Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1967 May;190(1):19–34. doi: 10.1113/jphysiol.1967.sp008190

The in vitro transfer of bovine immune lactoglobulin across the intestine of new-born pigs

A E Pierce, M W Smith
PMCID: PMC1365401  PMID: 6038020

Abstract

1. Everted sacs of pig intestine, used soon after birth, maintained transmural potentials and transferred water and glucose to the serosal surface.

2. Immune globulin, fed as bovine colostrum to the new-born pig, appeared in the serosal fluid of everted sacs during incubation in bicarbonate saline. The particular segment showing maximum transferring ability varied between limits and appeared to depend on the amount or concentration of colostrum fed to the pig. Sacs from unfed pigs incubated in bovine colostrum also transferred colostral IgG to the serosal fluid. This transfer was dependent on the concentration of colostral IgG in the incubation medium and became more pronounced in the middle third of the small intestine.

3. Human serum albumin inhibited the transfer of colostral IgG and about twenty molecules of albumin were transferred for every molecule of colostral IgG, when both were presented together in equal concentration on the basis of weight, to the middle segment of the small intestine.

4. Some of the immune globulin collected in vitro after feeding bovine colostrum was found in a degraded form, but the amounts present could not be estimated. There was no apparent degradation of immune globulin in the purely in vitro experiments.

5. The in vitro transfer of bovine colostral IgG showed selectivity between molecules of albumin and colostral IgG, the nature of which warrants further study.

Full text

PDF
19

Images in this article

23Fig. 1
on p.23
24Fig. 2
on p.24
29Fig. 5
on p.29

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER R. D., SEARLE G. W., NUNN A. S. Glucose and sorbose absorption at various levels of rat small intestine. Am J Physiol. 1961 Feb;200:301–304. doi: 10.1152/ajplegacy.1961.200.2.301. [DOI] [PubMed] [Google Scholar]
  2. BARRY B. A., MATTHEWS J., SMYTH D. H. Transfer of glucose and fluid by different parts of the small intestine of the rat. J Physiol. 1961 Jul;157:279–288. doi: 10.1113/jphysiol.1961.sp006721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRAMBELL F. W., HALLIDAY R., MORRIS I. G. Interference by human and bovine serum and serum protein fractions with the absorption of antibodies by suckling rats and mice. Proc R Soc Lond B Biol Sci. 1958 Jul 1;149(934):1–11. doi: 10.1098/rspb.1958.0046. [DOI] [PubMed] [Google Scholar]
  4. Balfour W. E., Comline R. S. Acceleration of the absorption of unchanged globulin in the new-born calf by factors in colostrum. J Physiol. 1962 Feb;160(2):234–257. doi: 10.1113/jphysiol.1962.sp006844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLARK S. L., Jr The ingestion of proteins and colloidal materials by columnar absorptive cells of the small intestine in suckling rats and mice. J Biophys Biochem Cytol. 1959 Jan 25;5(1):41–50. doi: 10.1083/jcb.5.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GELL P. G. The estimation of the individual human serum proteins by an immunological method. J Clin Pathol. 1957 Feb;10(1):67–71. doi: 10.1136/jcp.10.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HANSEN O. Specificity of the glucose oxidase reaction and interference with the quantitative glucose oxidase-peroxidase-O-dianisidine method. Scand J Clin Lab Invest. 1962;14:651–655. doi: 10.1080/00365516209051298. [DOI] [PubMed] [Google Scholar]
  8. Harpur R. P., Popkin J. S. Osmolality of blood and intestinal contents in the pig, guinea pig, and Ascaris lumbricoides. Can J Biochem. 1965 Jul;43(7):1157–1169. doi: 10.1139/o65-128. [DOI] [PubMed] [Google Scholar]
  9. Mansford K. R. A preparation of surviving small intestine for the study of absorption in mammalian species. J Physiol. 1965 Oct;180(4):722–734. doi: 10.1113/jphysiol.1965.sp007726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  11. PAYNE L. C., MARSH C. L. Absorption of gamma globulin by the small intestine. Fed Proc. 1962 Nov-Dec;21:909–912. [PubMed] [Google Scholar]
  12. Pierce A. E., Smith M. W. The intestinal absorption of pig and bovine immune lactoglobulin and human serum albumin by the new-born pig. J Physiol. 1967 May;190(1):1–18. doi: 10.1113/jphysiol.1967.sp008189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STRAUSS E. W. The absorption of fat by intestine of golden hamster in vitro. J Cell Biol. 1963 Jun;17:597–607. doi: 10.1083/jcb.17.3.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WILSON T. H., LIN E. C. Active transport by intestines of fetal and newborn rabbits. Am J Physiol. 1960 Dec;199:1030–1032. doi: 10.1152/ajplegacy.1960.199.6.1030. [DOI] [PubMed] [Google Scholar]
  15. WILSON T. H., WISEMAN G. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J Physiol. 1954 Jan;123(1):116–125. doi: 10.1113/jphysiol.1954.sp005036. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES