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. 1967 Dec;193(3):481–496.3. doi: 10.1113/jphysiol.1967.sp008373

Cross-innervated mammalian skeletal muscle: histochemical, physiological and biochemical observations

Victor Dubowitz
PMCID: PMC1365508  PMID: 16992291

Abstract

1. Cross-innervation of the slow soleus and fast flexor hallucis longus or flexor digitorum longus muscles has been performed in new-born kittens and rabbits and in adult cats.

2. The effects on the histochemical and structural properties of the muscle have been studied and compared with the changes in the contractile properties.

3. Cross-innervation has produced a dramatic change in histochemical pattern in the fast muscles, with the development of areas of muscle fibres indistinguishable from normal soleus muscle. The converse change from the histochemical pattern of slow soleus to that of fast muscle has also occurred, but has been less consistent.

4. It is concluded that the neural influence determining the contractile properties of fast and slow muscle also has a profound controlling influence on the structure and metabolic activity of the muscle fibres.

5. No significant changes could be demonstrated biochemically in the ATPase activities of the fast and slow muscles following cross-innervation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN P., SEARS T. A. THE MECHANICAL PROPERTIES AND INNERVATION OF FAST AND SLOW MOTOR UNITS IN THE INTERCOSTAL MUSCLES OF THE CAT. J Physiol. 1964 Sep;173:114–129. doi: 10.1113/jphysiol.1964.sp007446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARANY M., BARANY K., RECKARD T., VOLPE A. MYOSIN OF FAST AND SLOW MUSCLES OF THE RABBIT. Arch Biochem Biophys. 1965 Jan;109:185–191. doi: 10.1016/0003-9861(65)90304-8. [DOI] [PubMed] [Google Scholar]
  3. BARANY M., GAETJENS E., BARANY K., KARP E. COMPARATIVE STUDIES OF RABBIT CARDIAC AND SKELETAL MYOSINS. Arch Biochem Biophys. 1964 Jul 20;106:280–293. doi: 10.1016/0003-9861(64)90189-4. [DOI] [PubMed] [Google Scholar]
  4. BESSOU P., EMONET-DENAND F., LAPORTE Y. [Relation between the conduction rate of motor nerve fibers and the contraction time of their motor units]. C R Hebd Seances Acad Sci. 1963 Jun 24;256:5625–5627. [PubMed] [Google Scholar]
  5. BULLER A. J., ECCLES J. C., ECCLES R. M. Differentiation of fast and slow muscles in the cat hind limb. J Physiol. 1960 Feb;150:399–416. doi: 10.1113/jphysiol.1960.sp006394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BULLER A. J., ECCLES J. C., ECCLES R. M. Interactions between motoneurones and muscles in respect of the characteristic speeds of their responses. J Physiol. 1960 Feb;150:417–439. doi: 10.1113/jphysiol.1960.sp006395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BULLER A. J., LEWIS D. M. FURTHER OBSERVATIONS ON MAMMALIAN CROSS-INNERVATED SKELETAL MUSCLE. J Physiol. 1965 May;178:343–358. doi: 10.1113/jphysiol.1965.sp007631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BULLER A. J., LEWIS D. M. SOME OBSERVATIONS ON THE EFFECTS OF TENOTOMY IN THE RABBIT. J Physiol. 1965 May;178:326–342. doi: 10.1113/jphysiol.1965.sp007630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. BULLER A. J., LEWIS D. M. THE RATE OF TENSION DEVELOPMENT IN ISOMETRIC TETANIC CONTRACTIONS OF MAMMALIAN FAST AND SLOW SKELETAL MUSCLE. J Physiol. 1965 Feb;176:337–354. doi: 10.1113/jphysiol.1965.sp007554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Bocek R. M., Beatty C. H. Glycogen synthetase and phosphorylase in red and white muscle of rat and rhesus monkey. J Histochem Cytochem. 1966 Jul;14(7):549–559. doi: 10.1177/14.7.549. [DOI] [PubMed] [Google Scholar]
  11. CLOSE R. DYNAMIC PROPERTIES OF FAST AND SLOW SKELETAL MUSCLES OF THE RAT DURING DEVELOPMENT. J Physiol. 1964 Sep;173:74–95. doi: 10.1113/jphysiol.1964.sp007444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Close R. Effects of cross-union of motor nerves to fast and slow skeletal muscles. Nature. 1965 May 22;206(4986):831–832. doi: 10.1038/206831a0. [DOI] [PubMed] [Google Scholar]
