn J Physiol. 1959 Jun 25;9(2):143–152. doi: 10.2170/jjphysiol.9.143. [DOI] [PubMed] [Google Scholar]
  16. Hubbard J. I., Stenhouse D., Eccles R. M. Origin of synaptic noise. Science. 1967 Jul 21;157(3786):330–331. doi: 10.1126/science.157.3786.330. [DOI] [PubMed] [Google Scholar]
  17. KATZ B., MILEDI R. A STUDY OF SPONTANEOUS MINIATURE POTENTIALS IN SPINAL MOTONEURONES. J Physiol. 1963 Sep;168:389–422. doi: 10.1113/jphysiol.1963.sp007199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KATZ B., THESLEFF S. On the factors which determine the amplitude of the miniature end-plate potential. J Physiol. 1957 Jul 11;137(2):267–278. doi: 10.1113/jphysiol.1957.sp005811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KOLMODIN G. M., SKOGLUND C. R. Slow membrane potential changes accompanying excitation and inhibition in spinal moto- and interneurons in the cat during natural activation. Acta Physiol Scand. 1958 Oct 28;44(1):11–54. doi: 10.1111/j.1748-1716.1958.tb01607.x. [DOI] [PubMed] [Google Scholar]
  20. KUNO M. Effects of strychnine on the intracellular potentials of spinal motoneurons of the toad. Jpn J Physiol. 1957 Mar 15;7(1):42–50. doi: 10.2170/jjphysiol.7.42. [DOI] [PubMed] [Google Scholar]
  21. Katz B., Miledi R. A study of synaptic transmission in the absence of nerve impulses. J Physiol. 1967 Sep;192(2):407–436. doi: 10.1113/jphysiol.1967.sp008307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Katz B., Miledi R. Release of acetylcholine from a nerve terminal by electric pulses of variable strength and duration. Nature. 1965 Sep 4;207(5001):1097–1098. doi: 10.1038/2071097a0. [DOI] [PubMed] [Google Scholar]
  23. Katz B., Miledi R. Tetrodotoxin and neuromuscular transmission. Proc R Soc Lond B Biol Sci. 1967 Jan 31;167(1006):8–22. doi: 10.1098/rspb.1967.0010. [DOI] [PubMed] [Google Scholar]
  24. Katz B., Miledi R. The release of acetylcholine from nerve endings by graded electric pulses. Proc R Soc Lond B Biol Sci. 1967 Jan 31;167(1006):23–38. doi: 10.1098/rspb.1967.0011. [DOI] [PubMed] [Google Scholar]
  25. Koketsu K., Nishi S. Calcium spikes of nerve cell membrane: role of calcium in the production of action potentials. Nature. 1968 Feb 3;217(5127):468–469. doi: 10.1038/217468b0. [DOI] [PubMed] [Google Scholar]
  26. LI C. L. Cortical intracellular potentials and their responses to strychnine. J Neurophysiol. 1959 Jul;22(4):436–450. doi: 10.1152/jn.1959.22.4.436. [DOI] [PubMed] [Google Scholar]
  27. Miledi R. Spontaneous synaptic potentials and quantal release of transmitter in the stellate ganglion of the squid. J Physiol. 1967 Sep;192(2):379–406. doi: 10.1113/jphysiol.1967.sp008306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. NARAHASHI T., DEGUCHI T., URAKAWA N., OHKUBO Y. Stabilization and rectification of muscle fiber membrane by tetrodotoxin. Am J Physiol. 1960 May;198:934–938. doi: 10.1152/ajplegacy.1960.198.5.934. [DOI] [PubMed] [Google Scholar]
  29. NARAHASHI T., MOORE J. W., SCOTT W. R. TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS. J Gen Physiol. 1964 May;47:965–974. doi: 10.1085/jgp.47.5.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. NISHI S., KOKETSU K. Electrical properties and activities of single sympathetic neurons in frogs. J Cell Comp Physiol. 1960 Feb;55:15–30. doi: 10.1002/jcp.1030550104. [DOI] [PubMed] [Google Scholar]
  31. Nakamura Y., Nakajima S., Grundfest H. The action of tetrodotoxin on electrogenic components of squid giant axons. J Gen Physiol. 1965 Jul;48(6):975–996. [PubMed] [Google Scholar]

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