  13. Close R. Force: velocity properties of mouse muscles. Nature. 1965 May 15;206(985):718–719. doi: 10.1038/206718a0. [DOI] [PubMed] [Google Scholar]
  14. Cosmos E. Enzymatic activity of differentiating muscle fibers. I. Development of phosphorylase in muscles of the domestic fowl. Dev Biol. 1966 Apr;13(2):163–181. doi: 10.1016/0012-1606(66)90062-5. [DOI] [PubMed] [Google Scholar]
  15. DUBOWITZ V., PEARSE A. G. A comparative histochemical study of oxidative enzyme and phosphorylase activity in skeletal muscle. Z Zellforch Microsk Anat Histochem. 1960;2:105–117. doi: 10.1007/BF00744575. [DOI] [PubMed] [Google Scholar]
  16. DUBOWITZ V., PEARSE A. G. Reciprocal relationship of phosphorylase and oxidative enzymes in skeletal muscle. Nature. 1960 Mar 5;185:701–702. doi: 10.1038/185701a0. [DOI] [PubMed] [Google Scholar]
  17. Dubowitz V. Pathology of experimentally re-innervated skeletal muscle. J Neurol Neurosurg Psychiatry. 1967 Apr;30(2):99–110. doi: 10.1136/jnnp.30.2.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ENGEL W. K., CUNNINGHAM G. G. RAPID EXAMINATION OF MUSCLE TISSUE. AN IMPROVED TRICHROME METHOD FOR FRESH-FROZEN BIOPSY SECTIONS. Neurology. 1963 Nov;13:919–923. doi: 10.1212/wnl.13.11.919. [DOI] [PubMed] [Google Scholar]
  19. ERANKO O., PALKAMA A. Improved localization of phosphorylase by the use of polyvinyl pyrrolidone and high substrate concentration. J Histochem Cytochem. 1961 Sep;9:585–585. doi: 10.1177/9.5.585. [DOI] [PubMed] [Google Scholar]
  20. GORDON G., PHILLIPS C. G. Slow and rapid components in a flexor muscle. Q J Exp Physiol Cogn Med Sci. 1953;38(1):35–45. doi: 10.1113/expphysiol.1953.sp001005. [DOI] [PubMed] [Google Scholar]
  21. Maclagan J., Vrbová G. The importance of peripheral changes in determining the sensitivity of striated muscle to depolarizing drugs. J Physiol. 1966 Jun;184(3):618–630. doi: 10.1113/jphysiol.1966.sp007935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Miledi R., Orkand P. Effect of a "fast" nerve on "slow" muscle fibres in the frog. Nature. 1966 Feb 12;209(5024):717–718. doi: 10.1038/209717a0. [DOI] [PubMed] [Google Scholar]
  23. PADYKULA H. A., HERMAN E. The specificity of the histochemical method for adenosine triphosphatase. J Histochem Cytochem. 1955 May;3(3):170–195. doi: 10.1177/3.3.170. [DOI] [PubMed] [Google Scholar]
  24. PATON W. D. M., ZAIMIS E. J. The action of d-tubocurarine and of decamethonium on respiratory and other muscles in the cat. J Physiol. 1951 Feb;112(3-4):311–331. doi: 10.1113/jphysiol.1951.sp004531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SCARPELLI D. G., HESS R., PEARSE A. G. The cytochemical localization of oxidative enzymes. I. Diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase. J Biophys Biochem Cytol. 1958 Nov 25;4(6):747–752. doi: 10.1083/jcb.4.6.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. STEIN J. M., PADYKULA H. A. Histochemical classification of individual skeletal muscle fibers of the rat. Am J Anat. 1962 Mar;110:103–123. doi: 10.1002/aja.1001100203. [DOI] [PubMed] [Google Scholar]
  27. Sawyer D., Sie H. G., Fishman W. H. A technic for preparing permanent histochemical preparations of liver phosphorylase. J Histochem Cytochem. 1965 Sep-Oct;13(7):605–607. doi: 10.1177/13.7.605. [DOI] [PubMed] [Google Scholar]
  28. TAKEUCHI T., KURIAKI H. Histochemical detection of phosphorylase in animal tissues. J Histochem Cytochem. 1955 May;3(3):153–160. doi: 10.1177/3.3.153. [DOI] [PubMed] [Google Scholar]
  29. VRBOVA G. Changes in the motor reflexes produced by tenotomy. J Physiol. 1963 Apr;166:241–250. doi: 10.1113/jphysiol.1963.sp007103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. VRBOVA G. THE EFFECT OF MOTONEURONE ACTIVITY ON THE SPEED OF CONTRACTION OF STRIATED MUSCLE. J Physiol. 1963 Dec;169:513–526. doi: 10.1113/jphysiol.1963.sp007276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WEBER A. The ultracentrifugal separation of L-myosin and actin in an actomyosin sol under the influence of ATP. Biochim Biophys Acta. 1956 Feb;19(2):345–351. doi: 10.1016/0006-3002(56)90439-5. [DOI] [PubMed] [Google Scholar]

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